Detailed view for Tb927.7.3900

Basic information

TDR Targets ID: 19570
Trypanosoma brucei, vacuolar transporter chaperone, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.6779 | Length (AA): 180 | MW (Da): 19768 | Paralog Number: 0

Signal peptide: N | GPI Anchor: | Predicted trans-membrane segments: 3

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF02656   Domain of unknown function (DUF202)

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

No model available for this protein in Modbase.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile Procyclic, Bloodstream Form. Siegel TN
Show/Hide expression data references
  • Siegel TN Genome-wide analysis of mRNA abundance in two life-cycle stages of Trypanosoma brucei and identification of splicing and polyadenylation sites.

Orthologs

Ortholog group members (OG5_130969)

Species Accession Gene Product
Candida albicans CaO19.5455   similar to S. cerevisiae vacuolar transporter chaperone VTC1 and to S. pombe negative regulator of cdc42 GTPase NRF1
Candida albicans CaO19.12910   similar to S. cerevisiae vacuolar transporter chaperone VTC1 and to S. pombe negative regulator of cdc42 GTPase NRF1
Cryptosporidium hominis Chro.60386   G-protein associated signal transduction protein
Cryptosporidium parvum cgd6_3330   membrane associated protein with a SPX domain (SYG1, Pho81 and XPR1) domain at the N-terminus and a vtc1p domain at the C-termin
Dictyostelium discoideum DDB_G0277625   hypothetical protein
Dictyostelium discoideum DDB_G0287759   hypothetical protein
Leishmania braziliensis LbrM.14.1020   hypothetical protein, conserved
Leishmania donovani LdBPK_141100.1   Domain of unknown function (DUF202), putative
Leishmania infantum LinJ.14.1100   hypothetical protein, conserved
Leishmania major LmjF.14.1040   hypothetical protein, conserved
Leishmania mexicana LmxM.14.1040   hypothetical protein, conserved
Plasmodium berghei PBANKA_1444900   vacuolar transporter chaperone, putative
Plasmodium knowlesi PKNH_1449600   vacuolar transporter chaperone, putative
Plasmodium vivax PVX_124150   hypothetical protein, conserved
Plasmodium yoelii PY03770   possible negative regulator of cdc42p-related
Saccharomyces cerevisiae YER072W   Vtc1p
Trypanosoma brucei gambiense Tbg972.7.4360   vacuolar transporter chaperone, putative
Trypanosoma brucei Tb927.7.3900   vacuolar transporter chaperone, putative
Trypanosoma congolense TcIL3000_0_36420   vacuolar transporter chaperone, putative
Trypanosoma cruzi TcCLB.511249.44   vacuolar transporter chaperone, putative
Toxoplasma gondii TGME49_298630   vacuolar transporter chaperone VTC2

Essentiality

Tb927.7.3900 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.3900 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.3900 this record Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.7.3900 this record Trypanosoma brucei significant gain of fitness in procyclic forms alsford
Tb927.7.3900 this record Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1444900 Plasmodium berghei Dispensable plasmo
TGME49_298630 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
cell proliferation (GO:0008283) normal (PATO:0000461) bloodstream stage trypomastigotes (PLO:0027) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: normal cell proliferation (no significant loss or gain of fitness) in bloodstream forms (stage 6 days). References: 21363968
cell proliferation (GO:0008283) increased (PATO:0000470) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: increased cell proliferation (significant gain of fitness) in procyclic forms . References: 21363968
cell proliferation (GO:0008283) decreased (PATO:0000468) procyclic (PLO:0034) inferred from RNAi experiment (ECO:0000019) No drug identifiers listed for this gene.
Annotator: fernan@iib.unsam.edu.ar. Comment: decreased cell proliferation (significant loss of fitness) in differentiation of procyclic to bloodstream forms . References: 21363968

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

2 literature references were collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Tb927.7.3900 (Trypanosoma brucei), vacuolar transporter chaperone, putative
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