Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | potassium voltage-gated channel, subfamily H (eag-related), member 2 | Starlite/ChEMBL | References |
Homo sapiens | potassium inwardly-rectifying channel, subfamily J, member 1 | Starlite/ChEMBL | References |
Rattus norvegicus | ATP-sensitive inward rectifier potassium channel 1 | Starlite/ChEMBL | References |
Species | Potential target | Known druggable target | Length | Alignment span | Identity |
---|---|---|---|---|---|
Onchocerca volvulus | ATP-sensitive inward rectifier potassium channel 1 | 391 aa | 333 aa | 38.4 % | |
Onchocerca volvulus | ATP-sensitive inward rectifier potassium channel 1 | 391 aa | 332 aa | 44.6 % | |
Loa Loa (eye worm) | inward rectifying k channel family protein 1 | ATP-sensitive inward rectifier potassium channel 1 | 391 aa | 329 aa | 38.9 % |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Echinococcus granulosus | potassium voltage gated channel subfamily H | 0.0013 | 0.021 | 0.1145 |
Schistosoma mansoni | voltage-gated potassium channel | 0.0013 | 0.021 | 0.1028 |
Loa Loa (eye worm) | hypothetical protein | 0.0039 | 0.1529 | 0.1529 |
Brugia malayi | Voltage-gated potassium channel, HERG (KCNH2)-related. C. elegans unc-103 ortholog | 0.0045 | 0.183 | 1 |
Echinococcus multilocularis | potassium voltage gated channel subfamily H | 0.0045 | 0.183 | 1 |
Echinococcus multilocularis | potassium voltage gated channel subfamily H | 0.0013 | 0.021 | 0.1145 |
Loa Loa (eye worm) | inward rectifying k channel family protein 1 | 0.0204 | 1 | 1 |
Schistosoma mansoni | voltage-gated potassium channel | 0.0049 | 0.2039 | 1 |
Echinococcus granulosus | potassium voltage gated channel subfamily H | 0.0045 | 0.183 | 1 |
Loa Loa (eye worm) | hypothetical protein | 0.0204 | 1 | 1 |
Echinococcus granulosus | voltage gated potassium channel | 0.0013 | 0.021 | 0.1145 |
Brugia malayi | Voltage-gated potassium channel, EAG (KCNH1)-related. C. elegans egl-2 ortholog | 0.0013 | 0.021 | 0.1145 |
Loa Loa (eye worm) | hypothetical protein | 0.0204 | 1 | 1 |
Trichomonas vaginalis | voltage and ligand gated potassium channel, putative | 0.0042 | 0.168 | 1 |
Toxoplasma gondii | hypothetical protein | 0.0204 | 1 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0013 | 0.021 | 0.021 |
Echinococcus multilocularis | voltage gated potassium channel | 0.0013 | 0.021 | 0.1145 |
Trichomonas vaginalis | voltage and ligand gated potassium channel, putative | 0.0042 | 0.168 | 1 |
Loa Loa (eye worm) | voltage and ligand gated potassium channel | 0.0045 | 0.183 | 0.183 |
Schistosoma mansoni | voltage-gated potassium channel | 0.0013 | 0.021 | 0.1028 |
Schistosoma mansoni | voltage-gated potassium channel | 0.0049 | 0.2039 | 1 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 20 nM | BindingDB_Patents: Electrophysiology Assay. Block of Kir1.1 (ROMKI) currents was examined by whole cell voltage clamp (Hamill et. al. Pfluegers Archives 391:85-100 (1981)) using the IonWorks Quattro automated electrophysiology platform (Molecular Devices, Sunnyvale, Calif.). Chinese hamster ovary cells stably expressing Kir1.1 channels were maintained in T-75 flasks in cell culture media in a humidified 10% CO2 incubator at 37° C. Prior to an experiment, Kir1.1 expression was induced by overnight incubation with 1 mM sodium butyrate. On the day of the experiment, cells were dissociated with 2.5 ml of Versene (Invitrogen 15040-066) for approximately 6 min at 37° C. and suspended in 10 ml of bath solution containing (in mM): 150 NaCl, 10 KCl, 2.7 CaCl2, 0.5 MgCl2, 5 HEPES, pH 7.4. After centrifugation, the cell pellet was resuspended in approximately 4.0 ml of bath solution and placed in the IonWorks instrument. The intracellular solution consisted of (in mM): 80 K gluconate, 40 KCl, 20 KF, 3.2 MgCl2, 3 EGTA, 5 Hepes, pH 7.4. | ChEMBL. | No reference |
IC50 (binding) | = 20 nM | BindingDB_Patents: Electrophysiology Assay. Block of Kir1.1 (ROMKI) currents was examined by whole cell voltage clamp (Hamill et. al. Pfluegers Archives 391:85-100 (1981)) using the IonWorks Quattro automated electrophysiology platform (Molecular Devices, Sunnyvale, Calif.). Chinese hamster ovary cells stably expressing Kir1.1 channels were maintained in T-75 flasks in cell culture media in a humidified 10% CO2 incubator at 37° C. Prior to an experiment, Kir1.1 expression was induced by overnight incubation with 1 mM sodium butyrate. On the day of the experiment, cells were dissociated with 2.5 ml of Versene (Invitrogen 15040-066) for approximately 6 min at 37° C. and suspended in 10 ml of bath solution containing (in mM): 150 NaCl, 10 KCl, 2.7 CaCl2, 0.5 MgCl2, 5 HEPES, pH 7.4. After centrifugation, the cell pellet was resuspended in approximately 4.0 ml of bath solution and placed in the IonWorks instrument. The intracellular solution consisted of (in mM): 80 K gluconate, 40 KCl, 20 KF, 3.2 MgCl2, 3 EGTA, 5 Hepes, pH 7.4. | ChEMBL. | No reference |
IC50 (binding) | = 0.02 uM | Inhibition of human ROMK expressed in CHO cells by whole-cell voltage clamp method | ChEMBL. | 27017115 |
IC50 (binding) | = 0.056 uM | Inhibition of rat ROMK expressed in HEK293 cells after 30 mins by [86Rb+] flux functional assay | ChEMBL. | 27017115 |
IC50 (binding) | = 0.06 uM | Inhibition of rat ROMK assessed as thallium flux after 30 mins in presence of ouabain by cell based FLIPR assay | ChEMBL. | 27017115 |
IC50 (binding) | = 17 uM | Inhibition of human ERG by electrophysiology analysis | ChEMBL. | 27017115 |
IC50 (binding) | = 29 uM | Displacement of [35S]-MK499 from human ERG expressed in HEK293 cells | ChEMBL. | 27017115 |
IC50 (binding) | > 30 uM | Inhibition of Nav1.2 (unknown origin) | ChEMBL. | 27017115 |
IC50 (binding) | > 30 uM | Inhibition of Cav2.1 (unknown origin) | ChEMBL. | 27017115 |
IC50 (binding) | > 50 uM | Inhibition of Kir2.3 (unknown origin) | ChEMBL. | 27017115 |
IC50 (binding) | > 50 uM | Inhibition of Kir2.1 (unknown origin) | ChEMBL. | 27017115 |
IC50 (ADMET) | > 50 uM | Inhibition of CYP3A4 (unknown origin) | ChEMBL. | 27017115 |
IC50 (ADMET) | > 50 uM | Inhibition of CYP2D6 (unknown origin) | ChEMBL. | 27017115 |
IC50 (ADMET) | > 50 uM | Inhibition of CYP2C9 (unknown origin) | ChEMBL. | 27017115 |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.
1 literature reference was collected for this gene.