Species | Target name | Source | Bibliographic reference |
---|---|---|---|
Homo sapiens | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 | No references | |
Homo sapiens | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4 | No references |
Species | Potential target | Known druggable target | Length | Alignment span | Identity |
---|---|---|---|---|---|
Trypanosoma brucei | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 | 500 aa | 429 aa | 24.9 % |
Species | Potential target | Raw | Global | Species |
---|---|---|---|---|
Trypanosoma brucei | 6-phosphofructo-2-kinase 2 | 0.0139 | 0.9706 | 0.9706 |
Mycobacterium ulcerans | hypothetical protein | 0.0083 | 0.2852 | 0.5 |
Giardia lamblia | Hypothetical protein | 0.0083 | 0.2852 | 0.5 |
Trypanosoma cruzi | 6-phosphofructo-2-kinase 1 | 0.0139 | 0.9706 | 0.9706 |
Entamoeba histolytica | phosphoglycerate mutase family protein, putative | 0.0083 | 0.2852 | 0.5 |
Schistosoma mansoni | 6-phosphofructokinase | 0.0141 | 1 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0141 | 1 | 1 |
Trypanosoma brucei | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative | 0.0141 | 1 | 1 |
Leishmania major | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative | 0.0139 | 0.9706 | 0.9706 |
Echinococcus multilocularis | 6 phosphofructo 2 kinase:fructose 2 | 0.0141 | 1 | 0.5 |
Loa Loa (eye worm) | hypothetical protein | 0.0139 | 0.9706 | 0.9604 |
Trypanosoma cruzi | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative | 0.0141 | 1 | 1 |
Giardia lamblia | Hypothetical protein | 0.0083 | 0.2852 | 0.5 |
Onchocerca volvulus | 0.0141 | 1 | 0.5 | |
Leishmania major | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative | 0.0141 | 1 | 1 |
Trypanosoma cruzi | 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase, putative | 0.0141 | 1 | 1 |
Mycobacterium ulcerans | fructose-2,6-bisphosphatase GpmB | 0.0083 | 0.2852 | 0.5 |
Trypanosoma cruzi | 6-phosphofructo-2-kinase 1 | 0.0139 | 0.9706 | 0.9706 |
Activity type | Activity value | Assay description | Source | Reference |
---|---|---|---|---|
IC50 (binding) | = 30 nM | Kinase Assay (Method A) | BINDINGDB. | No reference |
IC50 (binding) | = 60 nM | Kinase Assay (Method B) | BINDINGDB. | No reference |
IC50 (binding) | = 0.03 uM | Inhibition of PFKFB3 (unknown origin) expressed in Escherichia coli assessed as fructose -2,6-bisphosphate level using fructose-6-phosphate as substrate and ATP measured after 10 mins by spectrophotometry | ChEMBL. | No reference |
IC50 (binding) | = 0.06 uM | Inhibition of PFKFB4 (unknown origin) expressed in Escherichia coli assessed as ADP level preincubated for 15 mins followed by addition of fructose-6-phosphate as substrate and ATP measured after 40 mins by luciferase assay | ChEMBL. | No reference |
IC50 (binding) | = 2.3 uM | Inhibition of PFKFB3/PFKFB4 in human Panc1 cells assessed as fructose -2,6-bisphosphate production preincubated for 1 hr followed by addition of D-glucose measured after 2 hrs by spectrophotometry | ChEMBL. | No reference |
Many chemical entities in TDR Targets come from high-throughput screenings with whole cells or tissue samples, and not all assayed compounds have been tested against a single a single target protein, probably because they get ruled out during screening process. Even if these compounds may have not been of interest in the original screening, they may come as interesting leads for other screening assays. Furthermore, we may be able to propose drug-target associations using chemical similarities and network patterns.