Detailed view for Rv3282

Basic information

TDR Targets ID: 9046
Mycobacterium tuberculosis, Conserved hypothetical protein
Source Database / ID:  Tuberculist 

pI: 6.2278 | Length (AA): 222 | MW (Da): 23010 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02545   Maf-like protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0047429   nucleoside-triphosphate diphosphatase activity  
GO:0005737   cytoplasm  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 205 4oo0 (A) 17 214 26.00 0 1 1.25981 -0.44
3 205 4jhc (A) 3 190 28.00 0 1 1.21481 -0.34
3 203 2p5x (A) 14 212 31.00 0 1 1.28781 -0.36

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.

Orthologs

Ortholog group members (OG5_127554)

Species Accession Gene Product
Babesia bovis BBOV_III007990   Maf-like protein family protein
Brugia malayi Bm1_26810   maf protein
Candida albicans CaO19.7051   similar to S. cerevisiae YOR111W
Candida albicans CaO19_7051   hypothetical protein
Caenorhabditis elegans CELE_C54G4.6   Protein DOD-18
Chlamydia trachomatis CT_349   Maf protein
Dictyostelium discoideum DDB_G0267852   hypothetical protein
Drosophila melanogaster Dmel_CG9515   CG9515 gene product from transcript CG9515-RB
Escherichia coli b3248   Maf-like protein
Echinococcus granulosus EgrG_001023400   maf protein
Echinococcus granulosus EgrG_001023500   maf protein
Entamoeba histolytica EHI_146200   septum formation protein maf, putative
Echinococcus multilocularis EmuJ_001023500   maf protein
Echinococcus multilocularis EmuJ_001023400   maf protein
Giardia lamblia GL50803_13889   Maf-like protein yhdE
Homo sapiens ENSG00000169093   acetylserotonin O-methyltransferase-like
Loa Loa (eye worm) LOAG_04181   hypothetical protein
Mycobacterium leprae ML0729c   Conserved hypothetical protein
Mus musculus 69099   RIKEN cDNA 1810009N02 gene
Mycobacterium tuberculosis Rv3282   Conserved hypothetical protein
Mycobacterium ulcerans MUL_2634   Maf-like protein
Neospora caninum NCLIV_011970   septum formation protein maf, putative
Oryza sativa 4333958   Os03g0724700
Onchocerca volvulus OVOC13398  
Plasmodium berghei PBANKA_1412100   Maf-like protein, putative
Plasmodium falciparum PF3D7_1313700   Maf-like protein, putative
Plasmodium knowlesi PKNH_1414300   Maf-like protein, putative
Plasmodium vivax PVX_122505   septum formation protein Maf domain containing protein
Plasmodium yoelii PY02834   maf protein
Saccharomyces cerevisiae YOR111W   hypothetical protein
Schistosoma japonicum Sjp_0103960   ko:K06287 septum formation protein, putative
Schistosoma mansoni Smp_061040   maf protein
Schmidtea mediterranea mk4.000952.00  
Trypanosoma brucei gambiense Tbg972.1.2030   septum formation protein MAF homologue, putative
Trypanosoma brucei Tb927.1.3280   septum formation protein MAF homologue, putative
Trypanosoma congolense TcIL3000_0_45370   septum formation protein MAF homologue, putative
Trypanosoma cruzi TcCLB.509819.30   septum formation inhibitor, Maf-like protein, putative
Trypanosoma cruzi TcCLB.503663.10   septum formation inhibitor, Maf-like protein, putative
Toxoplasma gondii TGME49_301430   septum formation protein maf, putative
Theileria parva TP04_0705   septum formation protein MAF, putative
Trichomonas vaginalis TVAG_160900   O-methyltransferase, putative
Trichomonas vaginalis TVAG_265960   O-methyltransferase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0192   Maf-like protein

Essentiality

Rv3282 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3341 this record Mycobacterium tuberculosis non-essential nmpdr
Tb927.1.3280 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.3280 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.1.3280 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.3280 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3248 Escherichia coli non-essential goodall
TGME49_301430 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv3282 (Mycobacterium tuberculosis), Conserved hypothetical protein
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