Detailed view for Rv2204c

Basic information

TDR Targets ID: 8405
Mycobacterium tuberculosis, Conserved protein
Source Database / ID:  Tuberculist 

pI: 4.1856 | Length (AA): 118 | MW (Da): 12544 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01521   Iron-sulphur cluster biosynthesis

Gene Ontology

Mouse over links to read term descriptions.
GO:0097428   GO:protein maturation by iron-sulfur cluster transfer  

GO:0051536   iron-sulfur cluster binding  
GO:0005198   structural molecule activity  
GO:0016226   iron-sulfur cluster assembly  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
5 118 2d2a (A) 9 121 32.00 0 1 1.3953 -0.46
12 109 1r94 (A) 1 97 33.00 0.00000000002 1 1.29951 -0.59
13 118 2apn (A) 8 113 44.00 0 1 1.44331 -0.15

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_127463)

Species Accession Gene Product
Arabidopsis thaliana AT5G03905   Iron-sulfur assembly protein IscA-like 2
Babesia bovis BBOV_III007130   HesB-like domain containing protein
Babesia bovis BBOV_III006900   HesB-like domain containing protein
Brugia malayi Bm1_18330   Iron-sulfur cluster assembly accessory protein
Brugia malayi Bm1_04165   Iron-sulfur cluster assembly accessory protein
Candida albicans CaO19.14103   similar to Iron Sulfur cluster Assembly protein
Candida albicans CaO19.6811   similar to Iron Sulfur cluster Assembly protein
Caenorhabditis elegans CELE_Y54G11A.9   Protein Y54G11A.9
Dictyostelium discoideum DDB_G0284809   HesB/YadR/YfhF domain-containing protein
Drosophila melanogaster Dmel_CG13623   CG13623 gene product from transcript CG13623-RA
Escherichia coli b0156   iron-sulfur cluster insertion protein
Echinococcus granulosus EgrG_000211050   iron sulfur cluster assembly 2
Echinococcus multilocularis EmuJ_000211050   iron sulfur cluster assembly 2
Giardia lamblia GL50803_14821   HesB domain-containing protein
Homo sapiens 122961   iron-sulfur cluster assembly 2
Leishmania braziliensis LbrM.34.1260   hypothetical protein, conserved
Leishmania donovani LdBPK_351350.1   Iron-sulfur assembly protein 2, putative
Leishmania infantum LinJ.35.1350   hypothetical protein, conserved
Leishmania major LmjF.35.1350   hypothetical protein, conserved
Leishmania mexicana LmxM.34.1350   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_08328   iron-sulfur cluster assembly accessory protein
Mycobacterium leprae ML0871   conserved hypothetical protein
Mus musculus ENSMUSG00000021241   iron-sulfur cluster assembly 2 homolog (S. cerevisiae)
Mycobacterium tuberculosis Rv2204c   Conserved protein
Mycobacterium ulcerans MUL_3561   hypothetical protein
Oryza sativa 4340113   Os06g0146400
Oryza sativa 9271865   Os08g0363800
Oryza sativa 4345405   Os08g0369700
Plasmodium berghei PBANKA_0304600   iron-sulfur assembly protein, putative
Plasmodium berghei PBANKA_1237400   iron-sulfur assembly protein
Plasmodium falciparum PF3D7_0207200   iron-sulfur assembly protein, putative
Plasmodium falciparum PF3D7_0522700   iron-sulfur assembly protein, putative
Plasmodium knowlesi PKNH_0413900   iron-sulfur assembly protein, putative
Plasmodium vivax PVX_003785   iron-sulfur cluster assembly accessory protein, putative
Plasmodium yoelii PY02064   member hesB family
Plasmodium yoelii PY00043   HesB-like domain, putative
Saccharomyces cerevisiae YPR067W   Isa2p
Schistosoma japonicum Sjp_0059250   Iron-sulfur cluster assembly 2 homolog, mitochondrial precursor, putative
Schistosoma mansoni Smp_034530.2   hes-B related
Schmidtea mediterranea mk4.001801.03   Iron-sulfur cluster assembly 2 homolog, mitochondrial
Trypanosoma brucei gambiense Tbg972.5.1400   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.1030   Iron-sulfur assembly protein 2
Trypanosoma congolense TcIL3000_5_780   Iron-sulfur assembly protein 2, putative
Trypanosoma cruzi TcCLB.511181.30   Iron-sulfur assembly protein 2, putative
Trypanosoma cruzi TcCLB.503465.30   Iron-sulfur assembly protein 2, putative
Theileria parva TP04_0596   hypothetical protein
Theileria parva TP04_0597   hypothetical protein
Trichomonas vaginalis TVAG_456770   iron-sulfur cluster assembly protein, putative
Trichomonas vaginalis TVAG_055320   iron sulfur assembly protein, putative
Trichomonas vaginalis TVAG_361540   iron-sulfur assembly protein, putative
Trichomonas vaginalis TVAG_373690   conserved hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0024   HesB/YadR/YfhF family protein

Essentiality

Rv2204c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2238 this record Mycobacterium tuberculosis non-essential nmpdr
Tb927.5.1030 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.1030 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.1030 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.1030 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0156 Escherichia coli essential goodall
PBANKA_0304600 Plasmodium berghei Essential plasmo
PBANKA_1237400 Plasmodium berghei Dispensable plasmo
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Rv2204c (Mycobacterium tuberculosis), Conserved protein
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