Detailed view for TGME49_313930

Basic information

TDR Targets ID: 257459
Toxoplasma gondii, transmembrane protein
Source Database / ID:  ToxoDB 

pI: 7.6255 | Length (AA): 881 | MW (Da): 94913 | Paralog Number: 0

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 9

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF08627   CRT-like, chloroquine-resistance transporter-like

Gene Ontology

Mouse over links to read term descriptions.
No GO information for this protein.

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 2 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
40 174 2l7n (A) 1073 1202 13.00 0 0.02 0.274935 -0.67
103 173 2kr0 (A) 201 272 34.00 0.042 0.04 0.31739 0.91

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Sibley/Greg ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB

Orthologs

Ortholog group members (OG5_133154)

Species Accession Gene Product
Arabidopsis thaliana AT4G24460   CRT (chloroquine-resistance transporter)-like transporter 2
Arabidopsis thaliana AT5G12170   CRT (chloroquine-resistance transporter)-like transporter 3
Arabidopsis thaliana AT5G19380   CRT (chloroquine-resistance transporter)-like transporter 1
Babesia bovis BBOV_III003790   conserved hypothetical protein
Cryptosporidium hominis Chro.60564   transporter
Cryptosporidium parvum cgd6_4890   transporter protein with conserved Zn ribbon C11C7CxxC
Dictyostelium discoideum DDB_G0277321   chloroquine resistance transporter-like protein
Dictyostelium discoideum DDB_G0270204   chloroquine resistance transporter-like protein
Dictyostelium discoideum DDB_G0276943   chloroquine resistance transporter-like protein
Neospora caninum NCLIV_057130   hypothetical protein, conserved
Oryza sativa 4325409   Os01g0955700
Oryza sativa 4352325   Os12g0511300
Plasmodium berghei PBANKA_1219500   chloroquine resistance transporter, putative
Plasmodium falciparum PF3D7_0709000   chloroquine resistance transporter
Plasmodium knowlesi PKNH_0107600   chloroquine resistance transporter, putative
Plasmodium vivax PVX_087980   chloroquine resistance transporter, putative
Plasmodium yoelii PY05061   putative transporter protein CG10
Toxoplasma gondii TGME49_313930   transmembrane protein
Theileria parva TP02_0224   hypothetical protein

Essentiality

TGME49_313930 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1219500 Plasmodium berghei Essential plasmo
TGME49_313930 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Plasmodium falciparum chloroquine resistance transporter Compounds References
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0177 0.3356 1
0.0267 0.3073 1
0.0277 0.7725 1
0.0264 1 1
0.0157 0.7967 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier TGME49_313930 (Toxoplasma gondii), transmembrane protein
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