Detailed view for Rv2966c

Basic information

TDR Targets ID: 981307
Mycobacterium tuberculosis, Possible methyltransferase (methylase)
Source Database / ID:  Tuberculist 

pI: 9.0445 | Length (AA): 188 | MW (Da): 19789 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF03602  

Gene Ontology

Mouse over links to read term descriptions.
GO:0031167   rRNA methylation  
GO:0008168   methyltransferase activity  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 184 3p9n (A) 1 184 99.00 0 1 2.21922 -1.7
3 122 2fhp (A) 2 123 42.00 0.00000000024 1 1.0458 0.03

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_130478)

Species Accession Gene Product
Arabidopsis thaliana AT3G28460   methyltransferase
Babesia bovis BBOV_IV007610   conserved hypothetical portein
Chlamydia trachomatis CT_487   methylase
Escherichia coli b3465   16S rRNA m(2)G966 methyltransferase, SAM-dependent
Mycobacterium leprae ML1664c   POSSIBLE METHYLTRANSFERASE (METHYLASE)
Mycobacterium tuberculosis Rv2966c   Possible methyltransferase (methylase)
Neospora caninum NCLIV_035060   hypothetical protein, conserved
Oryza sativa 4334030   Os03g0736400
Plasmodium berghei PBANKA_1363500   N6-adenine-specific methylase, putative
Plasmodium falciparum PF3D7_1350700   N6-adenine-specific methylase, putative
Plasmodium knowlesi PKNH_1250200   N6-adenine-specific methylase, putative
Plasmodium vivax PVX_083430   hypothetical protein, conserved
Plasmodium yoelii PY06977   similar to unknown proteins
Toxoplasma gondii TGME49_272210   hypothetical protein
Treponema pallidum TP0065   hypothetical protein
Theileria parva TP01_0343   hypothetical protein
Wolbachia endosymbiont of Brugia malayi Wbm0791   N-6 adenine-specific DNA methylase

Essentiality

Rv2966c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
b3465 Escherichia coli non-essential goodall
TGME49_272210 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv2966c (Mycobacterium tuberculosis), Possible methyltransferase (methylase)
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