Detailed view for LmjF.24.1450

Basic information

TDR Targets ID: 21414
Leishmania major, protein kinase, putative,NPK1-related protein kinase-like
Source Database / ID:  TriTrypDB  GeneDB

pI: 7.3564 | Length (AA): 601 | MW (Da): 63602 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 11 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
148 600 1fmk () 83 520 21.00 0 1 0.74 0.26
336 597 1cm8 (A) 41 312 26.00 0 1 0.48 -0.86
49 157 4yif (F) 26 141 25.00 0.74 0.18 0.475964 -0.49
219 590 3vs1 (A) 174 512 25.00 0 1 0.573868 0.78
309 536 5lpb (A) 875 1085 36.00 0 1 0.553968 0.43
325 533 2eu9 (A) 160 370 27.00 0.00000000049 0.97 0.397354 0.25
327 598 4bf2 (B) 685 940 39.00 0 1 0.765179 -0.15
372 596 2bva (A) 363 572 32.00 0 1 0.684976 -0.03
406 537 4ra4 (A) 417 538 40.00 0.000000000042 0.77 0.493234 0.21
422 533 4qpm (B) 887 1001 25.00 0.79 0.33 0.472956 -0.46
489 591 3kmu (A) 345 443 27.00 0.3 0.98 0.557981 -0.81

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_141398)

Species Accession Gene Product
Leishmania braziliensis LbrM.24.1410   protein kinase, putative,NPK1-related protein kinase-like
Leishmania donovani LdBPK_241500.1   protein kinase, putative
Leishmania infantum LinJ.24.1500   protein kinase, putative,NPK1-related protein kinase-like
Leishmania major LmjF.24.1450   protein kinase, putative,NPK1-related protein kinase-like
Leishmania mexicana LmxM.24.1450   protein kinase, putative,NPK1-related protein kinase-like
Trypanosoma brucei gambiense Tbg972.8.7000   protein kinase, putative
Trypanosoma brucei Tb927.8.6810   STE/STE11 serine/threonine-protein kinase, putative
Trypanosoma congolense TcIL3000_8_6720   protein kinase, putative
Trypanosoma cruzi TcCLB.507991.20   STE/STE11 serine/threonine-protein kinase, putative
Trypanosoma cruzi TcCLB.506205.40   STE/STE11 serine/threonine-protein kinase, putative

Essentiality

LmjF.24.1450 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.6810 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.6810 Trypanosoma brucei significant gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.6810 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.6810 Trypanosoma brucei significant gain of fitness in differentiation of procyclic to bloodstream forms alsford
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.5

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Rattus norvegicus Cell division protein kinase 5 292 aa 25.6% 305 aa Compounds References
Rattus norvegicus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 23.9% 322 aa Compounds References
Homo sapiens Cyclin-dependent kinase 1/cyclin B1 297 aa 28.2% 312 aa Compounds References
Rattus norvegicus Serine/threonine-protein kinase pim-3 326 aa 23.8% 302 aa Compounds References
Oryctolagus cuniculus Cyclin-dependent kinase 4 189 aa 31.5% 197 aa Compounds References
Plasmodium falciparum (isolate 3D7) Cell division control protein 2 homolog 288 aa 26.8% 295 aa Compounds References
Bos taurus cAMP-dependent protein kinase alpha-catalytic subunit 351 aa 24.2% 322 aa Compounds References
Patiria pectinifera Cdc2 300 aa 27.5% 313 aa Compounds References
Rattus norvegicus Jak1 protein 210 aa 27.7% 213 aa Compounds References
Schizosaccharomyces pombe 972h- Casein kinase II subunit alpha 332 aa 21.0% 305 aa Compounds References
Rattus norvegicus MAP kinase p38 alpha 360 aa 26.6% 305 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0091 1 0.5
0.0081 0.5 0.5
0.0004 0.5 0.5
0.0063 1 1
0.0033 0.5 0.5
0.0007 0.5 0.5
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0059 1 1
0.0069 0.3067 1
0.0066 0.3101 0
0.0067 0.5 0.5
0.0016 0.5 0.5
0.0027 1 0.5
0.0036 0.5 0.5
0.0039 0.5 0.5
0.0061 0.6883 0.5304
0.0026 0.5 0.5
0.0011 1 0.5
0.0092 1 0.5
0.0029 0.5 0.5
0.0008 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0059 1 1
0.0062 0.6935 0
0.0022 0.5 0.5
0.0059 1 1
0.0063 0.7244 0.2543
0.0064 0.3377 0
0.0056 1 0.5
0.0088 0.4477 1
0.0081 1 0.5
0.0093 0.8828 0
0.0032 0.5 0.5
0.0037 1 0.5
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0016 0.5 0.5
0.0033 1 1
0.0012 0.5 0.5
0.0007 0.5 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.24.1450 (Leishmania major), protein kinase, putative,NPK1-related protein kinase-like
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