Detailed view for LmjF.34.1330

Basic information

TDR Targets ID: 25840
Leishmania major, hypothetical protein, conserved
Source Database / ID:  TriTrypDB  GeneDB

pI: 8.0673 | Length (AA): 406 | MW (Da): 46041 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00004   ATPase family associated with various cellular activities (AAA)
PF08740   BCS1 N terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0034551   GO:mitochondrial respiratory chain complex III assembly  

GO:0017111   nucleoside-triphosphatase activity  
GO:0005524   ATP binding  
GO:0000166   nucleotide binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 404 1e32 (A) 41 453 18.00 0 1 0.92 0.38
170 321 1ixz (A) 168 336 33.00 0 1 0.89 -1.46
144 215 1sxj (C) 15 70 39.00 0.37 0.48 0.26404 0.66
149 399 2ce7 (C) 151 438 25.00 0 1 0.800427 0.51
172 313 4ww0 (A) 169 328 31.00 0.0000000021 1 0.811454 -1.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile amastigotes, metacyclic. Fernandes MC
Show/Hide expression data references
  • Fernandes MC Dual Transcriptome Profiling of Leishmania-Infected Human Macrophages Reveals Distinct Reprogramming Signatures.

Orthologs

Ortholog group members (OG5_128574)

Species Accession Gene Product
Arabidopsis thaliana AT4G05380   AAA-type ATPase family protein
Brugia malayi Bm1_30135   mitochondrial chaperone BCS1
Candida albicans CaO19.458   Mitochondrial ATPase (AAA type).
Candida albicans CaO19.8089   Mitochondrial ATPase (AAA type).
Caenorhabditis elegans CELE_F54C9.6   Protein BCS-1, isoform B
Dictyostelium discoideum DDB_G0291910   mitochondrial ATPase
Dictyostelium discoideum DDB_G0289135   mitochondrial ATPase
Drosophila melanogaster Dmel_CG4908   CG4908 gene product from transcript CG4908-RB
Echinococcus granulosus EgrG_001078300   mitochondrial chaperone BCS1
Echinococcus granulosus EgrG_001078400   mitochondrial chaperone BCS1
Echinococcus multilocularis EmuJ_001078400   mitochondrial chaperone BCS1
Echinococcus multilocularis EmuJ_001078300   mitochondrial chaperone BCS1
Homo sapiens ENSG00000074582   BC1 (ubiquinol-cytochrome c reductase) synthesis-like
Leishmania braziliensis LbrM.20.1360   hypothetical protein, conserved
Leishmania donovani LdBPK_341440.1   BCS1 N terminal/ATPase family associated with various cellular activities (AAA), putative
Leishmania infantum LinJ.34.1440   hypothetical protein, conserved
Leishmania major LmjF.34.1330   hypothetical protein, conserved
Leishmania mexicana LmxM.33.1330   hypothetical protein, conserved
Loa Loa (eye worm) LOAG_07266   chaperone BCS1
Mus musculus ENSMUSG00000026172   BCS1-like (yeast)
Neospora caninum NCLIV_018220   Mitochondrial protein-like, related
Oryza sativa 4333339   Os03g0584400
Oryza sativa 4326559   Os01g0641800
Oryza sativa 4343389   Os07g0517600
Plasmodium berghei PBANKA_0102000   mitochondrial chaperone BCS1, putative
Plasmodium falciparum PF3D7_0603200   mitochondrial chaperone BCS1, putative
Plasmodium knowlesi PKNH_1147500   mitochondrial chaperone BCS1, putative
Plasmodium vivax PVX_113325   mitochondrial chaperone BCS1, putative
Plasmodium yoelii PY02578   bcs1 protein
Saccharomyces cerevisiae YDR375C   bifunctional AAA family ATPase chaperone/translocase BCS1
Schistosoma japonicum Sjp_0062800   ko:K08900 mitochondrial chaperone BCS1, putative
Schistosoma mansoni Smp_083120   mitochondrial chaperone BCS1
Schmidtea mediterranea mk4.000282.07   Mitochondrial chaperone BCS1
Trypanosoma brucei gambiense Tbg972.8.3290   ATP-dependent chaperone, putative,mitochondrial chaperone BCS1, putative
Trypanosoma brucei Tb927.8.3580   mitochondrial chaperone BCS1, putative
Trypanosoma congolense TcIL3000_8_3540   ATP-dependent chaperone, putative
Trypanosoma cruzi TcCLB.509553.20   ATP-dependent chaperone, putative
Trypanosoma cruzi TcCLB.505945.50   ATP-dependent chaperone, putative
Toxoplasma gondii TGME49_243490   BCS1 family isoform 9, putative

Essentiality

LmjF.34.1330 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.8.3580 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb927.8.3580 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb927.8.3580 Trypanosoma brucei no significant loss or gain of fitness in procyclic forms alsford
Tb927.8.3580 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F54C9.6 Caenorhabditis elegans embryonic lethal wormbase
CELE_F54C9.6 Caenorhabditis elegans larval arrest wormbase
CELE_F54C9.6 Caenorhabditis elegans slow growth wormbase
PBANKA_0102000 Plasmodium berghei Essential plasmo
TGME49_243490 Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

User comments

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Gene identifier LmjF.34.1330 (Leishmania major), hypothetical protein, conserved
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