Detailed view for Rv1814

Basic information

TDR Targets ID: 5942
Mycobacterium tuberculosis, Membrane-bound C-5 sterol desaturase Erg3 (sterol-C5-desaturase)
Source Database / ID:  Tuberculist 

pI: 9.9428 | Length (AA): 300 | MW (Da): 34882 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 3

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF04116   Fatty acid hydroxylase superfamily

Gene Ontology

Mouse over links to read term descriptions.
GO:0005783   endoplasmic reticulum  
GO:0005506   iron ion binding  
GO:0016491   oxidoreductase activity  
GO:0006633   fatty acid biosynthetic process  
GO:0055114   oxidation reduction  
GO:0008610   lipid biosynthetic process  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. hasan
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_132934)

Species Accession Gene Product
Brugia malayi Bm1_24485   hypothetical protein
Caenorhabditis elegans CELE_BE10.2   Protein AGMO-1
Dictyostelium discoideum DDB_G0277137   SUR2-type hydroxylase/desaturase catalytic region-containing protein
Dictyostelium discoideum DDB_G0281443   hypothetical protein
Homo sapiens ENSG00000187546   alkylglycerol monooxygenase
Loa Loa (eye worm) LOAG_12991   hypothetical protein
Mus musculus ENSMUSG00000050103   alkylglycerol monooxygenase
Mycobacterium tuberculosis Rv1814   Membrane-bound C-5 sterol desaturase Erg3 (sterol-C5-desaturase)
Mycobacterium ulcerans MUL_3066   membrane-bound C-5 sterol desaturase Erg3
Schmidtea mediterranea mk4.020158.02   Alkylglycerol monooxygenase homolog

Essentiality

Rv1814 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu1844 this record Mycobacterium tuberculosis non-essential nmpdr
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv1814 (Mycobacterium tuberculosis), Membrane-bound C-5 sterol desaturase Erg3 (sterol-C5-desaturase)
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