Detailed view for Rv2284

Basic information

TDR Targets ID: 980315
Mycobacterium tuberculosis, Probable esterase LipM
Source Database / ID:  Tuberculist 

pI: 9.5689 | Length (AA): 431 | MW (Da): 46681 | Paralog Number: 3

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 3

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF07859  

Gene Ontology

Mouse over links to read term descriptions.
GO:0016787   hydrolase activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
66 414 3fnb (A) 43 397 13.00 0 1 0.822345 0.24
100 413 5jd5 (A) 15 318 22.00 0 0.97 1.00354 -0.04
159 263 4be9 (A) 104 222 34.00 0.072 0.99 0.61262 -0.76
181 293 1c7j (A) 98 227 41.00 0.0000019 1 0.613881 -0.53

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130169)

Species Accession Gene Product
Arabidopsis thaliana AT1G26120   probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL1
Arabidopsis thaliana AT3G02410   probable isoprenylcysteine alpha-carbonyl methylesterase ICMEL2
Arabidopsis thaliana AT5G15860   prenylcysteine methylesterase
Dictyostelium discoideum DDB_G0292226   hypothetical protein
Leishmania braziliensis LbrM.15.0360   ecotin, putative
Leishmania donovani LdBPK_150370.1   Isoprenylcysteine alpha-carbonyl methylesterase, putative
Leishmania infantum LinJ.15.0370   ecotin, putative
Leishmania major LmjF.15.0320   ecotin, putative
Leishmania mexicana LmxM.15.0320   hypothetical protein, conserved
Mycobacterium tuberculosis Rv2284   Probable esterase LipM
Mycobacterium tuberculosis Rv0220   Probable esterase LipC
Mycobacterium tuberculosis Rv1426c   Probable esterase LipO
Mycobacterium tuberculosis Rv2485c   Probable carboxylesterase LipQ
Mycobacterium ulcerans MUL_2704   membrane-bound esterase LipM
Mycobacterium ulcerans MUL_1112   esterase LipC
Mycobacterium ulcerans MUL_3766   carboxylesterase LipQ
Mycobacterium ulcerans MUL_1819   esterase LipO
Oryza sativa 4339700   Os05g0577200
Oryza sativa 4326560   Os01g0642000
Oryza sativa 4342018   Os06g0708100
Trypanosoma brucei gambiense Tbg972.5.2460   hypothetical protein, conserved
Trypanosoma brucei Tb927.5.1760   Isoprenylcysteine alpha-carbonyl methylesterase, putative
Trypanosoma congolense TcIL3000_0_36010   Isoprenylcysteine alpha-carbonyl methylesterase, putative
Trypanosoma congolense TcIL3000_0_45730   Isoprenylcysteine alpha-carbonyl methylesterase, putative
Trypanosoma cruzi TcCLB.505229.10   Isoprenylcysteine alpha-carbonyl methylesterase, putative
Trypanosoma cruzi TcCLB.510823.30   Isoprenylcysteine alpha-carbonyl methylesterase, putative

Essentiality

Rv2284 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu222 Mycobacterium tuberculosis non-essential nmpdr
mtu1449 Mycobacterium tuberculosis non-essential nmpdr
mtu2528 Mycobacterium tuberculosis non-essential nmpdr
Tb927.5.1760 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.1760 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.1760 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.5.1760 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2284 (Mycobacterium tuberculosis), Probable esterase LipM
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