Detailed view for PF3D7_1304500

Basic information

TDR Targets ID: 2016
Plasmodium falciparum, small heat shock protein, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 6.3589 | Length (AA): 211 | MW (Da): 25195 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00011   Hsp20/alpha crystallin family

Gene Ontology

Mouse over links to read term descriptions.
GO:0006986   response to unfolded protein  
GO:0009408   response to heat  

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
59 210 1gme (A) 7 149 29.00 0 1 0.95 0.77
87 210 3w1z (A) 20 142 30.00 0.00000011 1 0.968278 0.11
102 198 3gla (A) 42 136 35.00 0.0000022 1 0.994716 -1.16
117 197 4ydz (A) 55 126 40.00 0.0024 1 0.773186 -0.49

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, Oocyst. Otto TD Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile gametocyte, early schizont, early trophozoite, late trophozoite, Female Gametocyte. PlasmoDB Lasonder E
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, Ring, Male Gametocyte. Otto TD Zanghi G Lasonder E
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile Sporozoite. Zanghi G
Show/Hide expression data references
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.

Orthologs

Ortholog group members (OG5_126935)

Species Accession Gene Product
Arabidopsis thaliana AT4G27670   heat shock protein 21
Arabidopsis thaliana AT1G07400   class I heat shock protein
Arabidopsis thaliana AT3G46230   heat shock protein 17.4
Arabidopsis thaliana AT5G59720   heat shock protein 18.2
Arabidopsis thaliana AT1G59860   HSP20-like chaperone
Arabidopsis thaliana AT2G29500   HSP20 family protein
Arabidopsis thaliana AT1G53540   HSP20-like chaperone
Babesia bovis BBOV_IV001650   conserved hypothetical protein
Dictyostelium discoideum DDB_G0280215   heat shock protein Hsp20 domain-containing protein
Dictyostelium discoideum DDB_G0288921   heat shock protein Hsp20 domain-containing protein
Drosophila melanogaster Dmel_CG4460   Heat shock protein 22
Entamoeba histolytica EHI_125830   heat shock protein, Hsp20 family
Leishmania braziliensis LbrM.29.2420   heat shock protein 20, putative
Leishmania donovani LdBPK_292560.1   heat shock protein 20, putative
Leishmania infantum LinJ.29.2560   heat shock protein 20, putative
Leishmania major LmjF.29.2450   heat shock protein 20, putative
Leishmania mexicana LmxM.08_29.2450   heat shock protein 20, putative
Mycobacterium leprae ML1795   18 KDA antigen Hsp18 (HSP 16.7)
Mycobacterium tuberculosis Rv0251c   Heat shock protein Hsp (heat-stress-induced ribosome-binding protein A)
Mycobacterium tuberculosis Rv2031c   Heat shock protein HspX (alpha-crystallin homolog) (14 kDa antigen) (HSP16.3)
Mycobacterium ulcerans MUL_2232   molecular chaperone
Mycobacterium ulcerans MUL_1178   heat shock protein Hsp
Neospora caninum NCLIV_041850   Hsp20/alpha crystallin domain-containing protein, putative
Neospora caninum NCLIV_013940   Heat shock protein 17.4, related
Oryza sativa 4332363   Os03g0267200
Oryza sativa 4332237   Os03g0245800
Oryza sativa 4332361   Os03g0267000
Oryza sativa 4325694   Os01g0135800
Oryza sativa 4332357   Os03g0266300
Oryza sativa 4325698   Os01g0136200
Oryza sativa 4332360   Os03g0266900
Oryza sativa 9267020   Os05g0296650
Oryza sativa 4325696   Os01g0136000
Oryza sativa 4325697   Os01g0136100
Plasmodium falciparum PF3D7_1304500   small heat shock protein, putative
Plasmodium knowlesi PKNH_1404700   small heat shock protein, putative
Plasmodium vivax PVX_122065   heat shock protein, putative
Saccharomyces cerevisiae YBR072W   Hsp26p
Trypanosoma brucei gambiense Tbg972.3.3490   heat shock protein 20, putative
Trypanosoma brucei Tb927.3.3330   heat shock protein 20, putative
Trypanosoma cruzi TcCLB.508153.270   heat shock protein 20, putative
Trypanosoma cruzi TcCLB.510323.40   heat shock protein 20, putative
Toxoplasma gondii TGME49_286720   heat shock protein HSP28
Toxoplasma gondii TGME49_289600   heat shock protein HSP29
Trichomonas vaginalis TVAG_381290   heat shock protein, putative
Trichomonas vaginalis TVAG_197980   heat shock protein, putative
Trichomonas vaginalis TVAG_287530   heat shock protein, putative

Essentiality

PF3D7_1304500 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
mtu254 Mycobacterium tuberculosis non-essential nmpdr
mtu2063 Mycobacterium tuberculosis non-essential nmpdr
Tb927.3.3330 Trypanosoma brucei significant gain of fitness in bloodstream forms (3 days) alsford
Tb927.3.3330 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.3.3330 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.3.3330 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_289600 Toxoplasma gondii Probably non-essential sidik
TGME49_286720 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

  • Pkno006322AAA;
  • Type Source Notes
    soluble recombinant protein Structural Genomics for Pathogenic Protozoa (SGPP) Pkno006322; Recombinant protein: full-length; Source: P knowlesi; small heat shock protein, putative homolog ; Identifier: PKH_140300

Bibliographic References

No literature references available for this target.

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Gene identifier PF3D7_1304500 (Plasmodium falciparum), small heat shock protein, putative
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