Detailed view for Tb927.11.16970

Basic information

TDR Targets ID: 15381
Trypanosoma brucei, protein phosphatase 4, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.7424 | Length (AA): 303 | MW (Da): 34432 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00149   Calcineurin-like phosphoesterase

Gene Ontology

Mouse over links to read term descriptions.
GO:0000159   protein phosphatase type 2A complex  
GO:0042578   phosphoric ester hydrolase activity  
GO:0016787   hydrolase activity  
GO:0004722   protein serine/threonine phosphatase activity  
GO:0006796   phosphate metabolic process  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 303 3fga (C) 7 309 57.00 0 1 1.7708 -1.38
1 147 4iyp (C) 7 153 58.00 0 1 0.896949 -0.02
1 297 5jpe (A) 170 475 39.00 0 1 1.57 -1.44

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127639)

Species Accession Gene Product
Arabidopsis thaliana AT5G55260   serine/threonine-protein phosphatase PP-X isozyme 2
Arabidopsis thaliana AT4G26720   serine/threonine-protein phosphatase PP-X isozyme 1
Babesia bovis BBOV_III010990   Ser/Thr protein phosphatase family protein
Brugia malayi Bm1_28355   serine/threonine protein phosphatase 4 catalytic subunit
Candida albicans CaO19.11856   Protein serine/threonine phosphatase, PPP family
Candida albicans CaO19.4350   Protein serine/threonine phosphatase 2A
Candida albicans CaO19.4378   Protein serine/threonine phosphatase, PPP family
Candida albicans CaO19.11828   Protein serine/threonine phosphatase 2A
Caenorhabditis elegans CELE_Y49E10.3   Protein PPH-4.2, isoform B
Caenorhabditis elegans CELE_Y75B8A.30   Protein PPH-4.1
Cryptosporidium hominis Chro.10265   protein phosphatase 4 (formerly X), catalytic subunit; Protein phosphatase 4, catalytic subunit
Cryptosporidium parvum cgd1_2360   protein phosphatase 4 (formerly X), catalytic subunit; Protein phosphatase 4, catalytic subunit
Dictyostelium discoideum DDB_G0272116   protein phosphatase 4 catalytic subunit
Drosophila melanogaster Dmel_CG32505   Protein phosphatase 19C
Echinococcus granulosus EgrG_001008700   serine:threonine protein phosphatase 4 catalytic
Entamoeba histolytica EHI_151480   serine/threonine-protein phosphatase 4 catalytic subunit, putative
Entamoeba histolytica EHI_051890   serine/threonine-protein phosphatase ppe1, putative
Entamoeba histolytica EHI_178880   serine/threonine-protein phosphatase ppe1, putative
Entamoeba histolytica EHI_147720   serine/threonine-protein phosphatase, putative
Entamoeba histolytica EHI_010290   serine/threonine-protein phosphatase, putative
Echinococcus multilocularis EmuJ_001008700   serine:threonine protein phosphatase 4 catalytic
Giardia lamblia GL50803_2053   Serine/threonine protein phosphatase 4
Homo sapiens ENSG00000149923   protein phosphatase 4, catalytic subunit
Leishmania braziliensis LbrM.28.2880   protein phosphatase 4 catalytic subunit, putative
Leishmania braziliensis LbrM.32.3330   protein phosphatase 2A catalytic subunit, putative
Leishmania donovani LdBPK_323230.1   protein phosphatase 2A catalytic subunit, putative
Leishmania donovani LdBPK_282870.1   protein phosphatase 4 catalytic subunit, putative
Leishmania infantum LinJ.32.3230   protein phosphatase 2A catalytic subunit, putative
Leishmania infantum LinJ.28.2870   protein phosphatase 4 catalytic subunit, putative
Leishmania major LmjF.32.3040   protein phosphatase 2A catalytic subunit, putative
Leishmania major LmjF.28.2670   protein phosphatase 4 catalytic subunit, putative
Leishmania mexicana LmxM.31.3040   protein phosphatase 2A catalytic subunit, putative
Leishmania mexicana LmxM.28.2670   protein phosphatase 4 catalytic subunit, putative
Loa Loa (eye worm) LOAG_09510   serine/threonine-protein phosphatase 4 catalytic subunit
Mus musculus ENSMUSG00000030697   protein phosphatase 4, catalytic subunit
Neospora caninum NCLIV_014260   hypothetical protein
Oryza sativa 4346619   Os09g0284200
Plasmodium berghei PBANKA_0828500   serine/threonine protein phosphatase 4
Plasmodium falciparum PF3D7_0927700   serine/threonine protein phosphatase 4, putative
Plasmodium knowlesi PKNH_0726100   serine/threonine protein phosphatase 4, putative
Plasmodium vivax PVX_099825   serine/threonine protein phosphatase 4, putative
Plasmodium yoelii PY00448   serine/threonine protein phosphatase pp-x isozyme 2
Saccharomyces cerevisiae YDR075W   phosphoprotein phosphatase PP4 catalytic subunit PPH3
Schistosoma japonicum Sjp_0055890   ko:K01090 protein phosphatase [EC3.1.3.16], putative
Schistosoma japonicum Sjp_0133830   Serine/threonine-protein phosphatase 4 catalytic subunit, putative
Schistosoma mansoni Smp_030710   protein phsophatase-2a
Schmidtea mediterranea mk4.003100.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.003629.01   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.000270.02   Protein phosphatase,putative
Schmidtea mediterranea mk4.002193.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.003158.01   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.022419.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.018257.01   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.018001.01   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.002486.01   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.000050.16   Protein phosphatase,putative
Schmidtea mediterranea mk4.036803.01  
Schmidtea mediterranea mk4.036506.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.018257.00  
Schmidtea mediterranea mk4.022022.01   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.015531.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.000473.00   Serine/threonine-protein phosphatase 4 catalytic subunit 1
Schmidtea mediterranea mk4.012995.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.007310.04   Protein phosphatase,putative
Schmidtea mediterranea mk4.028944.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.011112.00   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.025254.01   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.002809.03   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.012917.02   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.025499.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.009738.00   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.007403.06   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.002306.01   Protein phosphatase,putative
Schmidtea mediterranea mk4.015977.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.008288.03   Protein phosphatase,putative
Schmidtea mediterranea mk4.007403.05  
Schmidtea mediterranea mk4.009163.02   Protein phosphatase,putative
Schmidtea mediterranea mk4.004919.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.009872.00   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.003010.09   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.002098.03   Protein phosphatase,putative
Schmidtea mediterranea mk4.007390.01   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.013505.01   Protein phosphatase,putative
Schmidtea mediterranea mk4.004625.01   Protein phosphatase,putative
Schmidtea mediterranea mk4.005651.05   Protein phosphatase,putative
Schmidtea mediterranea mk4.019461.00   Protein phosphatase,putative
Schmidtea mediterranea mk4.002737.03   Serine/threonine-protein phosphatase 4 catalytic subunit
Schmidtea mediterranea mk4.009416.00   Serine/threonine-protein phosphatase 4 catalytic subunit
Trypanosoma brucei gambiense Tbg972.11.13400   protein phosphatase 4 catalytic subunit, putative
Trypanosoma brucei gambiense Tbg972.11.19160   protein phosphatase 2A catalytic subunit,serine/threonine protein phosphatase, putative
Trypanosoma brucei Tb927.11.16970   protein phosphatase 4, putative
Trypanosoma brucei Tb927.11.11940   protein phosphatase 2A, putative
Trypanosoma congolense TcIL3000.11.16890   protein phosphatase 4, putative
Trypanosoma congolense TcIL3000.11.12600   protein phosphatase 2A, putative
Trypanosoma cruzi TcCLB.511021.10   protein phosphatase 2A catalytic subunit, putative
Trypanosoma cruzi TcCLB.511211.60   protein phosphatase 4 catalytic subunit, putative
Trypanosoma cruzi TcCLB.511537.40   protein phosphatase 2A catalytic subunit, putative
Toxoplasma gondii TGME49_286210   protein phosphatase 4, catalytic subunit
Theileria parva TP02_0042   serine/threonine protein phosphatase, putative
Trichomonas vaginalis TVAG_459440   protein phosphatase-2B, putative
Trichomonas vaginalis TVAG_192950   protein phosphatase-5, putative

Essentiality

Tb927.11.16970 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.3770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.3770 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.3770 Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.01.3770 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Tb11.01.8740 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.8740 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb11.01.8740 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb11.01.8740 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_Y75B8A.30 Caenorhabditis elegans embryonic lethal wormbase
CELE_Y75B8A.30 Caenorhabditis elegans larval arrest wormbase
CELE_Y49E10.3 Caenorhabditis elegans larval lethal wormbase
PBANKA_0828500 Plasmodium berghei Dispensable plasmo
TGME49_286210 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Trypanosoma cruzi serine/threonine-protein phosphatase PP1 alpha 297 aa 44.2% 269 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative 294 aa 43.5% 269 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative 159 aa 43.8% 153 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1 alpha 169 aa 44.6% 168 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative 294 aa 43.5% 269 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative 294 aa 43.5% 269 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

  • Assay for Calcineurin (3.1.3.16 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.
  • BRENDA Assay
  • An enzyme with this EC number or name or sequence has been assayed in Trypanosoma brucei ( 1 )

Reagent availability

  • Reagent:
  • Target Type Source Notes
    Tb927.11.16970 purified protein BRENDA A protein with this EC number or name or sequence has been purified from Trypanosoma brucei ( 1 )

Bibliographic References

23 literature references were collected for this gene.

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Gene identifier Tb927.11.16970 (Trypanosoma brucei), protein phosphatase 4, putative
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