Detailed view for Bm1_06985

Basic information

TDR Targets ID: 233899
Brugia malayi, rRNA methyltransferase
Source Database / ID:  GenBank

pI: 9.6067 | Length (AA): 789 | MW (Da): 90634 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01728   FtsJ-like methyltransferase
PF07780   Spb1 C-terminal domain
PF11861   Domain of unknown function (DUF3381)

Gene Ontology

Mouse over links to read term descriptions.
GO:0031167   rRNA methylation  
GO:0005634   nucleus  
GO:0008649   rRNA methyltransferase activity  
GO:0008168   methyltransferase activity  
GO:0003676   nucleic acid binding  
GO:0032259   methylation  
GO:0006364   rRNA processing  
GO:0001510   RNA methylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
10 211 4n49 (A) 218 460 23.00 0 1 0.34962 -0.29
23 203 1ej0 (A) 30 209 32.00 0 1 0.693404 -1.35
24 200 2nyu (A) 2 187 34.00 0 1 0.597935 -1.19
615 787 4uos (A) 6 179 9.00 0.025 0.01 0.363965 -1.11
681 785 2fxo (A) 841 946 10.00 0.16 0 0.20578 -0.3

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

No expression data available for this gene

Orthologs

Ortholog group members (OG5_126871)

Species Accession Gene Product
Arabidopsis thaliana AT4G25730   FtsJ-like methyltransferase family protein
Babesia bovis BBOV_III009210   ribosomal RNA large subunit methyltransferase J family protein
Babesia bovis BBOV_II002180   ribosomal RNA large subunit methyltransferase J protein, putative
Brugia malayi Bm1_19645   ribosomal RNA methyltransferase
Brugia malayi Bm1_06985   rRNA methyltransferase
Candida albicans CaO19.7601   similar to S. cerevisiae MRM2 (YGL136C) mitochondrial rRNA methyltransferase
Candida albicans CaO19.7727   potential FtsJ-like methyltransferase similar to S. cerevisiae SPB1 (YCL054W) putative rRNA methyltransferase
Candida albicans CaO19.76   potential FtsJ-like methyltransferase similar to S. cerevisiae SPB1 (YCL054W) putative rRNA methyltransferase
Caenorhabditis elegans CELE_H06I04.3   Protein H06I04.3, isoform B
Cryptosporidium hominis Chro.40181   FtsJ cell division protein
Cryptosporidium parvum cgd4_1580   Spb1p-like, FtsJ methylase
Dictyostelium discoideum DDB_G0284945   rRNA methyltransferase
Drosophila melanogaster Dmel_CG8939   CG8939 gene product from transcript CG8939-RA
Drosophila melanogaster Dmel_CG11447   CG11447 gene product from transcript CG11447-RA
Escherichia coli b3179   23S rRNA U2552 2'-O-ribose methyltransferase, SAM-dependent
Echinococcus granulosus EgrG_000874050   putative ribosomal RNA methyltransferase/cell division FtsJ protein
Echinococcus granulosus EgrG_000251100   rrna methyltransferase 3
Entamoeba histolytica EHI_088070   ribosomal RNA methyltransferase, putative
Echinococcus multilocularis EmuJ_000874050   ribosomal RNA large subunit methyltransferase
Echinococcus multilocularis EmuJ_000251100   rrna methyltransferase 3
Giardia lamblia GL50803_16993   FtsJ cell division protein, putative
Homo sapiens ENSG00000122687   FtsJ RNA methyltransferase homolog 2 (E. coli)
Homo sapiens ENSG00000108592   FtsJ homolog 3 (E. coli)
Leishmania braziliensis LbrM.27.2160   FtsJ cell division protein, putative
Leishmania donovani LdBPK_271900.1   FtsJ cell division protein, putative
Leishmania infantum LinJ.27.1900   FtsJ cell division protein, putative
Leishmania mexicana LmxM.27.1980   FtsJ cell division protein, putative
Loa Loa (eye worm) LOAG_07721   hypothetical protein
Loa Loa (eye worm) LOAG_14905   hypothetical protein
Loa Loa (eye worm) LOAG_05438   ribosomal RNA methyltransferase
Mus musculus ENSMUSG00000020706   FtsJ homolog 3 (E. coli)
Mus musculus ENSMUSG00000029557   FtsJ homolog 2 (E. coli)
Neospora caninum NCLIV_004720   ribosomal RNA large subunit methyltransferase J, putative
Neospora caninum NCLIV_002910   ftsJ-like methyltransferase domain-containing protein, putative
Oryza sativa 4339641   Os05g0567400
Onchocerca volvulus OVOC9451   Putative ribosomal RNA methyltransferase
Plasmodium berghei PBANKA_1130900   large subunit rRNA methyltransferase, putative
Plasmodium berghei PBANKA_1408100   ribosomal RNA methyltransferase, putative
Plasmodium falciparum PF3D7_1354300   large subunit rRNA methyltransferase, putative
Plasmodium falciparum PF3D7_1309600   ribosomal RNA methyltransferase, putative
Plasmodium knowlesi PKNH_1410000   ribosomal RNA methyltransferase, putative
Plasmodium knowlesi PKNH_1117500   large subunit rRNA methyltransferase, putative
Plasmodium vivax PVX_122310   ribosomal RNA large subunit methyltransferase J, putative
Plasmodium vivax PVX_114700   FtsJ-like methyltransferase, putative
Plasmodium yoelii PY02347   FtsJ cell division protein, putative
Plasmodium yoelii PY05898   FtsJ cell division protein
Plasmodium yoelii PY03106   FtsJ cell division protein, putative
Saccharomyces cerevisiae YCL054W   Spb1p
Saccharomyces cerevisiae YGL136C   Mrm2p
Schistosoma japonicum Sjp_0064940   ko:K00599 rRNA methyltransferase Spb1 [EC:2.1.1.-], putative
Schistosoma japonicum Sjp_0035560   ko:K02427 cell division protein methyltransferase FtsJ, putative
Schistosoma japonicum Sjp_0219450   expressed protein
Schistosoma japonicum Sjp_0064910   Putative rRNA methyltransferase 3, putative
Schistosoma mansoni Smp_045040   ribosomal RNA methyltransferase
Schistosoma mansoni Smp_148920   cell division protein ftsj
Schmidtea mediterranea mk4.001938.05   pre-rRNA processing protein FTSJ3
Schmidtea mediterranea mk4.004956.00   pre-rRNA processing protein FTSJ3
Schmidtea mediterranea mk4.002622.05   Putative ribosomal RNA methyltransferase
Schmidtea mediterranea mk4.001938.04   pre-rRNA processing protein FTSJ3
Trypanosoma brucei gambiense Tbg.972.2.2600   FtsJ cell division protein, putative
Trypanosoma brucei Tb927.2.4550   2'-O-ribose RNA methyltransferase SPB1 homolog
Trypanosoma congolense TcIL3000_0_06130   FtsJ cell division protein, putative
Trypanosoma congolense TcIL3000_2_900   FtsJ cell division protein, putative
Toxoplasma gondii TGME49_207990   ribosomal RNA large subunit methyltransferase J protein
Toxoplasma gondii TGME49_220940   ribosomal RNA methyltransferase (FtsJ) family protein
Treponema pallidum TP0682   cell division protein (ftsJ)
Theileria parva TP04_0351   ribosomal RNA methyltransferase, putative
Theileria parva TP04_0831   rRNA methyltransferase, putative
Trichomonas vaginalis TVAG_309050   ribosomal RNA methyltransferase, putative

Essentiality

Bm1_06985 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.2.4550 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.2.4550 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.2.4550 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.2.4550 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
b3179 Escherichia coli essential goodall
CELE_H06I04.3 Caenorhabditis elegans embryonic lethal wormbase
CELE_H06I04.3 Caenorhabditis elegans larval arrest wormbase
CELE_H06I04.3 Caenorhabditis elegans larval lethal wormbase
CELE_H06I04.3 Caenorhabditis elegans slow growth wormbase
CELE_H06I04.3 Caenorhabditis elegans sterile wormbase
YCL054W Saccharomyces cerevisiae inviable yeastgenome
PBANKA_1130900 Plasmodium berghei Essential plasmo
PBANKA_1408100 Plasmodium berghei Essential plasmo
TGME49_207990 Toxoplasma gondii Probably essential sidik
TGME49_220940 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Dengue virus 2 Genome polyprotein Compounds References
Dengue virus type 2 (strain Thailand/16681/1984) (DENV-2) Dengue virus type 2 NS3 protein Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0015 0.293 0.293
0.002 0.727 0.727

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier Bm1_06985 (Brugia malayi), rRNA methyltransferase
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