Detailed view for Tb927.11.1370

Basic information

TDR Targets ID: 16102
Trypanosoma brucei, Dithiol glutaredoxin 1
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.7004 | Length (AA): 95 | MW (Da): 10576 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00462   Glutaredoxin

Gene Ontology

Mouse over links to read term descriptions.
GO:0005739   mitochondrion  
GO:0015035   protein disulfide oxidoreductase activity  
GO:0009055   electron carrier activity  
GO:0045454   cell redox homeostasis  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 95 2myg (A) 3 97 99.99 0 1 2.2651 -1.7

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 2MYG:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_126864)

Species Accession Gene Product
Arabidopsis thaliana AT5G63030   glutaredoxin-C1
Arabidopsis thaliana AT5G20500   glutaredoxin-C4
Arabidopsis thaliana AT1G77370   glutaredoxin-C3
Arabidopsis thaliana AT5G40370   glutaredoxin-C2
Babesia bovis BBOV_IV004320   glutaredoxin-like protein, putative
Brugia malayi Bm1_48585   Glutaredoxin family protein
Brugia malayi Bm1_08085   Glutaredoxin family protein
Candida albicans CaO19.6510   potential glutaredoxin similar to S. cerevisiae GRX1 (YCL035C)
Candida albicans CaO19.6059   one of two potential glutaredoxin genes similar to S. cerevisiae TTR1 (YDR513W) mitochondrial glutaredoxin
Candida albicans CaO19.6509   potential glutaredoxin similar to plant glutaredoxins
Candida albicans CaO19.13863   potential glutaredoxin similar to S. cerevisiae GRX1 (YCL035C)
Candida albicans CaO19_6510   hypothetical protein
Candida albicans CaO19_6509   hypothetical protein
Candida albicans CaO19.13480   one of two potential glutaredoxin genes similar to S. cerevisiae TTR1 (YDR513W) mitochondrial glutaredoxin
Candida albicans CaO19.13862   potential glutaredoxin similar to plant glutaredoxins
Caenorhabditis elegans CELE_ZK121.1   Protein GLRX-21, isoform B
Caenorhabditis elegans CELE_Y34D9A.6   Protein GLRX-10
Cryptosporidium hominis Chro.20270   glutaredoxin
Cryptosporidium parvum cgd2_2540   glutaredoxin related protein
Dictyostelium discoideum DDB_G0290015   hypothetical protein
Dictyostelium discoideum DDB_G0271084   hypothetical protein
Drosophila melanogaster Dmel_CG7975   CG7975 gene product from transcript CG7975-RA
Drosophila melanogaster Dmel_CG6852   CG6852 gene product from transcript CG6852-RC
Escherichia coli b3610   glutaredoxin 3
Echinococcus granulosus EgrG_000124800   glutaredoxin 1
Echinococcus granulosus EgrG_000226900   glutaredoxin
Echinococcus multilocularis EmuJ_000124800   glutaredoxin 1
Echinococcus multilocularis EmuJ_000226900   glutaredoxin
Homo sapiens ENSG00000173221   glutaredoxin (thioltransferase)
Homo sapiens ENSG00000023572   glutaredoxin 2
Leishmania braziliensis LbrM.27.0881   glutaredoxin-like protein, putative
Leishmania donovani LdBPK_270670.1   glutaredoxin-like protein
Leishmania infantum LinJ.27.0670   glutaredoxin-like protein
Leishmania major LmjF.27.0810   glutaredoxin-like protein
Leishmania mexicana LmxM.27.0810   glutaredoxin-like protein
Loa Loa (eye worm) LOAG_05670   hypothetical protein
Loa Loa (eye worm) LOAG_11828   hypothetical protein
Loa Loa (eye worm) LOAG_13565   hypothetical protein
Mus musculus ENSMUSG00000018196   glutaredoxin 2 (thioltransferase)
Mus musculus ENSMUSG00000021591   glutaredoxin
Neospora caninum NCLIV_045900   hypothetical protein
Neospora caninum NCLIV_038390   hypothetical protein
Oryza sativa 4330145   Os02g0646400
Oryza sativa 4329999   Os02g0618100
Oryza sativa 4336354   Os04g0508300
Plasmodium berghei PBANKA_0404700   glutaredoxin 1, putative
Plasmodium falciparum PF3D7_0306300   glutaredoxin 1
Plasmodium knowlesi PKNH_0836400   glutaredoxin 1, putative
Plasmodium vivax PVX_119440   glutaredoxin 1, putative
Plasmodium yoelii PY05305   thioltransferase
Saccharomyces cerevisiae YCL035C   dithiol glutaredoxin GRX1
Saccharomyces cerevisiae YDR513W   dithiol glutaredoxin GRX2
Schmidtea mediterranea mk4.000383.06  
Schmidtea mediterranea mk4.000054.17  
Trypanosoma brucei gambiense Tbg972.1.990   glutaredoxin, putative
Trypanosoma brucei gambiense Tbg972.11.1450   glutaredoxin, putative
Trypanosoma brucei Tb927.1.1770   Dithiol glutaredoxin 2
Trypanosoma brucei Tb927.11.1370   Dithiol glutaredoxin 1
Trypanosoma cruzi TcCLB.506475.116   glutaredoxin, putative
Trypanosoma cruzi TcCLB.511431.40   glutaredoxin, putative
Toxoplasma gondii TGME49_227150   glutaredoxin, putative
Toxoplasma gondii TGME49_279400   glutaredoxin, putative
Theileria parva TP01_0320   glutaredoxin-like protein
Wolbachia endosymbiont of Brugia malayi Wbm0415   glutaredoxin

Essentiality

Tb927.11.1370 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.47.0012 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb11.47.0012 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb11.47.0012 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb11.47.0012 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.1.1770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.1.1770 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.1.1770 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.1.1770 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
b3610 Escherichia coli non-essential goodall
PBANKA_0404700 Plasmodium berghei Dispensable plasmo
TGME49_227150 Toxoplasma gondii Probably non-essential sidik
TGME49_279400 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.11.1370 (Trypanosoma brucei), Dithiol glutaredoxin 1
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