Detailed view for Rv0808

Basic information

TDR Targets ID: 981190
Mycobacterium tuberculosis, Amidophosphoribosyltransferase PurF (glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) (gpatase)
Source Database / ID:  Tuberculist 

pI: 5.0496 | Length (AA): 527 | MW (Da): 56130 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00156  
PF13522  

Gene Ontology

Mouse over links to read term descriptions.
GO:0004044   amidophosphoribosyltransferase activity  
GO:0009116   nucleoside metabolic process  
GO:0009113   purine base biosynthetic process  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
35 512 1ecf (A) 1 489 36.00 0 1 1.30712 -0.22
35 496 1ao0 (A) 1 455 47.00 0 1 1.44926 -0.5
292 411 1g2q (A) 37 155 18.00 0 0.66 0.502604 -0.71

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127706)

Species Accession Gene Product
Arabidopsis thaliana AT4G34740   glutamine 5-phosphoribosylpyrophosphate amidotransferase
Arabidopsis thaliana AT4G38880   amidophosphoribosyltransferase 3
Arabidopsis thaliana AT2G16570   amidophosphoribosyltransferase 1
Candida albicans CaO19.1233   Amidophosphoribosyltransferase
Candida albicans CaO19.8818   Amidophosphoribosyltransferase
Caenorhabditis elegans CELE_T04A8.5   Protein T04A8.5
Dictyostelium discoideum DDB_G0274321   phosphoribosylpyrophosphate amidotransferase
Drosophila melanogaster Dmel_CG10078   Phosphoribosylamidotransferase 2
Drosophila melanogaster Dmel_CG2867   Phosphoribosylamidotransferase
Escherichia coli b2312   amidophosphoribosyltransferase
Homo sapiens ENSG00000128059   phosphoribosyl pyrophosphate amidotransferase
Mycobacterium leprae ML2206c   AMIDOPHOSPHORIBOSYLTRANSFERASE PURF (GLUTAMINE PHOSPHORIBOSYLPYROPHOSPHATE AMIDOTRANSFERASE) (ATASE) (GPATASE)
Mus musculus ENSMUSG00000029246   phosphoribosyl pyrophosphate amidotransferase
Mycobacterium tuberculosis Rv0808   Amidophosphoribosyltransferase PurF (glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) (gpatase)
Mycobacterium ulcerans MUL_0417   amidophosphoribosyltransferase
Oryza sativa 4338879   Os05g0430800
Saccharomyces cerevisiae YMR300C   amidophosphoribosyltransferase
Schmidtea mediterranea mk4.009832.00   Amidophosphoribosyltransferase
Schmidtea mediterranea mk4.011031.00   Amidophosphoribosyltransferase
Schmidtea mediterranea mk4.010079.00   Amidophosphoribosyltransferase
Schmidtea mediterranea mk4.004315.03   Amidophosphoribosyltransferase
Schmidtea mediterranea mk4.004315.02   Amidophosphoribosyltransferase
Schmidtea mediterranea mk4.003339.05   Amidophosphoribosyltransferase
Wolbachia endosymbiont of Brugia malayi Wbm0255   amidophosphoribosyltransferase

Essentiality

Rv0808 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b2312 Escherichia coli non-essential goodall
CELE_T04A8.5 Caenorhabditis elegans larval arrest wormbase
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0808 (Mycobacterium tuberculosis), Amidophosphoribosyltransferase PurF (glutamine phosphoribosylpyrophosphate amidotransferase) (ATASE) (gpatase)
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