Detailed view for Tb927.5.2280

Basic information

TDR Targets ID: 15970
Trypanosoma brucei, acetyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.0776 | Length (AA): 434 | MW (Da): 49808 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 4

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01553   Acyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016746   transferase activity, transferring acyl groups  
GO:0008415   acyltransferase activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128431)

Species Accession Gene Product
Arabidopsis thaliana AT3G18850   lysophosphatidyl acyltransferase 5
Arabidopsis thaliana AT1G75020   lysophosphatidyl acyltransferase 4
Brugia malayi Bm1_55355   Protein FAM34A
Brugia malayi Bm1_04260   Hypothetical 45.9 kDa protein in KCS1-GCV1 intergenic region
Candida albicans CaO19.7781   possible role in phospholipid biosynthesis
Candida albicans CaO19.137   possible role in phospholipid biosynthesis
Caenorhabditis elegans CELE_T05H4.1   Protein ACL-8
Caenorhabditis elegans CELE_F55A11.5   Protein BUS-18
Dictyostelium discoideum DDB_G0269848   hypothetical protein
Echinococcus granulosus EgrG_000498400   lysocardiolipin acyltransferase 1
Echinococcus multilocularis EmuJ_000498400   lysocardiolipin acyltransferase 1
Homo sapiens ENSG00000172954   lysocardiolipin acyltransferase 1
Leishmania braziliensis LbrM.17.0930   hypothetical protein, conserved
Leishmania donovani LdBPK_171010.1   acyltransferase, putative
Leishmania infantum LinJ.17.1010   hypothetical protein, conserved
Leishmania major LmjF.17.0910   hypothetical protein, conserved
Leishmania mexicana LmxM.17.0910   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_05545   hypothetical protein
Loa Loa (eye worm) LOAG_04170   hypothetical protein
Loa Loa (eye worm) LOAG_10647   ACL-8 protein
Mus musculus ENSMUSG00000054469   lysocardiolipin acyltransferase 1
Oryza sativa 4327848   Os01g0782500
Oryza sativa 4339242   Os05g0502200
Onchocerca volvulus OVOC7311  
Saccharomyces cerevisiae YBR042C   Cst26p
Saccharomyces cerevisiae YDR018C   putative acyltransferase
Schistosoma japonicum Sjp_0309360   ko:K00655 1-acyl-sn-glycerol-3-phosphate acyltransferase [EC2.3.1.51], putative
Schistosoma mansoni Smp_095580   1-acylglycerol-3-phosphate acyltransferase
Schmidtea mediterranea mk4.002084.15   Putative 1-acylglycerol-3-phosphate acyltransferase
Trypanosoma brucei gambiense Tbg972.5.3210   acetyltransferase, putative
Trypanosoma brucei Tb927.5.2280   acetyltransferase, putative
Trypanosoma congolense TcIL3000_5_2060   acetyltransferase, putative
Trypanosoma cruzi TcCLB.511315.20   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.511755.50   hypothetical protein, conserved

Essentiality

Tb927.5.2280 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.2280 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.2280 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.2280 this record Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.5.2280 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.1

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0006 0.5 0.5
0.0006 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

12 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb927.5.2280 (Trypanosoma brucei), acetyltransferase, putative
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