Detailed view for Tb927.5.490

Basic information

TDR Targets ID: 17702
Trypanosoma brucei, methyltransferase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.2997 | Length (AA): 925 | MW (Da): 99929 | Paralog Number: 1

Signal peptide: Y | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01170   Putative RNA methylase family UPF0020

Gene Ontology

Mouse over links to read term descriptions.
GO:0008168   methyltransferase activity  
GO:0003676   nucleic acid binding  
GO:0032259   methylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_128028)

Species Accession Gene Product
Arabidopsis thaliana AT3G26410   tRNA modification 11 protein
Babesia bovis BBOV_IV002880   RNA methylase, putative
Brugia malayi Bm1_15325   hypothetical protein
Candida albicans CaO19.5987   potential SAM-dependent RNA methylase similar to S. cerevisiae YOL124C
Candida albicans CaO19.13408   potential SAM-dependent RNA methylase similar to S. cerevisiae YOL124C
Caenorhabditis elegans CELE_Y71F9AL.1   Protein Y71F9AL.1
Cryptosporidium hominis Chro.50400   hypothetical protein
Cryptosporidium parvum cgd5_4420   MJ0710-like (thump + methylase domains). RNA methylase
Dictyostelium discoideum DDB_G0283969   tRNA guanosine-2'-O-methyltransferase 11
Drosophila melanogaster Dmel_CG1074   CG1074 gene product from transcript CG1074-RA
Echinococcus granulosus EgrG_000926200   tRNA guanosine 2' O methyltransferase TRM11
Entamoeba histolytica EHI_023130   hypothetical protein, conserved
Echinococcus multilocularis EmuJ_000926200   tRNA guanosine 2' O methyltransferase TRM11
Giardia lamblia GL50803_13525   RNA methylase, putative
Homo sapiens ENSG00000066651   tRNA methyltransferase 11 homolog (S. cerevisiae)
Leishmania braziliensis LbrM.05.1010   methyltransferase-like protein
Leishmania donovani LdBPK_051010.1   methyltransferase-like protein
Leishmania infantum LinJ.05.1010   methyltransferase-like protein
Leishmania major LmjF.05.1010   methyltransferase-like protein
Leishmania mexicana LmxM.05.1010   methyltransferase-like protein
Loa Loa (eye worm) LOAG_07725   hypothetical protein
Mus musculus ENSMUSG00000019792   tRNA methyltransferase 11
Neospora caninum NCLIV_068140   Methyltransferase-like protein, related
Oryza sativa 4329664   Os02g0556400
Plasmodium berghei PBANKA_1356200   tRNA guanosine-2'-O-methyltransferase, putative
Plasmodium falciparum PF3D7_1343200   tRNA guanosine-2'-O-methyltransferase, putative
Plasmodium knowlesi PKNH_1258200   tRNA guanosine-2'-O-methyltransferase, putative
Plasmodium vivax PVX_083055   hypothetical protein, conserved
Plasmodium yoelii PY05490   hypothetical protein
Saccharomyces cerevisiae YOL124C   Trm11p
Schistosoma japonicum Sjp_0020400   ko:K00599 TRMT11, C6orf75; tRNA methyltransferase 11 homolog [EC:2.1.1.-], putative
Schistosoma mansoni Smp_077670   hypothetical protein
Trypanosoma brucei gambiense Tbg972.5.500   methyltransferase, putative
Trypanosoma brucei Tb11.v5.0219   methyltransferase, putative
Trypanosoma brucei Tb927.5.490   methyltransferase, putative
Trypanosoma congolense TcIL3000_5_110   methyltransferase, putative
Trypanosoma cruzi TcCLB.509885.60   methyltransferase, putative
Trypanosoma cruzi TcCLB.508397.60   methyltransferase, putative
Toxoplasma gondii TGME49_277860   hypothetical protein
Theileria parva TP01_0635   hypothetical protein, conserved
Trichomonas vaginalis TVAG_299110   S.cerevisiae chromosome XV reading frame orf yol124c, putative

Essentiality

Tb927.5.490 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.490 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.490 this record Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.5.490 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.490 this record Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1356200 Plasmodium berghei Slow plasmo
TGME49_277860 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

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User comments

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Gene identifier Tb927.5.490 (Trypanosoma brucei), methyltransferase, putative
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