Detailed view for Tb11.v5.0409

Basic information

TDR Targets ID: 18101
Trypanosoma brucei, guanine deaminase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 6.4527 | Length (AA): 516 | MW (Da): 55322 | Paralog Number: 1

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 0

Druggability Group : DG4

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01979   Amidohydrolase family

Gene Ontology

Mouse over links to read term descriptions.
GO:0016810   hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds  
GO:0006147   guanine catabolic process  
GO:0016787   hydrolase activity  
GO:0008892   guanine deaminase activity  
GO:0008270   zinc ion binding  

Metabolic Pathways

Purine metabolism (KEGG)

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
NA% percentile Procyclic. Siegel TN
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_127281)

Species Accession Gene Product
Candida albicans CaO19.7029   putative guanine deaminase
Candida albicans CaO19_7029   hypothetical protein
Dictyostelium discoideum DDB_G0285467   metal-dependent hydrolase family protein
Dictyostelium discoideum DDB_G0277743   guanine deaminase
Drosophila melanogaster Dmel_CG18143   Dihydropterin deaminase
Escherichia coli b2879   putative chlorohydrolase/aminohydrolase
Escherichia coli b2883   guanine deaminase
Entamoeba histolytica EHI_151430   guanine deaminase, putative
Homo sapiens ENSG00000119125   guanine deaminase
Leishmania braziliensis LbrM.29.0910   guanine deaminase, putative
Leishmania donovani LdBPK_290920.1   guanine deaminase, putative
Leishmania infantum LinJ.29.0920   guanine deaminase, putative
Leishmania major LmjF.29.0867   guanine deaminase, putative
Leishmania mexicana LmxM.08_29.0867   guanine deaminase, putative
Mus musculus ENSMUSG00000058624   guanine deaminase
Mycobacterium ulcerans MUL_2914   hydroxydechloroatrazine ethylaminohydrolase
Oryza sativa 4352197   Os12g0468600
Saccharomyces cerevisiae YDL238C   guanine deaminase
Schmidtea mediterranea mk4.034622.03  
Trypanosoma brucei gambiense Tbg972.5.6150   guanine deaminase, putative,guanase, putative
Trypanosoma brucei Tb05.5K5.200   guanine deaminase, putative
Trypanosoma brucei Tb11.v5.0409   guanine deaminase, putative
Trypanosoma cruzi TcCLB.504431.100   guanine deaminase, putative
Trypanosoma cruzi TcCLB.507951.150   guanine deaminase, putative
Trichomonas vaginalis TVAG_029040   guanine deaminase, putative
Trichomonas vaginalis TVAG_211590   protein ssnA, putative
Trichomonas vaginalis TVAG_228260   protein ssnA, putative

Essentiality

Tb11.v5.0409 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.5.4560 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.5.4560 this record Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.5.4560 this record Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.5.4560 this record Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
b2879 Escherichia coli non-essential goodall
b2883 Escherichia coli non-essential goodall
Show/Hide essentiality data references
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 3 days). References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) normal (PATO:0000461) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Trypanosoma cruzi guanine deaminase, putative Compounds References
Trypanosoma cruzi guanine deaminase, putative Compounds References
Homo sapiens guanine deaminase Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0157 0.2975 0.5
0.0132 0.457 1
0.0132 0.7422 1
0.0264 0.2942 0.2905
0.0132 0.457 1
0.0132 0.457 1
0.0157 0.3576 0.5076
0.0142 0.4241 1
0.0277 0.2931 0.2924
0.0126 0.9487 1
0.0157 0.9506 1
0.0252 0.2848 0.2812
0.0157 1 1
0.0132 0.3951 1
0.0157 0.3576 0.5076
0.0134 0.4949 1
0.0136 0.5823 1
0.0157 1 1
0.0151 0.4644 1
0.0118 0.4869 0.5
0.0132 0.7422 1
0.0132 0.3951 1

Assayability

Assay information

  • Assay for Guanase (3.5.4.3 ) Sigma-Aldrich
  • Automatic link to known assays based on EC numbers.

Reagent availability

No reagent availability information for this target.

Bibliographic References

4 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier Tb11.v5.0409 (Trypanosoma brucei), guanine deaminase, putative
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