Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Mycobacterium leprae | Probable adenosine deaminase Add (ADENOSINE AMINOHYDROLASE) | 0.0065 | 1 | 0.5 |
Schistosoma mansoni | adenosine deaminase-related | 0.0065 | 1 | 1 |
Trichomonas vaginalis | adenosine deaminase, putative | 0.0065 | 1 | 0.5 |
Entamoeba histolytica | adenosine deaminase, putative | 0.0065 | 1 | 0.5 |
Mycobacterium ulcerans | adenosine deaminase | 0.0065 | 1 | 0.5 |
Toxoplasma gondii | Adenosine/AMP deaminase domain-containing protein | 0.0065 | 1 | 0.5 |
Echinococcus granulosus | adenosine deaminase | 0.0065 | 1 | 1 |
Onchocerca volvulus | Adenosine deaminase homolog | 0.0065 | 1 | 1 |
Mycobacterium tuberculosis | Probable adenosine deaminase Add (adenosine aminohydrolase) | 0.0065 | 1 | 0.5 |
Schistosoma mansoni | adenosine deaminase | 0.0065 | 1 | 1 |
Trichomonas vaginalis | adenosine deaminase, putative | 0.0065 | 1 | 0.5 |
Echinococcus multilocularis | adenosine deaminase | 0.0065 | 1 | 1 |
Plasmodium falciparum | adenosine deaminase | 0.0065 | 1 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0065 | 1 | 1 |
Entamoeba histolytica | adenosine deaminase, putative | 0.0065 | 1 | 0.5 |
Leishmania major | adenine aminohydrolase | 0.0065 | 1 | 0.5 |
Plasmodium vivax | adenosine deaminase, putative | 0.0065 | 1 | 0.5 |
Toxoplasma gondii | Adenosine/AMP deaminase domain-containing protein | 0.0065 | 1 | 0.5 |
Treponema pallidum | adenosine deaminase | 0.0065 | 1 | 0.5 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 55280 nM | BindingDB_Patents: Alphascreen Assay. The AlphaScreen assay was performed according to the manufacturer's protocol (Perkin Elmer, Benelux). Reactions were performed in 25 ul final volume in 384-well Optiwell microtiter plates (Perkin Elmer). The reaction buffer contained 25 mM Tris-HCl (pH 7.4), 150 mM NaCl, 1 mM MgCl2, 0.01% (v/v) Tween-20 and 0.1% (w/v) bovine serum albumin. His6-tagged integrase (300 nM final concentration) was incubated with the compounds for 30 min at 4C. The compounds were added at varying concentrations spanning a wide range from 0.1 up to 100 uM. Afterwards 100 nM flag-LEDGF/p75 was added and incubation was prolonged for an additional hour at 4C. Subsequently 5 ul of Ni-chelate-coated acceptor beads and 5 ul anti-flag donor beads were added to a final concentration of 20 ug/ml of both beads. Proteins and beads were incubated for 1 h at 30C. in order to allow association to occur. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.