Detailed view for TGME49_226560

Basic information

TDR Targets ID: 258927
Toxoplasma gondii, zinc finger (CCCH type) motif-containing protein
Source Database / ID:  ToxoDB 

pI: 5.8628 | Length (AA): 401 | MW (Da): 45182 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00642   Zinc finger C-x8-C-x5-C-x3-H type (and similar)
PF14634   zinc-RING finger domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0046872   metal ion binding  
GO:0008270   zinc ion binding  
GO:0005515   protein binding  
GO:0003676   nucleic acid binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 3 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
216 312 1joc (A) 1308 1411 21.00 0 0.92 0.504895 -0.5
249 326 2csy (A) 2 75 50.00 0 1 0.756514 -0.33
342 401 5eiu (A) 96 157 7.00 0.37 0 0.425426 -2.49

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile ME49 Tachyzoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Fritz HM Sibley/Greg
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile VEG Tachyzoite, ME49 merozoite. Gregory Hehl AB
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_128440)

Species Accession Gene Product
Arabidopsis thaliana AT1G01350   zinc finger CCCH domain-containing protein 1
Arabidopsis thaliana AT5G06420   zinc finger CCCH domain-containing protein 51
Babesia bovis BBOV_IV009570   zinc finger protein, putative
Brugia malayi Bm1_06930   Zinc finger protein 183 homolog
Candida albicans CaO19.9653   part of spliceosome complex
Candida albicans CaO19.2105   has Zn finger similar to that of spliceosome associated protein
Caenorhabditis elegans CELE_K01G5.1   Protein RNF-113
Cryptosporidium hominis Chro.60383   zf-C3HC4/zf-CCCH zinc finger protein
Cryptosporidium parvum cgd6_3300   Yir323cp/Cwc24 p family; CCCH+ringfinger domains
Dictyostelium discoideum DDB_G0267870   CCCH-type zinc finger-containing protein
Drosophila melanogaster Dmel_CG4973   midlife crisis
Echinococcus granulosus EgrG_000255700   ring finger protein 113a
Entamoeba histolytica EHI_192080   zinc finger protein, putative
Echinococcus multilocularis EmuJ_000255700   ring finger protein 113a
Homo sapiens ENSG00000125352   ring finger protein 113A
Homo sapiens ENSG00000139797   ring finger protein 113B
Loa Loa (eye worm) LOAG_01140   RiNg Finger protein family member
Mus musculus ENSMUSG00000036537   ring finger protein 113A1
Mus musculus ENSMUSG00000098134   ring finger protein 113A2
Neospora caninum NCLIV_046420   Pre-mRNA-splicing factor CWC24, related
Oryza sativa 4329092   Os02g0301000
Plasmodium berghei PBANKA_1307700   pre-mRNA-splicing factor CWC24, putative
Plasmodium falciparum PF3D7_1443800   pre-mRNA-splicing factor CWC24, putative
Plasmodium knowlesi PKNH_1238500   pre-mRNA-splicing factor CWC24, putative
Plasmodium vivax PVX_118300   hypothetical protein, conserved
Plasmodium yoelii PY01907   Arabidopsis thaliana MHF15.6
Saccharomyces cerevisiae YLR323C   Cwc24p
Schistosoma japonicum Sjp_0207800   RING finger protein 113A, putative
Schistosoma japonicum Sjp_0043170   RING finger protein 113A, putative
Schistosoma mansoni Smp_033590   hypothetical protein
Schmidtea mediterranea mk4.016364.00   RING finger protein 113 homolog
Schmidtea mediterranea mk4.028451.00  
Schmidtea mediterranea mk4.003072.05   RING finger protein 113 homolog
Schmidtea mediterranea mk4.013967.00   RING finger protein 113 homolog
Schmidtea mediterranea mk4.012092.01   RING finger protein 113 homolog
Toxoplasma gondii TGME49_226560   zinc finger (CCCH type) motif-containing protein
Theileria parva TP01_0812   hypothetical protein, conserved
Trichomonas vaginalis TVAG_185490   cell cycle control protein cwf24, putative

Essentiality

TGME49_226560 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_K01G5.1 Caenorhabditis elegans embryonic lethal wormbase
CELE_K01G5.1 Caenorhabditis elegans larval arrest wormbase
CELE_K01G5.1 Caenorhabditis elegans sterile wormbase
YLR323C Saccharomyces cerevisiae inviable yeastgenome
TGME49_226560 this record Toxoplasma gondii Probably essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_226560 (Toxoplasma gondii), zinc finger (CCCH type) motif-containing protein
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