Detailed view for Tb927.6.400

Basic information

TDR Targets ID: 10754
Trypanosoma brucei, peptidase M20/M25/M40, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.058 | Length (AA): 475 | MW (Da): 52185 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01546   Peptidase family M20/M25/M40

Gene Ontology

Mouse over links to read term descriptions.
GO:0016787   hydrolase activity  
GO:0008237   metallopeptidase activity  
GO:0008152   metabolic process  
GO:0006508   proteolysis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Bloodstream Form. Siegel TN
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127342)

Species Accession Gene Product
Brugia malayi Bm1_37280   hypothetical protein
Candida albicans CaO19.11397   similar to S. cerevisiae YFR044C
Candida albicans CaO19.3915   similar to S. cerevisiae YFR044C
Caenorhabditis elegans CELE_Y71H2AM.11   Protein Y71H2AM.11
Caenorhabditis elegans CELE_R11H6.1   Protein PES-9
Dictyostelium discoideum DDB_G0279291   hypothetical protein
Drosophila melanogaster Dmel_CG17337   CG17337 gene product from transcript CG17337-RA
Echinococcus granulosus EgrG_000992300   cytosolic non specific dipeptidase
Echinococcus multilocularis EmuJ_000992300   cytosolic non specific dipeptidase
Homo sapiens ENSG00000133313   CNDP dipeptidase 2 (metallopeptidase M20 family)
Homo sapiens ENSG00000150656   carnosine dipeptidase 1 (metallopeptidase M20 family)
Leishmania braziliensis LbrM.33.1880   peptidase M20/M25/M40, putative
Leishmania braziliensis LbrM.31.2100   peptidase m20/m25/m40 family-like protein
Leishmania braziliensis LbrM.31.2120   peptidase m20/m25/m40 family-like protein
Leishmania braziliensis LbrM.33.2100   peptidase M20/M25/M40, putative
Leishmania braziliensis LbrM.31.2280   succinyl-diaminopimelate desuccinylase-like protein
Leishmania donovani LdBPK_331710.1   cytosolic nonspecific dipeptidase, putative
Leishmania donovani LdBPK_312060.1   succinyl-diaminopimelate desuccinylase-like protein
Leishmania infantum LinJ.33.1710   peptidase M20/M25/M40, putative
Leishmania infantum LinJ.31.2060   succinyl-diaminopimelate desuccinylase-like protein
Leishmania major LmjF.33.1610   peptidase M20/M25/M40, putative
Leishmania major LmjF.31.1890   peptidase m20/m25/m40 family-like protein
Leishmania major LmjF.31.2020   succinyl-diaminopimelate desuccinylase-like protein
Leishmania mexicana LmxM.30.2020   succinyl-diaminopimelate desuccinylase-like protein
Leishmania mexicana LmxM.30.1890   peptidase m20/m25/m40 family-like protein
Leishmania mexicana LmxM.32.1610   peptidase M20/M25/M40, putative
Loa Loa (eye worm) LOAG_10052   cytosolic non-specific dipeptidase
Mycobacterium leprae ML1193   conserved hypothetical protein
Mus musculus ENSMUSG00000024644   CNDP dipeptidase 2 (metallopeptidase M20 family)
Mus musculus ENSMUSG00000056162   carnosine dipeptidase 1 (metallopeptidase M20 family)
Mycobacterium tuberculosis Rv2522c   Conserved hypothetical protein
Mycobacterium ulcerans MUL_3812   hypothetical protein
Saccharomyces cerevisiae YFR044C   metallodipeptidase
Schmidtea mediterranea mk4.025490.00  
Schmidtea mediterranea mk4.033352.00  
Schmidtea mediterranea mk4.003046.02  
Schmidtea mediterranea mk4.010005.00  
Trypanosoma brucei gambiense Tbg972.10.14780   cytosolic nonspecific dipeptidase, putative,peptidase (M20/M25/M40 family), putative
Trypanosoma brucei gambiense Tbg972.6.80   peptidase M20/M25/M40, putative
Trypanosoma brucei Tb927.6.400   peptidase M20/M25/M40, putative
Trypanosoma brucei Tb927.10.12260   cytosolic nonspecific dipeptidase, putative
Trypanosoma congolense TcIL3000_10_10490   cytosolic nonspecific dipeptidase, putative
Trypanosoma cruzi TcCLB.510257.80   cytosolic nonspecific dipeptidase, putative
Trypanosoma cruzi TcCLB.509213.120   cytosolic nonspecific dipeptidase, putative
Toxoplasma gondii TGME49_213060   WD domain, G-beta repeat-containing protein
Trichomonas vaginalis TVAG_370490   Clan MH, family M20, peptidase T-like metallopeptidase
Trichomonas vaginalis TVAG_224980   Clan MH, family M20, peptidase T-like metallopeptidase

Essentiality

Tb927.6.400 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.6.400 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.6.400 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.6.400 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.6.400 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.12260 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.12260 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.12260 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.12260 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_213060 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.6.400 (Trypanosoma brucei), peptidase M20/M25/M40, putative
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