Detailed view for Rv3110

Basic information

TDR Targets ID: 6498
Mycobacterium tuberculosis, Probable pterin-4-alpha-carbinolamine dehydratase MoaB1 (PHS) (4-alpha- hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbin
Source Database / ID:  Tuberculist 

pI: 5.9744 | Length (AA): 131 | MW (Da): 14558 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01329   Pterin 4 alpha carbinolamine dehydratase

Gene Ontology

Mouse over links to read term descriptions.
GO:0008124   4-alpha-hydroxytetrahydrobiopterin dehydratase activity  
GO:0006729   tetrahydrobiopterin biosynthetic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
32 122 2ebb (A) 5 95 31.00 0 1 1.19656 -1.34
32 121 2ebb (A) 5 94 34.00 0.0000000019 0.99 1.16592 -0.91

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_127905)

Species Accession Gene Product
Arabidopsis thaliana AT1G29810   transcriptional coactivator/pterin dehydratase
Brugia malayi Bm1_48890   Pterin-4-alpha-carbinolamine dehydratase
Candida albicans CaO19.9626   similar to pterin-4a-carbinolamine dehydratase
Candida albicans CaO19.2079   similar to pterin-4a-carbinolamine dehydratase
Caenorhabditis elegans CELE_T10B11.1   Protein PCBD-1
Dictyostelium discoideum DDB_G0282831   pterin-4-alpha-carbinolamine dehydratase
Dictyostelium discoideum DDB_G0278993   hypothetical protein
Drosophila melanogaster Dmel_CG1963   pterin-4a-carbinolamine dehydratase
Homo sapiens 84105   pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 2
Homo sapiens ENSG00000166228   pterin-4 alpha-carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha
Leishmania braziliensis LbrM.19.0100   pterin-4-alpha-carbinolamine dehyddrogenase
Leishmania donovani LdBPK_110220.1   pterin-4-alpha-carbinolamine dehydratase, putative
Leishmania infantum LinJ.11.0220   pterin-4-alpha-carbinolamine dehydratase, putative
Leishmania major LmjF.11.0220   pterin-4-alpha-carbinolamine dehydratase, putative
Leishmania mexicana LmxM.11.0220   pterin-4-alpha-carbinolamine dehydratase, putative
Loa Loa (eye worm) LOAG_13837   pterin-4-alpha-carbinolamine dehydratase
Mycobacterium leprae ML1503A   conserved hypothetical protein
Mus musculus ENSMUSG00000021496   pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 2
Mus musculus ENSMUSG00000020098   pterin 4 alpha carbinolamine dehydratase/dimerization cofactor of hepatocyte nuclear factor 1 alpha (TCF1) 1
Mycobacterium tuberculosis Rv3110   Probable pterin-4-alpha-carbinolamine dehydratase MoaB1 (PHS) (4-alpha- hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbin
Mycobacterium tuberculosis Rv1159A   Unknown protein
Mycobacterium ulcerans MUL_1007   pterin-4-alpha-carbinolamine dehydratase
Neospora caninum NCLIV_046820   pterin-4a-carbinolamine dehydratase, putative
Oryza sativa 4327862   Os01g0663500
Plasmodium falciparum PF3D7_1108300   pterin-4a-carbinolamine dehydratase
Plasmodium knowlesi PKNH_0905900   pterin-4a-carbinolamine dehydratase, putative
Plasmodium vivax PVX_091092   pterin-4a-carbinolamine dehydratase, putative
Saccharomyces cerevisiae YHL018W   4A-hydroxytetrahydrobiopterin dehydratase
Schistosoma japonicum Sjp_0307980   ko:K01724 Pterin CarBinolamine Dehydratase [EC:4.2.1.96], putative
Schistosoma mansoni Smp_016670   4a-hydroxytetrahydrobiopterin dehydratase
Schmidtea mediterranea mk4.000506.00   Putative pterin-4-alpha-carbinolamine dehydratase
Trypanosoma brucei gambiense Tbg972.11.7970   pterin-4-alpha-carbinolamine dehydratase, putative
Trypanosoma brucei Tb927.11.7040   pterin-4-alpha-carbinolamine dehydratase, putative
Trypanosoma cruzi TcCLB.503613.40   pterin-4-alpha-carbinolamine dehydratase, putative
Trypanosoma cruzi TcCLB.511165.54   pterin-4-alpha-carbinolamine dehydratase, putative
Toxoplasma gondii TGME49_226010   pterin-4-alpha-carbinolamine dehydratase

Essentiality

Rv3110 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3163 this record Mycobacterium tuberculosis non-essential nmpdr
Tb11.02.4890 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb11.02.4890 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.02.4890 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.02.4890 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_226010 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier Rv3110 (Mycobacterium tuberculosis), Probable pterin-4-alpha-carbinolamine dehydratase MoaB1 (PHS) (4-alpha- hydroxy-tetrahydropterin dehydratase) (pterin-4-a-carbin
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