Detailed view for Tb927.10.3100

Basic information

TDR Targets ID: 17361
Trypanosoma brucei, glycerol-3-phosphate acyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 9.0397 | Length (AA): 698 | MW (Da): 78195 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 3

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01553   Acyltransferase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016746   transferase activity, transferring acyl groups  
GO:0008415   acyltransferase activity  
GO:0008152   metabolic process  

Structural information

Modbase 3D models:

There is 1 model calculated for this protein. More info on this model, including the model itself is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
47 190 2dwc (A) 200 326 30.00 0.88 0.06 0.272004 0.73

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130377)

Species Accession Gene Product
Babesia bovis BBOV_IV005200   glycerol-3-phosphate-acyltransferase, putative
Candida albicans CaO19.5815   similar to S. cerevisiae GPT2 (YKR067W) Glycerol-3-phosphate acyltransferase involved in phospholipid biosynthesis, aka GAT1
Candida albicans CaO19.1289   similar to S. cerevisiae SCT1 (YBL011W) glycerol-3-phosphate acyltransferase that preferentially acts on 16-carbon fatty acids,
Candida albicans CaO19.13237   similar to S. cerevisiae GPT2 (YKR067W) Glycerol-3-phosphate acyltransferase involved in phospholipid biosynthesis, aka GAT1
Cryptosporidium hominis Chro.60161   glycerol-3-phosphate acyltransferase
Cryptosporidium parvum cgd6_1270   SCT1/Gpt2p-like glycerol-3-phosphate acyltransferase signal peptide plus 3 transmembrane domain
Leishmania braziliensis LbrM.03.0090   glycerol-3-phosphate acyltransferase, putative
Leishmania donovani LdBPK_030070.1   glycerol-3-phosphate acyltransferase, putative
Leishmania infantum LinJ.03.0070   glycerol-3-phosphate acyl transferase
Leishmania major LmjF.03.0080   glycerol-3-phosphate acyl transferase
Leishmania mexicana LmxM.03.0080   glycerol-3-phosphate acyl transferase
Neospora caninum NCLIV_029980   glycerol-3-phosphate acyltransferase, putative
Plasmodium berghei PBANKA_1428500   glycerol-3-phosphate 1-O-acyltransferase, putative
Plasmodium falciparum PF3D7_1212500   glycerol-3-phosphate 1-O-acyltransferase
Plasmodium knowlesi PKNH_1431900   glycerol-3-phosphate 1-O-acyltransferase, putative
Plasmodium vivax PVX_123330   glycerol-3-phosphate 1-O-acyltransferase, putative
Plasmodium yoelii PY06015   glycerol-3-phosphate acyltransferase
Saccharomyces cerevisiae YBL011W   bifunctional glycerol-3-phosphate/glycerone-phosphate O-acyltransferase SCT1
Trypanosoma brucei gambiense Tbg972.10.3920   glycerol-3-phosphate acyltransferase, putative
Trypanosoma brucei Tb927.10.3100   glycerol-3-phosphate acyltransferase, putative
Trypanosoma congolense TcIL3000_10_2550   glycerol-3-phosphate acyltransferase, putative
Trypanosoma cruzi TcCLB.510943.150   glycerol-3-phosphate acyltransferase, putative
Trypanosoma cruzi TcCLB.504867.90   glycerol-3-phosphate acyltransferase, putative
Toxoplasma gondii TGME49_256980   glycerol-3-phosphate-acyltransferase
Theileria parva TP01_0222   glycerol-3-phosphate o-acyltransferase, putative

Essentiality

Tb927.10.3100 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.3100 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.3100 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.3100 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.3100 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1428500 Plasmodium berghei Essential plasmo
TGME49_256980 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0006 0.5 0.5
0.0006 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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Gene identifier Tb927.10.3100 (Trypanosoma brucei), glycerol-3-phosphate acyltransferase, putative
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