Detailed view for Tb927.8.3310

Basic information

TDR Targets ID: 14274
Trypanosoma brucei, Elongator-like Protein 3b
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.8923 | Length (AA): 666 | MW (Da): 76043 | Paralog Number: 2

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00583   Acetyltransferase (GNAT) family
PF04055   Radical SAM superfamily
PF16199   Radical_SAM C-terminal domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0051536   iron-sulfur cluster binding  
GO:0008080   N-acetyltransferase activity  
GO:0003824   catalytic activity  
GO:0008152   metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127324)

Species Accession Gene Product
Arabidopsis thaliana AT5G50320   Elongator complex protein 3
Babesia bovis BBOV_III010020   histone acetyltransferase, ELP3 family protein
Brugia malayi Bm1_32255   RE35395p
Candida albicans CaO19.7387   Elongator Protein
Caenorhabditis elegans CELE_ZK863.3   Protein ELPC-3
Cryptosporidium hominis Chro.10275   hypothetical protein
Cryptosporidium parvum cgd1_2450   ELP3 like acetyltransferase involved in transcription
Dictyostelium discoideum DDB_G0290103   GCN5-related N-acetyltransferase
Drosophila melanogaster Dmel_CG15433   Elongator complex protein 3
Echinococcus granulosus EgrG_000482000   elongator complex protein 3
Entamoeba histolytica EHI_152010   histone acetyltransferase, putative
Echinococcus multilocularis EmuJ_000482000   elongator complex protein 3
Giardia lamblia GL50803_16639   Histone acetyltransferase Elp3
Homo sapiens ENSG00000134014   elongator acetyltransferase complex subunit 3
Leishmania braziliensis LbrM.23.1470   acetyltransferase-like protein
Leishmania braziliensis LbrM.16.0250   histone acetyltransferase-like protein
Leishmania donovani LdBPK_160250.1   histone acetyltransferase-like protein
Leishmania donovani LdBPK_231610.1   acetyltransferase-like protein
Leishmania infantum LinJ.16.0250   histone acetyltransferase-like protein
Leishmania infantum LinJ.23.1610   acetyltransferase-like protein
Leishmania major LmjF.23.1350   acetyltransferase-like protein
Leishmania major LmjF.16.0240   histone acetyltransferase-like protein
Leishmania mexicana LmxM.23.1350   acetyltransferase-like protein
Leishmania mexicana LmxM.16.0240   histone acethyltransferase-like protein
Loa Loa (eye worm) LOAG_09530   hypothetical protein
Loa Loa (eye worm) LOAG_11221   hypothetical protein
Mus musculus ENSMUSG00000022031   elongator acetyltransferase complex subunit 3
Neospora caninum NCLIV_043970   elongator complex protein 3, putative
Oryza sativa 4336205   Os04g0484900
Plasmodium berghei PBANKA_1442500   elongator complex protein 3, putative
Plasmodium falciparum PF3D7_1227800   elongator complex protein 3, putative
Plasmodium knowlesi PKNH_1447200   elongator complex protein 3, putative
Plasmodium yoelii PY03999   hypothetical protein
Saccharomyces cerevisiae YPL086C   Elongator subunit ELP3
Schistosoma japonicum Sjp_0067110   Probable elongator complex protein 3, putative
Schistosoma japonicum Sjp_0096190   Elongator complex protein 3, putative
Schistosoma japonicum Sjp_0067100   ko:K07739 histone acetyltransferase, ELP3 family, putative
Schistosoma mansoni Smp_085500   histone acetyltransferase-related
Schmidtea mediterranea mk4.003323.03   Probable elongator complex protein 3
Trypanosoma brucei gambiense Tbg972.8.5770   histone acethyltransferase, putative
Trypanosoma brucei gambiense Tbg972.8.3070   acetyltransferase, putative
Trypanosoma brucei Tb11.v5.0520   acetyltransferase, putative
Trypanosoma brucei Tb927.8.3310   Elongator-like Protein 3b
Trypanosoma brucei Tb927.8.5770   Elongator-like Protein 3a
Trypanosoma congolense TcIL3000_8_5560   histone acetyltransferase, putative
Trypanosoma congolense TcIL3000_8_3370   acetyltransferase, putative
Trypanosoma cruzi TcCLB.506743.120   Elongator-like Protein 3a, putative
Trypanosoma cruzi TcCLB.503851.10   Elongator-like Protein 3a, putative
Trypanosoma cruzi TcCLB.509769.110   Elongator-like Protein 3b, putative
Toxoplasma gondii TGME49_305480   elongator complex protein ELP3
Theileria parva TP02_0146   hypothetical protein, conserved
Trichomonas vaginalis TVAG_028740   elongator complex protein, putative

Essentiality

Tb927.8.3310 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.5770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.5770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.5770 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.5770 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.8.3310 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.8.3310 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.3310 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.3310 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1442500 Plasmodium berghei Essential plasmo
TGME49_305480 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0003 0.5 0.5
0.0004 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.8.3310 (Trypanosoma brucei), Elongator-like Protein 3b
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