Detailed view for Rv0843

Basic information

TDR Targets ID: 980343
Mycobacterium tuberculosis, Probable dehydrogenase
Source Database / ID:  Tuberculist 

pI: 5.8665 | Length (AA): 334 | MW (Da): 35725 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00676  

Gene Ontology

Mouse over links to read term descriptions.
GO:0016624   oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 329 2bfd (A) 45 374 22.00 0.00000000042 1 1.19755 0.2
14 313 1umd (A) 36 339 28.00 0 1 1.2833 -0.55
14 331 1umd (A) 36 353 25.00 0.0000017 1 1.3126 -0.29
115 265 3m49 (A) 109 265 34.00 0.012 1 0.696896 0.58

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127216)

Species Accession Gene Product
Arabidopsis thaliana AT1G24180   pyruvate dehydrogenase E1 component subunit alpha-2
Arabidopsis thaliana AT1G59900   pyruvate dehydrogenase complex E1 alpha subunit
Arabidopsis thaliana AT1G01090   pyruvate dehydrogenase E1 alpha subunit
Brugia malayi Bm1_25865   pyruvate dehydrogenase E1 component alpha subunit, mitochondrial
Brugia malayi Bm1_25860   probable pyruvate dehydrogenase E1 component alpha subunit, mitochondrial
Candida albicans CaO19.10609   alpha subunit of pyruvate dehydrogenase
Candida albicans CaO19.3097   alpha subunit of pyruvate dehydrogenase
Caenorhabditis elegans CELE_T05H10.6   Protein PDHA-1, isoform A
Chlamydia trachomatis CT_245   pyruvate dehydrogenase subunit alpha
Dictyostelium discoideum DDB_G0292994   pyruvate dehydrogenase E1 alpha subunit
Drosophila melanogaster Dmel_CG7024   CG7024 gene product from transcript CG7024-RA
Drosophila melanogaster Dmel_CG7010   lethal (1) G0334
Echinococcus granulosus EgrG_000590700   pyruvate dehydrogenase E1 component subunit
Echinococcus multilocularis EmuJ_000590700   pyruvate dehydrogenase E1 component subunit
Homo sapiens ENSG00000163114   pyruvate dehydrogenase (lipoamide) alpha 2
Homo sapiens ENSG00000131828   pyruvate dehydrogenase (lipoamide) alpha 1
Leishmania braziliensis LbrM.18.1410   pyruvate dehydrogenase E1 component alpha subunit, putative
Leishmania donovani LdBPK_181360.1   pyruvate dehydrogenase E1 component alpha subunit, putative
Leishmania infantum LinJ.18.1360   pyruvate dehydrogenase E1 component alpha subunit, putative
Leishmania major LmjF.18.1380   pyruvate dehydrogenase E1 component alpha subunit, putative
Leishmania mexicana LmxM.18.1380   pyruvate dehydrogenase E1 component alpha subunit, putative
Loa Loa (eye worm) LOAG_04006   pyruvate dehydrogenase E1 component alpha subunit
Loa Loa (eye worm) LOAG_04007   pyruvate dehydrogenase E1 component alpha subunit
Mus musculus ENSMUSG00000047674   pyruvate dehydrogenase E1 alpha 2
Mus musculus ENSMUSG00000031299   pyruvate dehydrogenase E1 alpha 1
Mycobacterium tuberculosis Rv0843   Probable dehydrogenase
Neospora caninum NCLIV_062940   pyruvate dehydrogenase, putative
Oryza sativa 4330673   Os02g0739600
Oryza sativa 4334968   Os04g0119400
Oryza sativa 4340640   Os06g0246500
Plasmodium berghei PBANKA_0923800   pyruvate dehydrogenase E1 component subunit alpha
Plasmodium falciparum PF3D7_1124500   pyruvate dehydrogenase E1 component subunit alpha
Plasmodium knowlesi PKNH_0922300   pyruvate dehydrogenase E1 component subunit alpha, putative
Plasmodium vivax PVX_091840   pyruvate dehydrogenase E1 component subunit alpha, putative
Plasmodium yoelii PY00819   pyruvate dehydrogenase E1 alpha subunit
Saccharomyces cerevisiae YER178W   pyruvate dehydrogenase (acetyl-transferring) subunit E1 alpha
Schistosoma japonicum Sjp_0055140   ko:K00161 pyruvate dehydrogenase E1 component, alpha subunit [EC1.2.4.1A], putative
Schistosoma mansoni Smp_079920   pyruvate dehydrogenase
Schmidtea mediterranea mk4.000104.17   Probable pyruvate dehydrogenase E1 component subunit alpha, mitochondrial
Trypanosoma brucei gambiense Tbg972.10.15320   pyruvate dehydrogenase E1 component alpha subunit, putative
Trypanosoma brucei Tb927.10.12700   pyruvate dehydrogenase E1 alpha subunit, putative
Trypanosoma congolense TcIL3000_10_10810   pyruvate dehydrogenase E1 component alpha subunit, putative
Trypanosoma cruzi TcCLB.507831.70   pyruvate dehydrogenase E1 component alpha subunit, putative
Toxoplasma gondii TGME49_245670   pyruvate dehydrogenase complex subunit PDH-E1Alpha
Wolbachia endosymbiont of Brugia malayi Wbm0207   pyruvate/2-oxoglutarate dehydrogenase complex, dehydrogenase E1 component, alpha subunit

Essentiality

Rv0843 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.12700 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.12700 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.12700 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.12700 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_T05H10.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_T05H10.6 Caenorhabditis elegans slow growth wormbase
CELE_T05H10.6 Caenorhabditis elegans sterile wormbase
PBANKA_0923800 Plasmodium berghei Essential plasmo
TGME49_245670 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0843 (Mycobacterium tuberculosis), Probable dehydrogenase
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