Detailed view for Rv3284

Basic information

TDR Targets ID: 9047
Mycobacterium tuberculosis, Conserved hypothetical protein
Source Database / ID:  Tuberculist 

pI: 4.5117 | Length (AA): 143 | MW (Da): 15395 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02657   Fe-S metabolism associated domain

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
3 141 4lw4 (C) 9 146 26.00 0 1 1.38293 -0.89
5 141 3g0m (A) 2 136 19.00 0 1 1.26974 -0.92
7 142 1wlo (A) 2 133 38.00 0 1 1.53005 -1.38

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase, Dormant phase. hasan murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_127283)

Species Accession Gene Product
Arabidopsis thaliana AT1G55805   BolA-like protein
Arabidopsis thaliana AT4G26500   chloroplast sulfur E
Brugia malayi Bm1_51155   BolA-like protein
Candida albicans CaO19.12483   BolA-like protein, similar to S. pombe Uvi31
Candida albicans CaO19.5016   BolA-like protein, similar to S. pombe Uvi31.
Caenorhabditis elegans CELE_K11H12.1   Protein K11H12.1
Chlamydia trachomatis CT_325   hypothetical protein
Dictyostelium discoideum DDB_G0274169   bolA family protein
Dictyostelium discoideum DDB_G0290319   bolA family protein
Drosophila melanogaster Dmel_CG31126   CG31126 gene product from transcript CG31126-RA
Escherichia coli b1679   sulfur acceptor protein
Escherichia coli b2811   CsdA-binding activator
Escherichia coli b0435   stationary-phase morphogene, transcriptional repressor for mreB
Echinococcus granulosus EgrG_001092600   bolA protein 1
Echinococcus multilocularis EmuJ_001092600   bolA protein 1
Homo sapiens ENSG00000178096   bolA family member 1
Leishmania braziliensis LbrM.33.0911   hypothetical protein, conserved
Leishmania donovani LdBPK_212230.1   BolA-like protein, putative
Leishmania infantum LinJ.21.2230   hypothetical protein, conserved
Leishmania major LmjF.21.1855   hypothetical protein, conserved
Leishmania mexicana LmxM.21.1855   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_16017   hypothetical protein
Loa Loa (eye worm) LOAG_07713   hypothetical protein
Mus musculus ENSMUSG00000015943   bolA-like 1 (E. coli)
Mycobacterium tuberculosis Rv3284   Conserved hypothetical protein
Mycobacterium ulcerans MUL_2636   Fe-S metabolism associated protein, SufE
Neospora caninum NCLIV_068570   Fe-S metabolism associated domain-containing protein, putative
Neospora caninum NCLIV_015980   hypothetical protein
Oryza sativa 4331656   Os03g0153400
Oryza sativa 4346577   Os09g0270900
Oryza sativa 4332085   Os03g0219500
Plasmodium berghei PBANKA_0303800   cysteine desulfuration protein SufE
Plasmodium falciparum PF3D7_0206100   cysteine desulfuration protein SufE
Plasmodium knowlesi PKNH_0414800   cysteine desulfuration protein SufE, putative
Plasmodium vivax PVX_003740   cysteine desulfuration protein SufE, putative
Plasmodium yoelii PY05037   sufE protein, putative
Saccharomyces cerevisiae YAL044W-A   hypothetical protein
Schistosoma japonicum Sjp_0210640   ko:K05527 BolA protein, putative
Schmidtea mediterranea mk4.008646.01   Putative bolA-like protein
Schmidtea mediterranea mk4.011214.00   Putative bolA-like protein
Trypanosoma brucei gambiense Tbg972.10.170   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.260   BolA-like protein, putative
Trypanosoma congolense TcIL3000_10_120   BolA-like protein, putative
Trypanosoma cruzi TcCLB.504069.34   BolA-like protein, putative
Trypanosoma cruzi TcCLB.511145.24   BolA-like protein, putative
Toxoplasma gondii TGME49_277010   Fe-S metabolism associated domain-containing protein
Toxoplasma gondii TGME49_239320   BolA family protein
Wolbachia endosymbiont of Brugia malayi Wbm0494   stress-induced morphogen, BolA

Essentiality

Rv3284 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3343 this record Mycobacterium tuberculosis non-essential nmpdr
Tb927.10.260 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.260 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.260 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.260 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0435 Escherichia coli non-essential goodall
b1679 Escherichia coli non-essential goodall
b2811 Escherichia coli non-essential goodall
PBANKA_0303800 Plasmodium berghei Essential plasmo
TGME49_277010 Toxoplasma gondii Probably non-essential sidik
TGME49_239320 Toxoplasma gondii Probably non-essential sidik
TGME49_277010 Toxoplasma gondii Probably essential sidik
TGME49_239320 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Rv3284 (Mycobacterium tuberculosis), Conserved hypothetical protein
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