Detailed view for Rv2338c

Basic information

TDR Targets ID: 6516
Mycobacterium tuberculosis, Possible molybdopterin biosynthesis protein MoeW
Source Database / ID:  Tuberculist 

pI: 9.6789 | Length (AA): 318 | MW (Da): 35271 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00899   ThiF family

Gene Ontology

Mouse over links to read term descriptions.
GO:0008641   small protein activating enzyme activity  
GO:0005488   binding  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
17 287 1jw9 (B) 2 247 22.00 0.0036 1 0.959201 0.25
17 231 1jwa (B) 2 240 21.00 0.63 1 0.969101 -0.21
20 253 4d79 (A) 8 262 24.00 0 1 1.01675 -0.15
38 108 5iaa (A) 69 142 37.00 0.00014 0.63 0.70467 -0.57

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_129035)

Species Accession Gene Product
Arabidopsis thaliana AT5G37530   NAD(P)-binding Rossmann-fold superfamily protein
Candida albicans CaO19.9663   similar to E. coli molybdopterin-converting factor
Candida albicans CaO19.2115   similar to E. coli molybdopterin-converting factor
Dictyostelium discoideum DDB_G0268496   hypothetical protein
Escherichia coli b2812   tRNA threonylcarbamoyladenosine dehydratase
Entamoeba histolytica EHI_188810   HesA/MoeB/ThiF family protein, putative
Giardia lamblia GL50803_11436   Molybdopterin biosynthesis MoeB protein
Leishmania donovani LdBPK_240470.1   ubiquitin-activating enzyme, putative
Leishmania infantum LinJ.24.0470   ubiquitin-activating enzyme, putative
Leishmania major LmjF.24.0460   ubiquitin-activating enzyme, putative
Leishmania mexicana LmxM.24.0460   ubiquitin-activating enzyme, putative
Mycobacterium tuberculosis Rv2338c   Possible molybdopterin biosynthesis protein MoeW
Oryza sativa 4340655   Os06g0251000
Saccharomyces cerevisiae YKL027W   Tcd2p
Trypanosoma brucei gambiense Tbg972.11.5840   ubiquitin activating enzyme, putative
Trypanosoma brucei Tb927.11.5170   ubiquitin activating enzyme, putative
Trypanosoma congolense TcIL3000_0_21730   ubiquitin activating enzyme, putative
Trypanosoma congolense TcIL3000.11.5380   ubiquitin activating enzyme, putative
Trypanosoma cruzi TcCLB.504131.30   ubiquitin-activating enzyme, putative
Trichomonas vaginalis TVAG_071600   ubiquitin-activating enzyme E1, putative

Essentiality

Rv2338c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2379 this record Mycobacterium tuberculosis essential nmpdr
Tb11.02.2830 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.02.2830 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.02.2830 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.02.2830 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2812 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Rv2338c (Mycobacterium tuberculosis), Possible molybdopterin biosynthesis protein MoeW
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