Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | solute carrier family 8 (sodium/calcium exchanger), member 1 | Starlite/ChEMBL | No references |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Trypanosoma cruzi | choline kinase | 0.0023 | 0 | 0.5 |
Giardia lamblia | Ethanolamine kinase, putative | 0.0023 | 0 | 0.5 |
Schistosoma mansoni | sodium/calcium exchanger | 0.0091 | 0.3186 | 1 |
Trypanosoma brucei | choline kinase | 0.0023 | 0 | 0.5 |
Echinococcus multilocularis | sodium calcium exchanger | 0.0091 | 0.3186 | 0.3186 |
Trypanosoma brucei | choline/ethanolamine kinase | 0.0023 | 0 | 0.5 |
Echinococcus granulosus | choline:ethanolamine kinase | 0.0237 | 1 | 1 |
Toxoplasma gondii | phosphotransferase enzyme family protein | 0.0237 | 1 | 1 |
Loa Loa (eye worm) | solute carrier family 8 | 0.0091 | 0.3186 | 0.3186 |
Entamoeba histolytica | choline/ethanolamine kinase, putative | 0.0023 | 0 | 0.5 |
Echinococcus multilocularis | choline:ethanolamine kinase | 0.0237 | 1 | 1 |
Leishmania major | choline kinase | 0.0023 | 0 | 0.5 |
Leishmania major | choline/ethanolamine kinase, putative | 0.0023 | 0 | 0.5 |
Loa Loa (eye worm) | choline/ethanolamine kinase | 0.0237 | 1 | 1 |
Onchocerca volvulus | 0.0023 | 0 | 0.5 | |
Brugia malayi | Sodium/calcium exchanger protein | 0.0036 | 0.0631 | 0.0631 |
Plasmodium falciparum | choline kinase | 0.0237 | 1 | 1 |
Entamoeba histolytica | choline/ethanolamine kinase, putative | 0.0023 | 0 | 0.5 |
Trypanosoma cruzi | choline ethanolamine kinase, putative | 0.0023 | 0 | 0.5 |
Onchocerca volvulus | Putative choline\/ethanolamine kinase | 0.0023 | 0 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0036 | 0.0631 | 0.0631 |
Echinococcus granulosus | sodium calcium exchanger | 0.0091 | 0.3186 | 0.3186 |
Trichomonas vaginalis | choline/ethanolamine kinase, putative | 0.0023 | 0 | 0.5 |
Plasmodium vivax | choline kinase, putative | 0.0237 | 1 | 1 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 3300 nM | BindingDB_Patents: Forward Mode Assay. The assay is based on the monitoring of intracellular Ca2+concentrations using the PBX Calcium Assay Kit from BD (Becton, Dickinson and Company) with calcium indicator dye 51-9000177BKa (BD, 640177), CHO cells expressing NCX1 were loaded with the dye, and after a preincubation period with the test compound, lonomycin (Calbiochem, 407950) was added. Ionomycin is an ionophor for Ca2+ions mediating an increase of intracellular Ca2+ions. Consequently, intracellular Ca2+ions are exchanged against extracellular Na+ions (Ca2+efflux, forward mode). The decrease of intracellular Ca2+ions was detected by measuring the fluorescence of the calcium indicator dye at a wavelength of 520 nm by a FLIPR device.Briefly, similarly as for the reverse mode, for the forward mode transport assay 18000 cells per well were seeded into a 96 well microplate (Corning COSTAR 3904) and incubated overnight in culture medium (cf. above). A total volume of 100 ul medium per well was used. | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.