Detailed view for Rv2441c

Basic information

TDR Targets ID: 7056
Mycobacterium tuberculosis, 50S ribosomal protein L27 RpmA
Source Database / ID:  Tuberculist 

pI: 12.066 | Length (AA): 86 | MW (Da): 8968 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01016   Ribosomal L27 protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0005840   ribosome  
GO:0005622   intracellular  
GO:0003735   structural constituent of ribosome  
GO:0006412   translation  

Metabolic Pathways

Ribosome (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 83 5dm6 (T) 2 84 60.00 0 1 1.62339 -0.19
20 82 1v8q (A) 20 83 65.00 0.000000000013 1 1.49346 -0.9

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_127361)

Species Accession Gene Product
Arabidopsis thaliana AT2G16930   ribosomal protein L27 family protein
Arabidopsis thaliana AT5G15220   large subunit ribosomal protein L27
Arabidopsis thaliana AT5G40950   50S ribosomal protein L27
Babesia bovis BBOV_IV007200   ribosomal protein L27, putative
Candida albicans CaO19_7203   hypothetical protein
Candida albicans CaO19.7203   likely mitochondrial ribosomal protein similar to S. cerevisiae MRP7 (YNL005C) large subunit protein (E. coli L27)
Chlamydia trachomatis CT_419   50S ribosomal protein L27
Dictyostelium discoideum DDB_G0278889   ribosomal protein L27, mitochondrial
Drosophila melanogaster Dmel_CG33002   mitochondrial ribosomal protein L27
Escherichia coli b3185   50S ribosomal subunit protein L27
Echinococcus granulosus EgrG_000696000   Mitochondrial 39S ribosomal protein L27 L27mt
Echinococcus multilocularis EmuJ_000696000   Mitochondrial 39S ribosomal protein L27 (L27mt)
Homo sapiens ENSG00000108826   mitochondrial ribosomal protein L27
Mycobacterium leprae ML1466c   PROBABLE 50S RIBOSOMAL PROTEIN L27 RPMA
Mus musculus ENSMUSG00000024414   mitochondrial ribosomal protein L27
Mycobacterium tuberculosis Rv2441c   50S ribosomal protein L27 RpmA
Mycobacterium ulcerans MUL_3713   50S ribosomal protein L27
Neospora caninum NCLIV_000540   50S ribosomal protein L27, chloroplastic, related
Oryza sativa 9271228   Os08g0404250
Oryza sativa 4327257   Os01g0924000
Oryza sativa 4345523   Os08g0404300
Plasmodium berghei PBANKA_0518000   apicoplast ribosomal protein L27 precursor, putative
Plasmodium falciparum PF3D7_1034200   apicoplast ribosomal protein L27 precursor, putative
Plasmodium knowlesi PKNH_0619400   apicoplast ribosomal protein L27 precursor, putative
Plasmodium vivax PVX_111015   50S ribosomal protein L27, putative
Plasmodium yoelii PY00465   ribosomal protein L27, putative
Saccharomyces cerevisiae YNL005C   mitochondrial 54S ribosomal protein YmL2
Schistosoma japonicum Sjp_0313340   expressed protein
Schistosoma mansoni Smp_037120   Mitochondrial 39S ribosomal protein L27 (L27mt) (MRP-L27)
Schmidtea mediterranea mk4.045079.04  
Schmidtea mediterranea mk4.073758.00  
Schmidtea mediterranea mk4.000818.07   Putative mitochondrial 39S ribosomal protein L27
Toxoplasma gondii TGME49_293600   ribosomal protein RPL27
Treponema pallidum TP0743   50S ribosomal protein L27
Theileria parva TP03_0228   60S ribosomal protein L27, putative
Wolbachia endosymbiont of Brugia malayi Wbm0700   50S ribosomal protein L27

Essentiality

Rv2441c has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2484 this record Mycobacterium tuberculosis essential nmpdr
b3185 Escherichia coli essential goodall
TGME49_293600 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2441c (Mycobacterium tuberculosis), 50S ribosomal protein L27 RpmA
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