Detailed view for TGME49_241830

Basic information

TDR Targets ID: 257480
Toxoplasma gondii, peptidyl-prolyl cis-trans isomerase
Source Database / ID:  ToxoDB 

pI: 7.2052 | Length (AA): 575 | MW (Da): 64463 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00160   Cyclophilin type peptidyl-prolyl cis-trans isomerase/CLD

Gene Ontology

Mouse over links to read term descriptions.
GO:0000413   protein peptidyl-prolyl isomerization  
GO:0003755   peptidyl-prolyl cis-trans isomerase activity  
GO:0006457   protein folding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 8 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
7 188 2hq6 (A) 7 172 58.00 0 1 1.01 -1.17
9 207 1zkc (A) 276 450 44.00 0 1 0.81 -0.63
15 182 2fu0 (A) 8 158 35.00 0 1 0.75 -1.31
7 68 2hq6 (A) 7 68 71.00 0.0016 1 0.851626 0.2
8 197 3bkp (A) 17 206 99.00 0 1 1.47713 -1.74
449 574 5j0l (A) 3 129 14.00 0.96 0.01 0.41693 -1.03
458 566 2fxo (A) 841 949 14.00 0.35 0.01 0.336365 -0.52
463 554 4ktd (L) 51 130 34.00 0.77 0.01 0.0595 1.76

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

PDB tag
  • 3BKP:
  • Resolution Method # Atoms # Residues Dep. Date Pub. Date Mod. Date

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_129157)

Species Accession Gene Product
Arabidopsis thaliana AT4G33060   cyclophilin-like peptidyl-prolyl cis-trans isomerase family protein
Babesia bovis BBOV_II006340   peptidyl-prolyl cis-trans isomerase, cyclophilin-type family protein
Brugia malayi Bm1_29125   cyclophilin-type peptidyl-prolyl cis-trans isomerase-16, Bmcyp-16
Caenorhabditis elegans CELE_Y17G7B.9   Protein CYN-16
Dictyostelium discoideum DDB_G0268358   cyclophilin-type peptidylprolyl cis-trans isomerase
Drosophila melanogaster Dmel_CG10907   CG10907 gene product from transcript CG10907-RA
Echinococcus granulosus EgrG_000684950   peptidyl prolyl cis trans isomerase CWC27
Echinococcus multilocularis EmuJ_000684950   peptidyl prolyl cis trans isomerase CWC27
Homo sapiens ENSG00000153015   CWC27 spliceosome-associated protein homolog (S. cerevisiae)
Loa Loa (eye worm) LOAG_11869   hypothetical protein
Loa Loa (eye worm) LOAG_09606   cyclophilin-type peptidyl-prolyl cis-trans isomerase-16
Mus musculus ENSMUSG00000021715   CWC27 spliceosome-associated protein homolog (S. cerevisiae)
Neospora caninum NCLIV_017250   Peptidylprolyl isomerase (Cyclophilin)-like 2, related
Oryza sativa 4324199   Os01g0582400
Plasmodium berghei PBANKA_1020200   peptidyl-prolyl cis-trans isomerase, putative
Plasmodium falciparum PF3D7_1423200   peptidyl-prolyl cis-trans isomerase
Plasmodium knowlesi PKNH_1334800   peptidyl-prolyl cis-trans isomerase, putative
Plasmodium vivax PVX_085320   cyclophilin, putative
Plasmodium yoelii PY00017   peptidyl-prolyl cis-trans isomerase, cyclophilin-type, putative
Saccharomyces cerevisiae YPL064C   putative peptidylprolyl isomerase CWC27
Schistosoma japonicum Sjp_0084620   ko:K01802 peptidylprolyl isomerase [EC5.2.1.8], putative
Schistosoma mansoni Smp_084080   peptidyl-prolyl cis-trans isomerase (cyclophilin)
Schmidtea mediterranea mk4.014986.00   Peptidyl-prolyl cis-trans isomerase CWC27 homolog
Schmidtea mediterranea mk4.004419.06   Peptidyl-prolyl cis-trans isomerase CWC27 homolog
Toxoplasma gondii TGME49_241830   peptidyl-prolyl cis-trans isomerase
Theileria parva TP02_0598   cyclophilin, putative

Essentiality

TGME49_241830 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1020200 Plasmodium berghei Dispensable plasmo
TGME49_241830 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Plasmodium falciparum peptidyl-prolyl cis-trans isomerase 171 aa 33.5% 155 aa Compounds References
Rattus norvegicus Cyclophilin A 164 aa 30.6% 157 aa Compounds References
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier TGME49_241830 (Toxoplasma gondii), peptidyl-prolyl cis-trans isomerase
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