Detailed view for Rv2727c

Basic information

TDR Targets ID: 980793
Mycobacterium tuberculosis, Probable tRNA delta(2)-isopentenylpyrophosphate transferase MiaA (IPP transferase) (isopentenyl-diphosphate:tRNA isopentenyltran
Source Database / ID:  Tuberculist 

pI: 10.2144 | Length (AA): 314 | MW (Da): 34414 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 305 3d3q (A) 8 306 31.00 0 1 1.29265 -0.03
2 310 3a8t (A) 31 311 26.00 0 1 0.954576 1.19
2 307 3exa (A) 4 305 36.00 0 1 1.31102 0.06

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127078)

Species Accession Gene Product
Arabidopsis thaliana AT2G27760   tRNA isopentenyltransferase 2
Arabidopsis thaliana AT5G20040   tRNA isopentenyltransferase AtIPT9
Babesia bovis BBOV_IV006320   hypothetical protein
Babesia bovis BBOV_IV006330   hypothetical protein
Brugia malayi Bm1_47380   Abnormal growth rate protein 1
Candida albicans CaO19.4801   likely tRNA isopentenyltransferase similar to S. cerevisiae MOD5 (YOR274W) tRNA modification enzyme that competes with Erg20p fo
Candida albicans CaO19.12264   likely tRNA isopentenyltransferase similar to S. cerevisiae MOD5 (YOR274W) tRNA modification enzyme that competes with Erg20p fo
Caenorhabditis elegans CELE_ZC395.6   Protein GRO-1
Cryptosporidium hominis Chro.60293   tRNA delta(2)-isopentenylpyrophosphate transferase
Cryptosporidium parvum cgd6_2540   tRNA delta(2)-isopentenylpyrophosphate transferase, putative
Chlamydia trachomatis CT_766   tRNA dimethylallyltransferase
Dictyostelium discoideum DDB_G0291528   hypothetical protein
Dictyostelium discoideum DDB_G0273039   hypothetical protein
Dictyostelium discoideum DDB_G0274017   hypothetical protein
Drosophila melanogaster Dmel_CG31381   CG31381 gene product from transcript CG31381-RA
Escherichia coli b4171   delta(2)-isopentenylpyrophosphate tRNA-adenosine transferase
Echinococcus granulosus EgrG_000997300   tRNA dimethylallyltransferase
Entamoeba histolytica EHI_155640   tRNA isopentenyltransferase, putative
Echinococcus multilocularis EmuJ_000997300   tRNA dimethylallyltransferase
Homo sapiens ENSG00000043514   tRNA isopentenyltransferase 1
Leishmania braziliensis LbrM.29.0970   hypothetical protein, conserved
Leishmania donovani LdBPK_290980.1   IPP transferase, putative
Leishmania infantum LinJ.29.0980   hypothetical protein, conserved
Leishmania major LmjF.29.0900   hypothetical protein, conserved
Leishmania mexicana LmxM.08_29.0900   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_02994   abnormal growth rate protein 1
Mycobacterium leprae ML0995   PROBABLE TRNA DELTA(2)-ISOPENTENYLPYROPHOSPHATE TRANSFERASE MIAA (IPP TRANSFERASE) (ISOPENTENYL-DIPHOSPHATE:TRNA ISOPENTENYLTRAN
Mus musculus ENSMUSG00000028653   tRNA isopentenyltransferase 1
Mycobacterium tuberculosis Rv2727c   Probable tRNA delta(2)-isopentenylpyrophosphate transferase MiaA (IPP transferase) (isopentenyl-diphosphate:tRNA isopentenyltran
Mycobacterium ulcerans MUL_3469   tRNA delta(2)-isopentenylpyrophosphate transferase MiaA
Mycobacterium ulcerans MUL_3370   tRNA delta(2)-isopentenylpyrophosphate transferase
Neospora caninum NCLIV_056050   hypothetical protein
Oryza sativa 4342142   Os06g0729800
Plasmodium berghei PBANKA_0606100   tRNA delta(2)-isopentenylpyrophosphate transferase, putative
Plasmodium falciparum PF3D7_1207600   tRNA delta(2)-isopentenylpyrophosphate transferase, putative
Plasmodium knowlesi PKNH_1307500   tRNA delta(2)-isopentenylpyrophosphate transferase, putative
Plasmodium vivax PVX_084417   tRNA delta(2)-isopentenylpyrophosphate transferase, putative
Plasmodium yoelii PY04164   tRNA delta(2)-isopentenylpyrophosphate transferase, putative
Saccharomyces cerevisiae YOR274W   Mod5p
Schistosoma japonicum Sjp_0050290   ko:K00791 tRNA delta(2)-isopentenylpyrophosphate transferase [EC2.5.1.8], putative
Schistosoma japonicum Sjp_0208980   ko:K00791 tRNA delta(2)-isopentenylpyrophosphate transferase [EC2.5.1.8], putative
Schistosoma mansoni Smp_102920   tRNA delta(2)-isopentenylpyrophosphate transferase
Schistosoma mansoni Smp_118990   tRNA delta(2)-isopentenylpyrophosphate transferase-related
Schmidtea mediterranea mk4.000216.16   tRNA dimethylallyltransferase, mitochondrial
Trypanosoma brucei gambiense Tbg972.3.5490   tRNA isopentenyltransferase, putative
Trypanosoma brucei Tb927.3.4900   tRNA isopentenyltransferase, putative
Trypanosoma congolense TcIL3000_3_2950   tRNA isopentenyltransferase, putative
Trypanosoma cruzi TcCLB.507951.250   tRNA isopentenyltransferase, putative
Toxoplasma gondii TGME49_312520   tRNA dimethylallyltransferase
Toxoplasma gondii TGME49_312510   Ku70/Ku80 beta-barrel domain-containing protein
Treponema pallidum TP0637   tRNA delta(2)-isopentenylpyrophosphate transferase
Theileria parva TP01_0445   tRNA delta(2)-isopentenylpyrophosphate transferase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0636   tRNA delta(2)-isopentenylpyrophosphate transferase

Essentiality

Rv2727c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.4900 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.4900 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.4900 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.4900 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b4171 Escherichia coli non-essential goodall
CELE_ZC395.6 Caenorhabditis elegans larval arrest wormbase
PBANKA_0606100 Plasmodium berghei Dispensable plasmo
TGME49_312510 Toxoplasma gondii Essentiality uncertain sidik
TGME49_312520 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2727c (Mycobacterium tuberculosis), Probable tRNA delta(2)-isopentenylpyrophosphate transferase MiaA (IPP transferase) (isopentenyl-diphosphate:tRNA isopentenyltran
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