Detailed view for Tb927.10.14470

Basic information

TDR Targets ID: 13250
Trypanosoma brucei, intraflagellar transport protein 140
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.51 | Length (AA): 1696 | MW (Da): 186611 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

No Pfam domain information for this protein.

Gene Ontology

Mouse over links to read term descriptions.
GO:0005515   protein binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
35 154 4lg9 (A) 178 298 29.00 0.071 0.49 0.187055 -0.44
63 149 4lg8 (A) 342 419 35.00 0.0048 1 0.255597 -0.21
68 161 5naf (C) 171 264 29.00 0.053 1 0.397725 -0.39
860 1045 3mkq (A) 619 806 17.00 0 0.9 0.30827 -0.06
893 1034 3mkq (B) 648 787 14.00 0 1 0.310326 -0.35
1229 1492 5cqs (C) 956 1341 14.00 0 1 0.26126 0.38
1236 1375 2fo7 (A) 3 131 27.00 0.93 0.88 0.187847 0.26
1475 1589 5hrz (A) 4 116 11.00 0.0017 0.08 0.175107 -0.29
1501 1695 4zlh (A) 93 294 10.00 0.55 0.07 0.161276 -0.09

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130315)

Species Accession Gene Product
Caenorhabditis elegans CELE_C27A7.4   Protein CHE-11
Drosophila melanogaster Dmel_CG11838   reduced mechanoreceptor potential A
Echinococcus granulosus EgrG_000374900   intraflagellar transport protein 140
Echinococcus multilocularis EmuJ_000374900   intraflagellar transport protein 140
Homo sapiens ENSG00000187535   intraflagellar transport 140
Leishmania braziliensis LbrM.32.0380   hypothetical protein, conserved
Leishmania donovani LdBPK_320320.1   intraflagellar transport protein 140, putative
Leishmania infantum LinJ.32.0320   hypothetical protein, conserved
Leishmania major LmjF.32.0310   hypothetical protein, conserved
Leishmania mexicana LmxM.31.0310   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_15585   hypothetical protein
Loa Loa (eye worm) LOAG_06991   hypothetical protein
Loa Loa (eye worm) LOAG_12707   hypothetical protein
Mus musculus ENSMUSG00000024169   intraflagellar transport 140
Neospora caninum NCLIV_052170   hypothetical protein, conserved
Onchocerca volvulus OVOC10121  
Onchocerca volvulus OVOC10126  
Schistosoma japonicum Sjp_0108670   IPR008262,Lipase, active site;IPR001680,WD-40 repeat;IPR011046,WD40-like,domain-containing
Schistosoma japonicum Sjp_0101550   Conserved hypothetical protein
Schistosoma mansoni Smp_163250   hypothetical protein
Schmidtea mediterranea mk4.027327.00  
Schmidtea mediterranea mk4.013795.00  
Schmidtea mediterranea mk4.004640.01   Intraflagellar transport protein 140 homolog
Schmidtea mediterranea mk4.004640.00   Intraflagellar transport protein 140 homolog
Trypanosoma brucei gambiense Tbg972.10.17590   hypothetical protein, conserved
Trypanosoma brucei Tb927.10.14470   intraflagellar transport protein 140
Trypanosoma congolense TcIL3000_10_12370   intraflagellar transport protein 140, putative
Trypanosoma cruzi TcCLB.506215.19   intraflagellar transport protein 140, putative
Toxoplasma gondii TGME49_215195   tetratricopeptide repeat-containing protein
Toxoplasma gondii TGME49_215185   hypothetical protein
Trichomonas vaginalis TVAG_083840   conserved hypothetical protein
Trichomonas vaginalis TVAG_404040   conserved hypothetical protein
Trichomonas vaginalis TVAG_003730   conserved hypothetical protein
Trichomonas vaginalis TVAG_483890   conserved hypothetical protein
Trichomonas vaginalis TVAG_310210   conserved hypothetical protein

Essentiality

Tb927.10.14470 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.14470 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.14470 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.14470 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.14470 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_215195 Toxoplasma gondii Probably non-essential sidik
TGME49_215185 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.10.14470 (Trypanosoma brucei), intraflagellar transport protein 140
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