Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | ectonucleotide pyrophosphatase/phosphodiesterase 2 | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Loa Loa (eye worm) | hypothetical protein | 0.004 | 0.1195 | 0.1195 |
Echinococcus multilocularis | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Echinococcus multilocularis | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Echinococcus granulosus | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Echinococcus granulosus | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Echinococcus multilocularis | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Onchocerca volvulus | 0.0087 | 1 | 1 | |
Echinococcus granulosus | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Echinococcus multilocularis | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Schistosoma mansoni | hypothetical protein | 0.0087 | 1 | 1 |
Echinococcus granulosus | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Loa Loa (eye worm) | thrombospondin type 1 domain-containing protein | 0.0087 | 1 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.004 | 0.1195 | 0.1195 |
Echinococcus multilocularis | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Echinococcus multilocularis | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.004 | 0.1195 | 0.1195 |
Echinococcus granulosus | ectonucleotide pyrophosphatase:phosphodiesterase | 0.004 | 0.1195 | 1 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 25 nM | BindingDB_Patents: Inhibition Assay. 10 µL of a test compound solution (10% dimethyl sulfoxide) at each concentration and 40 µL of a 5 µg/mL human ENPP2 solution (buffer A: 100 mmol/L Tris-HCl (pH 9.0), 500 mmol/L NaCl, 5 mmol/L MgCl2, 0.05% Triton X-100) were mixed, 50 µL of a 2 mmol/L 16:0-lysophosphatidylcholine (LPC) solution (buffer A) was further added to react at 37° C. for 24 hours. Subsequently, to 10 µL of the reaction solution was added 90 µL of a measurement buffer (0.5 mmol/L aminoantipyrine, 0.3 mmol/L N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3-methylaniline, 1 U/mL peroxidase, 3 U/mL choline oxidase, 100 mmol/L Tris-HCl (pH 8.5), 5 mmol/L CaCl2) to react at 37° C. for 20 minutes, and spectrophotometric determination was performed at 555 nm. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.