Detailed view for Rv3580c

Basic information

TDR Targets ID: 981132
Mycobacterium tuberculosis, Cysteinyl-tRNA synthetase 1 CysS1 (cysteine--tRNA ligase 1) (CYSRS 1) (cysteine translase)
Source Database / ID:  Tuberculist 

pI: 6.3767 | Length (AA): 469 | MW (Da): 51822 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

Aminoacyl-tRNA biosynthesis (KEGG)

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 462 1u0b (B) 2 459 40.00 0 1 1.49368 -0.58
7 394 1li5 (B) 2 392 47.00 0 1 1.32869 -0.48

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126755)

Species Accession Gene Product
Arabidopsis thaliana AT5G38830   cysteinyl-tRNA synthetase
Arabidopsis thaliana AT3G56300   cysteinyl-tRNA synthetase
Arabidopsis thaliana AT2G31170   cysteinyl-tRNA synthetase
Babesia bovis BBOV_I002400   cysteinyl-tRNA synthetase, putative
Brugia malayi Bm1_29135   cysteinyl tRNA synthetase protein 1
Candida albicans CaO19.4931   cysteinyl-tRNA synthetase
Candida albicans CaO19.12397   cysteinyl-tRNA synthetase
Caenorhabditis elegans CELE_Y23H5A.7   Protein CARS-1, isoform B
Cryptosporidium hominis Chro.60125   cysteinyl-tRNA-synthetase
Cryptosporidium parvum cgd6_960   cysteinyl-tRNA synthetase
Chlamydia trachomatis CT_782   cysteine--tRNA ligase
Dictyostelium discoideum DDB_G0287159   cysteine-tRNA ligase
Dictyostelium discoideum DDB_G0277295   cysteine-tRNA ligase
Drosophila melanogaster Dmel_CG8431   Cysteinyl-tRNA synthetase
Drosophila melanogaster Dmel_CG8257   CG8257 gene product from transcript CG8257-RB
Escherichia coli b0526   cysteinyl-tRNA synthetase
Echinococcus granulosus EgrG_000793800   cysteinyl tRNA synthetase
Entamoeba histolytica EHI_169700   cysteinyl-tRNA synthetase, putative
Echinococcus multilocularis EmuJ_000003900   cysteinyl tRNA synthetase
Echinococcus multilocularis EmuJ_000793800   cysteinyl tRNA synthetase
Giardia lamblia GL50803_5867   Cysteinyl-tRNA synthetase
Homo sapiens ENSG00000134905   cysteinyl-tRNA synthetase 2, mitochondrial (putative)
Homo sapiens ENSG00000110619   cysteinyl-tRNA synthetase
Leishmania braziliensis LbrM.12.0270   cysteinyl-tRNA synthetase, putative
Leishmania donovani LdBPK_120255.1   cysteinyl-tRNA synthetase, putative
Leishmania infantum LinJ.12.0230   cysteinyl-tRNA synthetase, putative
Leishmania major LmjF.12.0250   cysteinyl-tRNA synthetase, putative
Leishmania mexicana LmxM.12.0250   cysteinyl-tRNA synthetase, putative
Loa Loa (eye worm) LOAG_02466   hypothetical protein
Loa Loa (eye worm) LOAG_09608   cysteinyl tRNA synthetase 1
Loa Loa (eye worm) LOAG_12019   hypothetical protein
Mycobacterium leprae ML0323   Probable cysteinyl-tRNA synthase 1 CysS1 (Cysteine--tRNA ligase 1) (CysRS 1)
Mus musculus ENSMUSG00000010755   cysteinyl-tRNA synthetase
Mus musculus ENSMUSG00000056228   cysteinyl-tRNA synthetase 2 (mitochondrial)(putative)
Mycobacterium tuberculosis Rv3580c   Cysteinyl-tRNA synthetase 1 CysS1 (cysteine--tRNA ligase 1) (CYSRS 1) (cysteine translase)
Mycobacterium ulcerans MUL_4156   cysteinyl-tRNA synthetase
Neospora caninum NCLIV_060470   hypothetical protein
Oryza sativa 4347821   Os09g0556500
Onchocerca volvulus OVOC2679  
Plasmodium berghei PBANKA_1213600   cysteine--tRNA ligase, putative
Plasmodium falciparum PF3D7_1015200   cysteine--tRNA ligase
Plasmodium knowlesi PKNH_0815300   cysteine--tRNA ligase, putative
Plasmodium vivax PVX_094980   cysteine--tRNA ligase, putative
Plasmodium yoelii PY04618   cysteinyl-tRNA synthetase
Saccharomyces cerevisiae YNL247W   cysteine--tRNA ligase
Schistosoma japonicum Sjp_0122730   ko:K01883 cysteinyl-tRNA synthetase [EC6.1.1.16A], putative
Schistosoma japonicum Sjp_0091610   Cysteinyl-tRNA synthetase, cytoplasmic, putative
Schistosoma mansoni Smp_170800.1   cysteinyl-tRNA synthetase
Schistosoma mansoni Smp_170800.2   cysteinyl-tRNA synthetase
Schmidtea mediterranea mk4.046967.00   Probable cysteineALQ-tRNA ligase, mitochondrial
Schmidtea mediterranea mk4.000333.10   CysteineALQ-tRNA ligase, cytoplasmic
Trypanosoma brucei gambiense Tbg972.6.720   cysteinyl-tRNA synthetase, putative
Trypanosoma brucei Tb927.6.950   cysteinyl-tRNA synthetase, putative
Trypanosoma congolense TcIL3000_0_28240   cysteinyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.507537.30   cysteinyl-tRNA synthetase, putative
Trypanosoma cruzi TcCLB.507297.20   cysteinyl-tRNA synthetase, putative
Toxoplasma gondii TGME49_299810   cysteine-tRNA synthetase (CysRS)
Treponema pallidum TP0091   cysteinyl-tRNA synthetase
Theileria parva TP03_0065   cysteinyl-tRNA synthetase, putative
Trichomonas vaginalis TVAG_276400   cysteinyl-tRNA synthetase, putative
Wolbachia endosymbiont of Brugia malayi Wbm0680   cysteinyl-tRNA synthetase

Essentiality

Rv3580c has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.950 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.950 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.950 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.6.950 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b0526 Escherichia coli essential goodall
CELE_Y23H5A.7 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y23H5A.7 Caenorhabditis elegans larval arrest wormbase
CELE_Y23H5A.7 Caenorhabditis elegans larval lethal wormbase
CELE_Y23H5A.7 Caenorhabditis elegans slow growth wormbase
CELE_Y23H5A.7 Caenorhabditis elegans sterile wormbase
YNL247W Saccharomyces cerevisiae inviable yeastgenome
TGME49_299810 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier Rv3580c (Mycobacterium tuberculosis), Cysteinyl-tRNA synthetase 1 CysS1 (cysteine--tRNA ligase 1) (CYSRS 1) (cysteine translase)
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