Detailed view for Rv3774

Basic information

TDR Targets ID: 5913
Mycobacterium tuberculosis, Possible enoyl-CoA hydratase EchA21 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase)
Source Database / ID:  Tuberculist 

pI: 4.753 | Length (AA): 274 | MW (Da): 29101 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00378   Enoyl-CoA hydratase/isomerase

Gene Ontology

Mouse over links to read term descriptions.
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 273 2ppy (A) 4 264 25.00 0 1 1.28085 -0.13
4 271 1dci (A) 53 321 40.00 0 1 1.4856 -0.43
8 245 2a7k (A) 2 227 27.00 0 1 1.23611 -0.89

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Dormant phase. hasan
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile Dormant phase. murphy
Show/Hide expression data references
  • hasan Prioritizing genomic drug targets in pathogens: application to Mycobacterium tuberculosis.
  • murphy Identification of gene targets against dormant phase Mycobacterium tuberculosis infections.

Orthologs

Ortholog group members (OG5_129459)

Species Accession Gene Product
Arabidopsis thaliana AT5G43280   delta(3,5),delta(2,4)-dienoyl-CoA isomerase 1
Brugia malayi Bm1_35505   enoyl-CoA hydratase/isomerase family protein
Candida albicans CaO19.14122   enoyl-CoA hydratase/isomerase family protein similar to N. crassa sequence (CAD21495)
Candida albicans CaO19.6830   enoyl-CoA hydratase/isomerase family protein similar to N. crassa sequence (CAD21495)
Caenorhabditis elegans CELE_Y25C1A.13   Protein Y25C1A.13
Caenorhabditis elegans CELE_F58A6.1   Protein F58A6.1
Dictyostelium discoideum DDB_G0282261   Delta-Delta-dienoyl-CoA isomerase
Drosophila melanogaster Dmel_CG9577   CG9577 gene product from transcript CG9577-RA
Homo sapiens ENSG00000104823   enoyl CoA hydratase 1, peroxisomal
Leishmania braziliensis LbrM.18.0640   peroxisomal enoyl-coa hydratase, putative
Leishmania donovani LdBPK_180580.1   peroxisomal enoyl-coa hydratase, putative
Leishmania infantum LinJ.18.0580   peroxisomal enoyl-coa hydratase, putative
Leishmania major LmjF.18.0580   peroxisomal enoyl-coa hydratase, putative
Leishmania mexicana LmxM.18.0580   peroxisomal enoyl-coa hydratase, putative
Loa Loa (eye worm) LOAG_07056   enoyl-CoA hydratase/isomerase
Mycobacterium leprae ML0120c   Possible enoyl-CoA hydratase EchA21 (enoyl hydrase) (unsaturated acyl-coA hydratase) (crotonase)
Mus musculus ENSMUSG00000053898   enoyl coenzyme A hydratase 1, peroxisomal
Mycobacterium tuberculosis Rv3774   Possible enoyl-CoA hydratase EchA21 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase)
Neospora caninum NCLIV_054880   Short chain enoyl-CoA hydratase (EC 4.2.1.17), related
Oryza sativa 4327232   Os01g0925200
Onchocerca volvulus OVOC11591  
Schmidtea mediterranea mk4.013378.00   Delta(3,5)-Delta(2,4)-dienoyl-CoA isomerase, mitochondrial
Trypanosoma cruzi TcCLB.511277.210   peroxisomal enoyl-coa hydratase, putative
Toxoplasma gondii TGME49_310830   enoyl-CoA hydratase/isomerase family protein

Essentiality

Rv3774 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
mtu3841 this record Mycobacterium tuberculosis non-essential nmpdr
CELE_F58A6.1 Caenorhabditis elegans slow growth wormbase
CELE_Y25C1A.13 Caenorhabditis elegans larval arrest wormbase
TGME49_310830 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Crotonase (4.2.1.17 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Mycobacterium smegmatis ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Rv3774 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Mycobacterium smegmatis ( 1 )

Bibliographic References

4 literature references were collected for this gene.

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Gene identifier Rv3774 (Mycobacterium tuberculosis), Possible enoyl-CoA hydratase EchA21 (enoyl hydrase) (unsaturated acyl-CoA hydratase) (crotonase)
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