Detailed view for Rv2674

Basic information

TDR Targets ID: 8693
Mycobacterium tuberculosis, Probable peptide methionine sulfoxide reductase MsrB (protein-methionine-R-oxide reductase) (peptide met(O) reductase)
Source Database / ID:  Tuberculist 

pI: 6.622 | Length (AA): 136 | MW (Da): 15318 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01641   SelR domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0033743   peptide-methionine (R)-S-oxide reductase activity  
GO:0030091   protein repair  
GO:0016671   oxidoreductase activity, acting on sulfur group of donors, disulfide as acceptor  
GO:0055114   oxidation reduction  
GO:0006979   response to oxidative stress  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 133 3e0m (A) 163 294 39.00 0 1 1.54774 -1.05
4 132 3cxk (A) 10 137 48.00 0 1 1.62923 -1.1
5 133 2k8d (A) 23 149 58.00 0 1 1.60833 0.05
7 133 3hcg (A) 380 505 41.00 0 1 1.55952 -1.25

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase. murphy
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Dormant phase. hasan
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_127019)

Species Accession Gene Product
Arabidopsis thaliana AT4G21830   peptide methionine sulfoxide reductase B7
Arabidopsis thaliana AT4G04800   peptide methionine sulfoxide reductase B3
Arabidopsis thaliana AT4G04810   peptide methionine sulfoxide reductase B4
Arabidopsis thaliana AT4G21840   methionine sulfoxide reductase B8
Arabidopsis thaliana AT4G21860   peptide methionine sulfoxide reductase B2
Arabidopsis thaliana AT4G04830   peptide methionine sulfoxide reductase B5
Arabidopsis thaliana AT1G53670   peptide methionine sulfoxide reductase B1
Arabidopsis thaliana AT4G21850   methionine sulfoxide reductase B9
Arabidopsis thaliana AT4G04840   methionine sulfoxide reductase B6
Brugia malayi Bm1_44650   peptide methionine sulfoxide reductase msrB
Candida albicans CaO19.3292   similar to S. cerevisiae orf YCL033C
Candida albicans CaO19.10802   similar to S. cerevisiae orf YCL033C
Caenorhabditis elegans CELE_F44E2.6   Protein F44E2.6, isoform B
Dictyostelium discoideum DDB_G0291145   hypothetical protein
Drosophila melanogaster Dmel_CG6584   Selenoprotein R
Escherichia coli b1778   methionine sulfoxide reductase B
Echinococcus granulosus EgrG_000840900   peptide methionine sulfoxide reductase
Echinococcus multilocularis EmuJ_000840900   peptide methionine sulfoxide reductase
Giardia lamblia GL50803_5180   Peptide methionine sulfoxide reductase msrB
Homo sapiens ENSG00000174099   methionine sulfoxide reductase B3
Homo sapiens ENSG00000148450   methionine sulfoxide reductase B2
Leishmania braziliensis LbrM.28.2870   hypothetical protein, conserved
Leishmania donovani LdBPK_282860.1   SelR domain containing protein, putative
Leishmania infantum LinJ.28.2860   hypothetical protein, conserved
Leishmania major LmjF.28.2660   hypothetical protein, conserved
Leishmania mexicana LmxM.28.2660   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_00565   peptide methionine sulfoxide reductase msrB
Mus musculus ENSMUSG00000051236   methionine sulfoxide reductase B3
Mus musculus ENSMUSG00000023094   methionine sulfoxide reductase B2
Mycobacterium tuberculosis Rv2674   Probable peptide methionine sulfoxide reductase MsrB (protein-methionine-R-oxide reductase) (peptide met(O) reductase)
Mycobacterium ulcerans MUL_3308   hypothetical protein
Neospora caninum NCLIV_057200   AT4G21860 protein, related
Oryza sativa 4341018   Os06g0472000
Oryza sativa 4338744   Os05g0404200
Oryza sativa 4332888   Os03g0360700
Saccharomyces cerevisiae YCL033C   Mxr2p
Schistosoma japonicum Sjp_0306390   ko:K07304 peptide-methionine (S)-S-oxide reductase [EC1.8.4.11], putative
Schistosoma japonicum Sjp_0203880   ko:K07305 peptide-methionine (R)-S-oxide reductase [EC1.8.4.12], putative
Schistosoma mansoni Smp_167890.1   methionine sulfoxide reductase B2b
Schistosoma mansoni Smp_143340   methionine sulfoxide reductase
Schistosoma mansoni Smp_167890.2   methionine sulfoxide reductase B2b
Schmidtea mediterranea mk4.014965.00   Methionine-R-sulfoxide reductase B2, mitochondrial
Trypanosoma brucei gambiense Tbg972.11.13390   hypothetical protein, conserved
Trypanosoma brucei Tb927.11.11930   SelR domain containing protein, putative
Trypanosoma congolense TcIL3000.11.12590   SelR domain containing protein, putative
Trypanosoma cruzi TcCLB.511211.49   hypothetical protein, conserved
Toxoplasma gondii TGME49_314000   peptide methionine sulfoxide reductase msrB, putative
Trichomonas vaginalis TVAG_156810   methionine sulfoxide reductase, putative

Essentiality

Rv2674 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu2720 this record Mycobacterium tuberculosis non-essential nmpdr
Tb11.01.3760 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.3760 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.3760 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.01.3760 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_314000 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Rv2674 (Mycobacterium tuberculosis), Probable peptide methionine sulfoxide reductase MsrB (protein-methionine-R-oxide reductase) (peptide met(O) reductase)
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