Detailed view for PF3D7_1302600

Basic information

TDR Targets ID: 2008
Plasmodium falciparum, deoxyhypusine hydroxylase
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 4.7184 | Length (AA): 411 | MW (Da): 46451 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13646   HEAT repeats

Gene Ontology

Mouse over links to read term descriptions.
GO:0019135   deoxyhypusine monooxygenase activity  
GO:0008150   biological_process  
GO:0005575   cellular_component  
GO:0005488   binding  
GO:0008612   peptidyl-lysine modification to hypusine  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
2 411 1wa5 (B) 35 466 13.00 0 1 0.82 -0.06
9 410 1w63 (B) 17 427 15.00 0 0.97 1.15 -0.1
79 401 1jdh (A) 151 518 15.00 0.000000012 0.21 0.85 -0.38
41 403 4d4z (A) 4 283 34.00 0 1 1.04261 -0.16
37 403 4d4z (A) 0 283 33.00 0 1 0.989644 -0.34
42 186 4xvp (D) 11 149 32.00 0.00025 1 0.791798 -0.77
88 171 4jw3 (C) 20 103 35.00 0.012 1 0.71938 -1.13
88 171 3ltj (A) 25 108 36.00 0.0014 1 0.72338 -1.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile merozoite, sporozoite, early ring, early trophozoite, late ring, Ring. PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 48 hs, gametocyte, early schizont, late schizont, late trophozoite, Oocyst, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 40 hs, Female Gametocyte. Otto TD Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Male Gametocyte. Lasonder E
Show/Hide expression data references
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.

Orthologs

Ortholog group members (OG5_128124)

Species Accession Gene Product
Arabidopsis thaliana AT3G58180   ARM repeat superfamily protein
Babesia bovis BBOV_IV009880   PBS lyase HEAT-like repeat domain containing protein
Brugia malayi Bm1_56815   PBS lyase HEAT-like repeat family protein
Candida albicans CaO19.2286   similar to S. cerevisiae YJR070C
Candida albicans CaO19.9826   similar to S. cerevisiae YJR070C
Caenorhabditis elegans CELE_C14A4.1   Protein DOHH-1
Cryptosporidium hominis Chro.40055   hypothetical protein
Cryptosporidium parvum cgd4_410   protein with 4xEZ_heat domains
Dictyostelium discoideum DDB_G0272634   PBS lyase HEAT-like repeat-containing protein
Dictyostelium discoideum DDB_G0274079   PBS lyase HEAT-like repeat-containing protein
Drosophila melanogaster Dmel_CG2245   CG2245 gene product from transcript CG2245-RA
Echinococcus granulosus EgrG_000637700   deoxyhypusine hydroxylase:monooxygenase
Echinococcus multilocularis EmuJ_000637700   deoxyhypusine hydroxylase:monooxygenase
Homo sapiens ENSG00000129932   deoxyhypusine hydroxylase/monooxygenase
Leishmania braziliensis LbrM.26.1840   hypothetical protein, conserved
Leishmania donovani LdBPK_261920.1   hypothetical protein, conserved
Leishmania infantum LinJ.26.1920   hypothetical protein, conserved
Leishmania major LmjF.26.1910   hypothetical protein, conserved
Leishmania mexicana LmxM.26.1910   hypothetical protein, conserved
Mus musculus ENSMUSG00000078440   deoxyhypusine hydroxylase/monooxygenase
Neospora caninum NCLIV_042540   PBS lyase HEAT-like repeat domain-containing protein, putative
Oryza sativa 4351581   Os12g0163300
Oryza sativa 4352843   Os12g0626100
Plasmodium berghei PBANKA_1401100   deoxyhypusine hydroxylase, putative
Plasmodium falciparum PF3D7_1302600   deoxyhypusine hydroxylase
Plasmodium knowlesi PKNH_1402800   deoxyhypusine hydroxylase, putative
Plasmodium vivax PVX_121970   hypothetical protein, conserved
Plasmodium yoelii PY00741   PBS lyase HEAT-like repeat, putative
Saccharomyces cerevisiae YJR070C   Lia1p
Schistosoma mansoni Smp_056370   pbs lyase heat-like protein
Schmidtea mediterranea mk4.000111.12   Deoxyhypusine hydroxylase
Schmidtea mediterranea mk4.002413.02   Deoxyhypusine hydroxylase
Schmidtea mediterranea mk4.000111.08   Deoxyhypusine hydroxylase
Trypanosoma brucei gambiense Tbg972.9.1120   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.2580   deoxyhypusine hydroxylase, putative
Trypanosoma congolense TcIL3000_9_750   hypothetical protein, conserved
Trypanosoma cruzi TcCLB.507615.70   deoxyhypusine hydroxylase, putative
Trypanosoma cruzi TcCLB.508347.130   deoxyhypusine hydroxylase, putative
Theileria parva TP01_0705   hypothetical protein, conserved

Essentiality

PF3D7_1302600 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.160.1240 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb09.160.1240 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb09.160.1240 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.160.1240 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_C14A4.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_C14A4.1 Caenorhabditis elegans slow growth wormbase
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

Annotated phenotypes:

Affected Entity Phenotypic quality Occurs in Occurs at Evidence Observed in Drugs/Inhibitors
growth (GO:0040007) lethal (sensu genetics) (PATO:0000718) multi-cellular organism (CARO:0000012) bloodstream stage (PLO:0040) in vivo inhibition (TDR:00016) Plasmodium falciparum 365600   452837   503442   565762  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 16550432
catalytic activity (GO:0003824) decreased (PATO:0000468) in vitro (MI:0492) inferred from specific protein inhibition (ECO:0000020) Plasmodium falciparum 365600   452837   503442   565762  
Annotator: saralph@unimelb.edu.au. Comment: 012/Mar/09. References: 16550432

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

Compound Source Reference
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References
Curated by TDRTargets References

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

1 literature reference was collected for this gene.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier PF3D7_1302600 (Plasmodium falciparum), deoxyhypusine hydroxylase
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