Detailed view for Rv3024c

Basic information

TDR Targets ID: 981709
Mycobacterium tuberculosis, Probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU
Source Database / ID:  Tuberculist 

pI: 8.0122 | Length (AA): 367 | MW (Da): 38120 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Sulfur relay system (KEGG)

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 356 2hma (A) 8 363 36.00 0 1 1.3872 -0.3
2 359 2der (A) 7 366 31.00 0 1 1.20148 0.54
2 77 2ywb (A) 211 274 45.00 0.072 0.15 0.389984 0.92

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_127383)

Species Accession Gene Product
Arabidopsis thaliana AT1G51310   tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
Babesia bovis BBOV_III005960   tRNA methyl transferase family protein
Brugia malayi Bm1_08740   tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase
Candida albicans CaO19.7245   similar to S. cerevisiae YDL033C
Caenorhabditis elegans CELE_B0035.16   Protein B0035.16
Chlamydia trachomatis CT_287   tRNA-specific 2-thiouridylase MnmA
Dictyostelium discoideum DDB_G0288979   tRNA -methyltransferase
Drosophila melanogaster Dmel_CG3021   CG3021 gene product from transcript CG3021-RA
Escherichia coli b1133   tRNA(Gln,Lys,Glu) U34 2-thiouridylase, first step in mnm(5)-s(2)U34-tRNA synthesis
Echinococcus granulosus EgrG_001192300   mitochondrial tRNA specific 2 thiouridylase
Echinococcus multilocularis EmuJ_001192300   mitochondrial tRNA specific 2 thiouridylase
Homo sapiens ENSG00000100416   tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
Leishmania braziliensis LbrM.07.0130   tRNA-methyl transferase, putative
Leishmania donovani LdBPK_070280.1   tRNA-methyl transferase, putative
Leishmania infantum LinJ.07.0280   tRNA-methyl transferase, putative
Leishmania major LmjF.07.0130   tRNA-methyl transferase, putative
Leishmania mexicana LmxM.07.0130   tRNA-methyl transferase, putative
Loa Loa (eye worm) LOAG_02754   hypothetical protein
Mycobacterium leprae ML1707c   probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU
Mus musculus ENSMUSG00000022386   tRNA 5-methylaminomethyl-2-thiouridylate methyltransferase
Mycobacterium tuberculosis Rv3024c   Probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU
Mycobacterium ulcerans MUL_1928   tRNA-specific 2-thiouridylase MnmA
Neospora caninum NCLIV_053570   tRNA methyltransferase domain-containing protein, putative
Oryza sativa 4331793   Os03g0174600
Plasmodium berghei PBANKA_0504000   tRNA methyltransferase, putative
Plasmodium falciparum PF3D7_1019800   tRNA methyltransferase, putative
Plasmodium knowlesi PKNH_0604000   tRNA methyltransferase, putative
Plasmodium vivax PVX_001855   tRNA methyltransferase, putative
Plasmodium yoelii PY00607   probable tRNA-methyltransferase
Saccharomyces cerevisiae YDL033C   Slm3p
Schistosoma japonicum Sjp_0015200   ko:K00566 tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase [EC2.1.1.61], putative
Schistosoma mansoni Smp_113390   tRNA(5-methylaminomethyl-2-thiouridylate)-methy lt ransferase
Schistosoma mansoni Smp_178920   tRNA(5-methylaminomethyl-2-thiouridylate)-methy lt ransferase
Schmidtea mediterranea mk4.015951.00   Probable mitochondrial tRNA-specific 2-thiouridylase 1
Schmidtea mediterranea mk4.066048.00  
Schmidtea mediterranea mk4.034030.00   Probable mitochondrial tRNA-specific 2-thiouridylase 1
Schmidtea mediterranea mk4.040827.00  
Trypanosoma brucei gambiense Tbg972.8.1460   tRNA-methyl transferase, putative
Trypanosoma brucei Tb927.8.1830   tRNA-methyl transferase, putative
Trypanosoma congolense TcIL3000_8_1760   tRNA-methyl transferase, putative
Toxoplasma gondii TGME49_309110   tRNA methyl transferase
Theileria parva TP02_0339   tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0155   tRNA-specific 2-thiouridylase MnmA

Essentiality

Rv3024c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.1830 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.1830 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.1830 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.1830 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b1133 Escherichia coli essential goodall
PBANKA_0504000 Plasmodium berghei Essential plasmo
TGME49_309110 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Rv3024c (Mycobacterium tuberculosis), Probable tRNA (5-methylaminomethyl-2-thiouridylate)-methyltransferase TrmU
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