Detailed view for Rv0861c

Basic information

TDR Targets ID: 981680
Mycobacterium tuberculosis, DNA helicase Ercc3
Source Database / ID:  Tuberculist 

pI: 5.9472 | Length (AA): 542 | MW (Da): 59772 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00271  
PF04851  
PF13625  

Gene Ontology

Mouse over links to read term descriptions.
GO:0016787   hydrolase activity  
GO:0005524   ATP binding  
GO:0004003   ATP-dependent DNA helicase activity  
GO:0003677   DNA binding  
GO:0006289   nucleotide-excision repair  

Metabolic Pathways

Nucleotide excision repair (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
108 514 5m52 (B) 373 897 27.00 0.95 1 0.799523 0.84
351 540 4ern (A) 502 696 34.00 0 1 0.824054 -1.02
398 470 5lm7 (A) 80 161 42.00 0.81 0.06 0.246286 1.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127208)

Species Accession Gene Product
Arabidopsis thaliana AT5G41360   DNA repair helicase XPB2
Arabidopsis thaliana AT5G41370   DNA repair helicase XPB1
Babesia bovis BBOV_III010030   DNA repair helicase rad25 family protein
Brugia malayi Bm1_20800   helicase
Candida albicans CaO19.2857   DNA helicase
Candida albicans CaO19.10376   DNA helicase
Caenorhabditis elegans CELE_Y66D12A.15   Protein Y66D12A.15
Cryptosporidium hominis Chro.80227   hypothetical protein
Cryptosporidium parvum cgd8_1940   RAD25, helicase involved in DNA repair
Dictyostelium discoideum DDB_G0278729   transcription factor IIH subunit
Drosophila melanogaster Dmel_CG8019   haywire
Echinococcus granulosus EgrG_000600500   rad25:xp B DNA repair helicase
Entamoeba histolytica EHI_088430   DNA repair helicase, putative
Entamoeba histolytica EHI_077260   DNA repair helicase, putative
Echinococcus multilocularis EmuJ_000600500   rad25:xp B DNA repair helicase
Giardia lamblia GL50803_16512   DNA repair helicase TFIIH P90
Homo sapiens ENSG00000163161   excision repair cross-complementation group 3
Leishmania braziliensis LbrM.29.0600   DNA repair helicase, putative
Leishmania braziliensis LbrM.32.4160   helicase, putative,DNA repair helicase, putative
Leishmania donovani LdBPK_290610.1   tfiih basal transcription factor complex helicase xpb subunit
Leishmania donovani LdBPK_324070.1   TFIIH basal transcription factor complex helicase XPB subunit, putative
Leishmania infantum LinJ.29.0610   DNA repair helicase, putative
Leishmania infantum LinJ.32.4070   helicase, putative,DNA repair helicase, putative
Leishmania major LmjF.32.3920   helicase, putative,DNA repair helicase, putative
Leishmania major LmjF.29.0590   DNA repair helicase, putative
Leishmania mexicana LmxM.08_29.0590   DNA repair helicase, putative
Leishmania mexicana LmxM.31.3920   helicase, putative,DNA repair helicase, putative
Loa Loa (eye worm) LOAG_00638   helicase
Mycobacterium leprae ML2157   PROBABLE DNA HELICASE ERCC3
Mus musculus ENSMUSG00000024382   excision repair cross-complementing rodent repair deficiency, complementation group 3
Mycobacterium tuberculosis Rv0861c   DNA helicase Ercc3
Mycobacterium ulcerans MUL_0289   DNA helicase Ercc3
Neospora caninum NCLIV_036860   hypothetical protein
Oryza sativa 4325640   Os01g0691600
Plasmodium berghei PBANKA_0520500   DNA repair helicase RAD25, putative
Plasmodium falciparum PF3D7_1037600   DNA repair helicase RAD25, putative
Plasmodium knowlesi PKNH_0622100   DNA repair helicase RAD25, putative
Plasmodium vivax PVX_110880   DNA repair helicase RAD25, putative
Plasmodium yoelii PY04074   RepB-related
Saccharomyces cerevisiae YIL143C   TFIIH/NER complex ATPase/helicase subunit SSL2
Schistosoma japonicum Sjp_0116080   ko:K01509 adenosinetriphosphatase [EC3.6.1.3], putative
Schistosoma japonicum Sjp_0104520   TFIIH basal transcription factor complex helicase XPB subunit, putative
Schistosoma mansoni Smp_165580   rad25/xp-B DNA repair helicase
Schmidtea mediterranea mk4.003577.00  
Schmidtea mediterranea mk4.004232.06  
Schmidtea mediterranea mk4.037825.00   TFIIH basal transcription factor complex helicase XPB subunit
Schmidtea mediterranea mk4.039578.00   TFIIH basal transcription factor complex helicase XPB subunit
Trypanosoma brucei gambiense Tbg972.3.5740   DNA repair helicase and transcription factor protein, putative,Transcription factor II H complex, XPB subunit, putative homologu
Trypanosoma brucei gambiense Tbg972.11.18270   DNA repair helicase and transcription factor protein, putative,DNA repair helicase, putative,transcription factor II H complex,
Trypanosoma brucei Tb927.3.5100   tfiih basal transcription factor complex helicase xpb subunit
Trypanosoma brucei Tb927.11.16270   TFIIH basal transcription factor complex helicase XPB subunit, putative
Trypanosoma congolense TcIL3000.11.16180   TFIIH basal transcription factor complex helicase XPB subunit, putative
Trypanosoma congolense TcIL3000_3_3090   tfiih basal transcription factor complex helicase xpb subunit
Trypanosoma cruzi TcCLB.508411.90   tfiih basal transcription factor complex helicase xpb subunit
Trypanosoma cruzi TcCLB.511527.20   DNA repair helicase and transcription factor protein, putative
Trypanosoma cruzi TcCLB.510149.50   tfiih basal transcription factor complex helicase xpb subunit
Toxoplasma gondii TGME49_269660   TFIIH basal transcription factor complex helicase XPB subunit
Toxoplasma gondii TGME49_302450   TFIIH basal transcription factor complex helicase XPB subunit, putative
Treponema pallidum TP0380   DNA repair helicase
Theileria parva TP02_0144   DNA helicase, putative
Trichomonas vaginalis TVAG_167010   rad25/xp-B DNA repair helicase, putative
Trichomonas vaginalis TVAG_044250   rad25/xp-B DNA repair helicase, putative

Essentiality

Rv0861c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.5100 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.3.5100 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.5100 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.5100 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.01.7950 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.7950 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.7950 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.01.7950 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y66D12A.15 Caenorhabditis elegans embryonic lethal wormbase
YIL143C Saccharomyces cerevisiae inviable yeastgenome
PBANKA_0520500 Plasmodium berghei Essential plasmo
TGME49_302450 Toxoplasma gondii Probably essential sidik
TGME49_269660 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv0861c (Mycobacterium tuberculosis), DNA helicase Ercc3
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