Detailed view for Rv2373c

Basic information

TDR Targets ID: 981910
Mycobacterium tuberculosis, Probable chaperone protein DnaJ2
Source Database / ID:  Tuberculist 

pI: 6.6485 | Length (AA): 382 | MW (Da): 40457 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00226  
PF00684  
PF01556  

Gene Ontology

Mouse over links to read term descriptions.
GO:0051082   unfolded protein binding  
GO:0031072   heat shock protein binding  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 61 5nro (B) 4 63 56.00 0 1 0.98755 -2.12
3 350 4j80 (A) 5 265 41.00 0 1 0.951295 0.99
3 68 5vso (A) 10 75 56.00 0 1 1.00887 -1.98
4 68 2och (A) 6 68 62.00 0 1 0.989257 -1.53
113 336 1nlt (A) 111 336 26.00 0 1 0.861487 0.36

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126952)

Species Accession Gene Product
Arabidopsis thaliana AT5G48030   gametophytic factor 2
Arabidopsis thaliana AT1G80030   molecular chaperone Hsp40/DnaJ-like protein
Arabidopsis thaliana AT4G39960   molecular chaperone Hsp40/DnaJ family protein
Arabidopsis thaliana AT2G22360   molecular chaperone DnaJ
Babesia bovis BBOV_III010470   dnaJ C terminal region domain containing protein
Brugia malayi Bm1_44845   DnaJ C terminal region family protein
Candida albicans CaO19.6672   DnaJ-like protein similar to S. cerevisiae MDJ1 (YFL016C) mitochondrial co-chaperone
Caenorhabditis elegans CELE_F22B7.5   Protein DNJ-10, isoform A
Cryptosporidium hominis Chro.20145   Pfj1
Cryptosporidium parvum cgd2_1330   DNAJ protein
Chlamydia trachomatis CT_341   heat shock protein DnaJ
Dictyostelium discoideum DDB_G0278789   heat shock protein DnaJ family protein
Drosophila melanogaster Dmel_CG5504   lethal (2) tumorous imaginal discs
Escherichia coli b0015   chaperone Hsp40, DnaK co-chaperone
Echinococcus granulosus EgrG_001009900   protein tumorous imaginal discs
Entamoeba histolytica EHI_022950   DnaJ family protein
Echinococcus multilocularis EmuJ_001009900   protein tumorous imaginal discs
Homo sapiens ENSG00000103423   DnaJ (Hsp40) homolog, subfamily A, member 3
Leishmania braziliensis LbrM.04.0730   chaperone protein DNAj, putative
Leishmania donovani LdBPK_040940.1   chaperone protein DnaJ, putative
Leishmania infantum LinJ.04.0940   chaperone protein DNAj, putative
Leishmania major LmjF.04.0940   chaperone protein DNAj, putative
Leishmania mexicana LmxM.04.0940  
Loa Loa (eye worm) LOAG_12869   DnaJ C terminal region family protein
Mycobacterium leprae ML2494c   PROBABLE CHAPERONE PROTEIN DNAJ1
Mycobacterium leprae ML0625   Probable chaperone protein DnaJ2
Mus musculus ENSMUSG00000004069   DnaJ (Hsp40) homolog, subfamily A, member 3
Mycobacterium tuberculosis Rv2373c   Probable chaperone protein DnaJ2
Mycobacterium tuberculosis Rv0352   Probable chaperone protein DnaJ1
Mycobacterium ulcerans MUL_3626   chaperone protein DnaJ
Mycobacterium ulcerans MUL_0595   chaperone protein DnaJ
Neospora caninum NCLIV_002690   Chaperone protein dnaJ, related
Oryza sativa 4338452   Os05g0334400
Oryza sativa 4339933   Os06g0116800
Oryza sativa 4338449   Os05g0333500
Oryza sativa 4351608   Os12g0168400
Oryza sativa 4331058   Os02g0804500
Plasmodium berghei PBANKA_1007000   chaperone protein DnaJ, putative
Plasmodium falciparum PF3D7_0409400   chaperone protein DnaJ
Plasmodium knowlesi PKNH_0307500   chaperone protein DnaJ, putative
Plasmodium vivax PVX_000775   chaperone DNAJ protein, putative
Plasmodium yoelii PY04093   Pfj1
Saccharomyces cerevisiae YFL016C   Mdj1p
Schistosoma japonicum Sjp_0207660   ko:K09504 DnaJ homolog, subfamily A, member 3, putative
Schistosoma japonicum Sjp_0046950   DnaJ homolog subfamily A member 3, mitochondrial precursor, putative
Schistosoma mansoni Smp_096010   DNAj homolog subfamily A member
Schmidtea mediterranea mk4.000318.03  
Schmidtea mediterranea mk4.004989.03   DnaJ homolog dnj-10
Schmidtea mediterranea mk4.000477.18   DnaJ homolog subfamily A member 3, mitochondrial
Schmidtea mediterranea mk4.001157.00  
Trypanosoma brucei gambiense Tbg972.9.4590   chaperone protein DNAj, putative
Trypanosoma brucei Tb427tmp.211.0330   chaperone protein DNAj, putative
Trypanosoma brucei Tb927.9.8410   chaperone protein DnaJ, putative
Trypanosoma congolense TcIL3000_9_2900   chaperone protein DnaJ, putative
Trypanosoma cruzi TcCLB.510243.30   chaperone DNAJ protein, putative
Trypanosoma cruzi TcCLB.510575.200   chaperone DNAJ protein, putative
Toxoplasma gondii TGME49_207760   DnaJ C terminal region domain-containing protein
Treponema pallidum TP0217   heat shock protein
Theileria parva TP02_0096   chaperone protein dnaJ, putative
Trichomonas vaginalis TVAG_019190   chaperone protein DNAj, putative
Trichomonas vaginalis TVAG_165050   chaperone protein DNAj, putative
Wolbachia endosymbiont of Brugia malayi Wbm0785   molecular chaperone DnaJ

Essentiality

Rv2373c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu355 Mycobacterium tuberculosis essential nmpdr
Tb09.211.0330 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.0330 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.0330 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb09.211.0330 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0015 Escherichia coli non-essential goodall
CELE_F22B7.5 Caenorhabditis elegans embryonic lethal wormbase
CELE_F22B7.5 Caenorhabditis elegans larval arrest wormbase
PBANKA_1007000 Plasmodium berghei Essential plasmo
TGME49_207760 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2373c (Mycobacterium tuberculosis), Probable chaperone protein DnaJ2
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