Detailed view for LmjF.14.1070

Basic information

TDR Targets ID: 25514
Leishmania major, protein kinase, putative
Source Database / ID:  TriTrypDB  GeneDB

pI: 5.1044 | Length (AA): 908 | MW (Da): 100434 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG2

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00069   Protein kinase domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0005524   ATP binding  
GO:0004713   protein tyrosine kinase activity  
GO:0004674   protein serine/threonine kinase activity  
GO:0004672   protein kinase activity  
GO:0006468   protein amino acid phosphorylation  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 9 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
365 424 1shf (A) 84 140 12.00 0.0000000014 0.03 0.09 -0.08
369 820 1qcf (A) 87 529 18.00 0 0.71 0.59 0.48
528 906 2eu9 (A) 136 475 24.00 0 1 0.43 0.07
573 807 2eu9 (A) 179 423 33.00 0 1 0.67 -1.08
383 823 1fmk (A) 127 529 19.00 0 0.95 0.450783 1.28
526 906 5ci6 (B) 38 351 25.00 0 1 0.361704 0.76
577 904 5lxc (A) 175 463 36.00 0 1 0.635134 -0.09
660 752 4yzn (A) 1131 1230 31.00 0.13 0.65 0.393323 -0.41
667 783 3f61 (A) 124 242 42.00 0.000000048 0.45 0.300755 0.88

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile metacyclic. Fernandes MC
Upregulation Percent Ranking Stage Dataset
Lower 20-40% percentile amastigotes. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_130162)

Species Accession Gene Product
Arabidopsis thaliana AT3G17750   protein kinase family protein
Arabidopsis thaliana AT1G73460   putative serine/threonine kinase
Arabidopsis thaliana AT1G73450   putative protein kinase
Arabidopsis thaliana AT2G40120   putative serine/threonine protein kinase
Babesia bovis BBOV_III009650   protein kinase domain containing protein
Cryptosporidium hominis Chro.70343   dual-specificity tyrosine-(Y)-phosphorylation regulated kinase TbPK4
Cryptosporidium parvum cgd7_3050   protein kinase
Giardia lamblia GL50803_17558   Kinase, CMGC DYRK
Giardia lamblia GL50803_137695   Kinase, CMGC DYRK
Leishmania braziliensis LbrM.14.1060   protein kinase, putative
Leishmania braziliensis LbrM.21.1940   protein kinase, putative
Leishmania donovani LdBPK_212010.1   protein kinase, putative
Leishmania donovani LdBPK_141140.1   protein kinase, putative
Leishmania infantum LinJ.14.1140   protein kinase, putative
Leishmania infantum LinJ.21.2010   protein kinase, putative
Leishmania major LmjF.21.1650   protein kinase, putative
Leishmania major LmjF.14.1070   protein kinase, putative
Leishmania mexicana LmxM.14.1070   protein kinase, putative
Leishmania mexicana LmxM.21.1650   protein kinase, putative
Neospora caninum NCLIV_020950   CMGC kinase, Dyrk family, putative
Oryza sativa 4327402   Os01g0832900
Oryza sativa 4333928   Os03g0719500
Oryza sativa 4339052   Os05g0466900
Trypanosoma brucei gambiense Tbg972.10.280   protein kinase, putative
Trypanosoma brucei gambiense Tbg972.7.4330   protein kinase, putative
Trypanosoma brucei Tb927.10.350   protein kinase PK4, putative
Trypanosoma brucei Tb927.7.3880   Basal body protein
Trypanosoma congolense TcIL3000_10_210   protein kinase, putative
Trypanosoma cruzi TcCLB.510519.40   protein kinase PK4, putative
Trypanosoma cruzi TcCLB.506869.60   protein kinase PK4, putative
Trypanosoma cruzi TcCLB.511249.60   CMGC/DYRK protein kinase, putative
Toxoplasma gondii TGME49_204280   cell-cycle-associated protein kinase DYRK, putative
Theileria parva TP04_0880   protein kinase, putative
Trichomonas vaginalis TVAG_000200   CMGC family protein kinase
Trichomonas vaginalis TVAG_396210   CMGC family protein kinase
Trichomonas vaginalis TVAG_048130   CMGC family protein kinase
Trichomonas vaginalis TVAG_238860   CMGC family protein kinase
Trichomonas vaginalis TVAG_362360   CMGC family protein kinase

Essentiality

LmjF.14.1070 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.7.3880 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.7.3880 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.7.3880 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.7.3880 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
Tb927.10.350 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.10.350 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.10.350 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.10.350 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_204280 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.4

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0039 0.5 0.5
0.0032 0.5 0.5
0.0037 1 0.5
0.0012 0.5 0.5
0.0012 0.5 0.5
0.0003 0.5 0.5
0.0012 0.5 0.5
0.0033 1 1
0.0016 0.5 0.5
0.0007 0.5 0.5
0.0063 0.7244 0.2543
0.0022 0.5 0.5
0.0059 1 1
0.0064 0.3377 0
0.0081 1 0.5
0.0088 0.4477 1
0.0056 1 0.5
0.0093 0.8828 0
0.0032 0.5 0.5
0.0016 0.5 0.5
0.0067 0.5 0.5
0.0027 1 0.5
0.0066 0.3101 0
0.0026 0.5 0.5
0.0039 0.5 0.5
0.0061 0.6883 0.5304
0.0036 0.5 0.5
0.0039 0.9485 0.5
0.0098 0.3242 0.2614
0.0007 0.5 0.5
0.0023 0.5 0.5
0.0029 0.5 0.5
0.0092 1 0.5
0.0011 1 0.5
0.0008 0.5 0.5
0.0062 0.6935 0
0.0059 1 1
0.0033 0.5 0.5
0.0063 1 1
0.0081 0.5 0.5
0.0091 1 0.5
0.0004 0.5 0.5
0.0007 0.5 0.5
0.0018 0.5 0.5
0.0042 0.5 0.5
0.0069 0.3067 1
0.0059 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier LmjF.14.1070 (Leishmania major), protein kinase, putative
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