Detailed view for Rv1699

Basic information

TDR Targets ID: 981249
Mycobacterium tuberculosis, Probable CTP synthase PyrG
Source Database / ID:  Tuberculist 

pI: 5.4505 | Length (AA): 586 | MW (Da): 63603 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00117  
PF06418  

Gene Ontology

Mouse over links to read term descriptions.
GO:0003883   CTP synthase activity  
GO:0006221   pyrimidine nucleotide biosynthetic process  

Metabolic Pathways

Pyrimidine metabolism (KEGG)

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 552 4zdj (A) 5 552 99.99 0 1 2.15695 -1.52

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126851)

Species Accession Gene Product
Arabidopsis thaliana AT2G34890   CTP synthase family protein
Arabidopsis thaliana AT3G12670   CTP synthase family protein
Arabidopsis thaliana AT1G30820   CTP synthase-like protein
Arabidopsis thaliana AT4G02120   putative CTP synthase
Arabidopsis thaliana AT4G20320   putative CTP synthase
Babesia bovis BBOV_II005340   cytidine triphosphate synthetase, putative
Brugia malayi Bm1_27685   UTP--ammonia ligase
Candida albicans CaO19.3941   CTP synthase
Candida albicans CaO19.11423   CTP synthase
Caenorhabditis elegans CELE_W06H3.3   Protein CTPS-1
Cryptosporidium hominis Chro.50211   last step in pyrimidine biosynthesis pathway; Ura8p
Cryptosporidium parvum cgd5_1710   CTP synthetase (UTP-ammonia lyase)
Chlamydia trachomatis CT_183   CTP synthetase
Dictyostelium discoideum DDB_G0280567   CTP synthase
Drosophila melanogaster Dmel_CG45070   CTP synthase
Escherichia coli b2780   CTP synthetase
Echinococcus granulosus EgrG_000849200   CTP synthase 1
Echinococcus multilocularis EmuJ_000849200   CTP synthase 1
Giardia lamblia GL50803_17587   CTP synthase
Homo sapiens ENSG00000171793   CTP synthase 1
Homo sapiens ENSG00000047230   CTP synthase 2
Leishmania braziliensis LbrM.20.4800   cytidine triphosphate synthase, putative,CTP synthase, putative
Leishmania donovani LdBPK_200630.1   cytidine triphosphate synthase, putative
Leishmania infantum LinJ.20.0630   cytidine triphosphate synthase, putative,CTP synthase, putative
Leishmania major LmjF.20.0560   cytidine triphosphate synthase, putative
Leishmania mexicana LmxM.20.0560   cytidine triphosphate synthase, putative
Loa Loa (eye worm) LOAG_09164   hypothetical protein
Loa Loa (eye worm) LOAG_09165   hypothetical protein
Loa Loa (eye worm) LOAG_16327   hypothetical protein
Mycobacterium leprae ML1363   Probable CTP synthase PyrG
Mus musculus ENSMUSG00000031360   cytidine 5'-triphosphate synthase 2
Mus musculus ENSMUSG00000028633   cytidine 5'-triphosphate synthase
Mycobacterium tuberculosis Rv1699   Probable CTP synthase PyrG
Mycobacterium ulcerans MUL_1688   CTP synthetase
Neospora caninum NCLIV_009300   GK17769, related
Oryza sativa 4339656   Os05g0569700
Oryza sativa 4339675   Os05g0573300
Oryza sativa 4324172   Os01g0654100
Oryza sativa 4352499   Os12g0556600
Oryza sativa 4326656   Os01g0616900
Onchocerca volvulus OVOC13450  
Plasmodium berghei PBANKA_1032300   cytidine triphosphate synthetase, putative
Plasmodium falciparum PF3D7_1410200   cytidine triphosphate synthetase
Plasmodium knowlesi PKNH_1348100   cytidine triphosphate synthetase, putative
Plasmodium vivax PVX_085940   CTP synthase, putative
Plasmodium yoelii PY00624   CTP synthase
Saccharomyces cerevisiae YBL039C   CTP synthase URA7
Saccharomyces cerevisiae YJR103W   CTP synthase URA8
Schistosoma japonicum Sjp_0029010   CTP synthase, putative
Schistosoma japonicum Sjp_0103900   ko:K01937 CTP synthase [EC6.3.4.2], putative
Schistosoma mansoni Smp_134650   CTP synthase
Schmidtea mediterranea mk4.043208.01   CTP synthase
Schmidtea mediterranea mk4.004931.00   CTP synthase
Trypanosoma brucei gambiense Tbg972.1.580   cytidine triphosphate synthase, putative
Trypanosoma brucei Tb11.v5.0356   cytidine triphosphate synthase, putative
Trypanosoma congolense TcIL3000_0_17700   cytidine triphosphate synthase, putative
Trypanosoma cruzi TcCLB.508993.10   cytidine triphosphate synthase, putative
Toxoplasma gondii TGME49_299210   CTP synthase
Treponema pallidum TP0305   CTP synthetase
Theileria parva TP02_0838   cytidine triphosphate synthetase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0169   CTP synthetase

Essentiality

Rv1699 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.1240 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.1.1240 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.1.1240 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.1240 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2780 Escherichia coli essential goodall
CELE_W06H3.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_W06H3.3 Caenorhabditis elegans larval arrest wormbase
CELE_W06H3.3 Caenorhabditis elegans slow growth wormbase
CELE_W06H3.3 Caenorhabditis elegans sterile wormbase
PBANKA_1032300 Plasmodium berghei Essential plasmo
TGME49_299210 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Rv1699 (Mycobacterium tuberculosis), Probable CTP synthase PyrG
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