Detailed view for Tb927.1.1270

Basic information

TDR Targets ID: 15461
Trypanosoma brucei, homocysteine S-methyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 4.8667 | Length (AA): 433 | MW (Da): 48278 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02574   Homocysteine S-methyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0000096   sulfur amino acid metabolic process  
GO:0008898   homocysteine S-methyltransferase activity  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128779)

Species Accession Gene Product
Arabidopsis thaliana AT3G25900   homocysteine S-methyltransferase 1
Arabidopsis thaliana AT3G22740   homocysteine S-methyltransferase 3
Arabidopsis thaliana AT3G63250   homocysteine S-methyltransferase 2
Brugia malayi Bm1_40665   probable homocysteine S-methyltransferase
Candida albicans CaO19.386   S-adenosylmethionine:homocysteine S-methyltransferase
Candida albicans CaO19.8016   S-adenosylmethionine:homocysteine S-methyltransferase
Caenorhabditis elegans CELE_T13G4.4   Protein T13G4.4
Drosophila melanogaster Dmel_CG10623   CG10623 gene product from transcript CG10623-RC
Drosophila melanogaster Dmel_CG10621   CG10621 gene product from transcript CG10621-RA
Escherichia coli b0261   CP4-6 prophage
Leishmania braziliensis LbrM.35.6610   homocysteine S-methyltransferase, putative
Leishmania donovani LdBPK_366570.1   homocysteine S-methyltransferase, putative
Leishmania major LmjF.36.6310   homocysteine S-methyltransferase, putative
Leishmania mexicana LmxM.36.6310   homocysteine S-methyltransferase, putative
Loa Loa (eye worm) LOAG_05038   hypothetical protein
Mycobacterium leprae ML1478   PROBABLE HOMOCYSTEINE S-METHYLTRANSFERASE MMUM (S-METHYLMETHIONINE:HOMOCYSTEINE METHYLTRANSFERASE) (CYSTEINE METHYLTRANSFERASE)
Mycobacterium tuberculosis Rv2458   Probable homocysteine S-methyltransferase MmuM (S-methylmethionine:homocysteine methyltransferase) (cysteine methyltransferase)
Neospora caninum NCLIV_028500   GJ17676, related
Oryza sativa 4348656   Os10g0422200
Oryza sativa 4352747   Os12g0607000
Oryza sativa 4332095   Os03g0221200
Oryza sativa 4324713   Os01g0772900
Onchocerca volvulus OVOC5306  
Saccharomyces cerevisiae YPL273W   Sam4p
Saccharomyces cerevisiae YLL062C   Mht1p
Schistosoma japonicum Sjp_0063340   ko:K00547 homocysteine S-methyltransferase [EC2.1.1.10], putative
Schistosoma japonicum Sjp_0063350   Transmembrane protein 185B, putative
Schistosoma mansoni Smp_147610   hypothetical protein
Schmidtea mediterranea mk4.001783.04  
Trypanosoma brucei gambiense Tbg972.1.590   homocysteine S-methyltransferase, putative
Trypanosoma brucei Tb927.1.1270   homocysteine S-methyltransferase, putative
Trypanosoma congolense TcIL3000_0_17710   homocysteine S-methyltransferase, putative
Trypanosoma congolense TcIL3000_1_760   homocysteine S-methyltransferase, putative
Trypanosoma cruzi TcCLB.506479.120   cytidine triphosphate synthase, putative
Trypanosoma cruzi TcCLB.508993.20   homocysteine S-methyltransferase, putative
Toxoplasma gondii TGME49_257750   homocysteine s-methyltransferase domain-containing protein

Essentiality

Tb927.1.1270 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.1.1270 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.1.1270 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.1.1270 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.1270 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b0261 Escherichia coli non-essential goodall
TGME49_257750 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) decreased time (PATO:0000716) single cell organism (CARO:0000064) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell morphogenesis (GO:0000902) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
regulation of cell motility (GO:0051270) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell cycle (GO:0007049) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
mitochondrion organization and biogenesis (GO:0007005) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
regulation of cell motility (GO:0051270) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
endocytosis (GO:0006897) normal (PATO:0000461) bloodstream stage (PLO:0040) inferred from RNAi experiment (ECO:0000019) Trypanosoma brucei brucei No drug identifiers listed for this gene.
Annotator: ts4@sanger.ac.uk. Comment: . References: 16963636
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0072 0.2575 0.3535
0.0053 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.1.1270 (Trypanosoma brucei), homocysteine S-methyltransferase, putative
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