Detailed view for LmjF.35.4710

Basic information

TDR Targets ID: 22828
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.6747 | Length (AA): 723 | MW (Da): 78826 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00012   Hsp70 protein

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
23 642 1yuw (A) 2 554 24.00 0 1 1.01 0.09
24 422 1s3x (A) 3 382 27.00 0 1 0.94 -1.1
28 635 1yuw (A) 7 547 28.00 0 1 1.04 0
24 706 3c7n (A) 1 647 25.00 0 1 1.14237 0.5
24 307 4czg (A) 9 224 31.00 0.069 0.93 0.265608 1.83
25 714 3d2f (A) 2 652 22.00 0.0000000059 1 1.09616 0.34
27 421 3gl1 (A) 9 384 29.00 0 1 0.885035 -0.29
623 703 3lof (A) 535 609 27.00 0 0.4 0.447733 -1.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128359)

Species Accession Gene Product
Arabidopsis thaliana AT4G16660   heat shock protein 70
Babesia bovis BBOV_I003640   DnaK family domain containing protein
Brugia malayi Bm1_39345   dnaK protein
Candida albicans CaO19.5830   translocation of protein precursors across ER
Candida albicans CaO19.13252   translocation of protein precursors across ER
Caenorhabditis elegans CELE_T14G8.3   Protein T14G8.3, isoform A
Caenorhabditis elegans CELE_T24H7.2   Protein T24H7.2
Cryptosporidium hominis Chro.20349   HSP protein
Cryptosporidium parvum cgd2_3330   APG-1 like HSP70 domain containing protein, signal peptide plus likely ER retention motif
Dictyostelium discoideum DDB_G0273093   heat shock protein 70 family member
Dictyostelium discoideum DDB_G0273813   heat shock protein 70 family member
Drosophila melanogaster Dmel_CG2918   CG2918 gene product from transcript CG2918-RB
Echinococcus granulosus EgrG_000981800   Heat shock protein Hsp70
Entamoeba histolytica EHI_148990   heat shock protein 70, putative
Echinococcus multilocularis EmuJ_000981800   Heat shock protein Hsp70
Homo sapiens ENSG00000149428   hypoxia up-regulated 1
Leishmania braziliensis LbrM.34.4680   hypothetical protein, conserved
Leishmania donovani LdBPK_354780.1   Hsp70 protein, putative
Leishmania infantum LinJ.35.4780   hypothetical protein, conserved
Leishmania major LmjF.35.4710   hypothetical protein, conserved
Leishmania mexicana LmxM.34.4710   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_02797   dnaK protein
Mus musculus ENSMUSG00000032115   hypoxia up-regulated 1
Neospora caninum NCLIV_046170   Heat Shock Protein 70, ER lumen, related
Oryza sativa 4330492   Os02g0710900
Plasmodium berghei PBANKA_1357200   heat shock protein 110, putative
Plasmodium falciparum PF3D7_1344200   heat shock protein 110, putative
Plasmodium knowlesi PKNH_1257200   heat shock protein 110, putative
Plasmodium vivax PVX_083105   heat shock protein 110, putative
Plasmodium yoelii PY05402   putative HSP protein
Saccharomyces cerevisiae YKL073W   Hsp70 family chaperone LHS1
Schistosoma japonicum Sjp_0060680   ko:K09486 hypoxia up-regulated 1, putative
Schistosoma mansoni Smp_088950   hypothetical protein
Schmidtea mediterranea mk4.000709.08   Hypoxia up-regulated protein 1
Schmidtea mediterranea mk4.000785.07   Hypoxia up-regulated protein 1
Trypanosoma brucei gambiense Tbg972.9.5670   hypothetical protein, conserved
Trypanosoma brucei Tb927.9.9860   170 kDa glucose-regulated protein, putative
Trypanosoma cruzi TcCLB.508457.20   Hsp70 protein, putative
Trypanosoma cruzi TcCLB.506885.440   Hsp70 protein, putative
Toxoplasma gondii TGME49_226830   DnaK family protein
Theileria parva TP01_0479   dnaK domain protein, putative

Essentiality

LmjF.35.4710 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb09.211.1390 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb09.211.1390 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb09.211.1390 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb09.211.1390 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
PBANKA_1357200 Plasmodium berghei Essential plasmo
TGME49_226830 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bos taurus Heat shock cognate 71 kDa protein 650 aa 23.8% 663 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.35.4710 (Leishmania major), hypothetical protein, conserved
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