Detailed view for PF3D7_1428300

Basic information

TDR Targets ID: 2592
Plasmodium falciparum, proliferation-associated protein 2g4, putative
Source Database / ID:  PlasmoDB   |   GeneDB   |   MPMP

pI: 7.8419 | Length (AA): 377 | MW (Da): 42641 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG3

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00557   Metallopeptidase family M24

Gene Ontology

Mouse over links to read term descriptions.
GO:0005575   cellular_component  
GO:0009987   cellular process  
GO:0008283   cell proliferation  
GO:0007050   cell cycle arrest  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
1 334 1wy2 (A) 120 349 17.00 0 1 0.56 0.62
10 329 1b6a () 162 473 19.00 0 1 1.11 -0.74
12 325 1xgs (A) 1 290 27.00 0 1 1.14 -0.44
3 358 2q8k (A) 7 362 30.00 0 1 1.409 -0.98

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile intra-erythrocytic - 0 hs, intra-erythrocytic - 8 hs, intra-erythrocytic - 16 hs, intra-erythrocytic - 24 hs, intra-erythrocytic - 32 hs, merozoite, sporozoite, early ring, early schizont, early trophozoite, late ring, late trophozoite, Oocyst, Ring. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile intra-erythrocytic - 40 hs, intra-erythrocytic - 48 hs, gametocyte, late schizont, Sporozoite. Otto TD PlasmoDB Zanghi G
Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile Female Gametocyte, Male Gametocyte. Lasonder E
Show/Hide expression data references
  • Otto TD New insights into the blood-stage transcriptome of Plasmodium falciparum using RNA-Seq.
  • Lasonder E Integrated transcriptomic and proteomic analyses of P. falciparum gametocytes. Molecular insight into sex-specific processes and translational repression.
  • Zanghi G A Specific PfEMP1 Is Expressed in P. falciparum Sporozoites and Plays a Role in Hepatocyte Infection.
  • PlasmoDB Data on upregulation of P. falciparum genes in different life cycle stages, combined from several microarray experiments available in PlasmoDB

Orthologs

Ortholog group members (OG5_128136)

Species Accession Gene Product
Arabidopsis thaliana AT3G51800   putative nuclear DNA-binding protein G2p
Babesia bovis BBOV_IV005570   proliferation-associated protein 2g4, putative
Brugia malayi Bm1_12685   DNA-binding protein, 42 kDa containing protein
Candida albicans CaO19.6507   curved-DNA binding protein
Candida albicans CaO19_6507   hypothetical protein
Candida albicans CaO19.13860   curved-DNA binding protein
Caenorhabditis elegans CELE_W08E12.7   Protein W08E12.7
Cryptosporidium hominis Chro.30278   nuclear DNA-binding protein G2p -related
Cryptosporidium parvum cgd3_2390   proliferation-associated protein 2G4 metalloprotease, creatinase/aminopeptidase fold
Dictyostelium discoideum DDB_G0282361   proliferation associated protein
Drosophila melanogaster Dmel_CG10576   CG10576 gene product from transcript CG10576-RD
Echinococcus granulosus EgrG_000781200   Proliferation associated protein 2G4
Entamoeba histolytica EHI_175020   peptidase, putative
Echinococcus multilocularis EmuJ_000781200   Proliferation associated protein 2G4
Homo sapiens ENSG00000170515   proliferation-associated 2G4, 38kDa
Leishmania braziliensis LbrM.19.0470   aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Leishmania donovani LdBPK_190150.1   metallo-peptidase, Clan MG, Family M24
Leishmania infantum LinJ.19.0150   aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Leishmania major LmjF.19.0160   aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Leishmania mexicana LmxM.19.0160   aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Loa Loa (eye worm) LOAG_08033   DNA-binding protein
Mus musculus ENSMUSG00000025364   proliferation-associated 2G4
Neospora caninum NCLIV_038400   Methionine aminopeptidase (EC 3.4.11.18), related
Oryza sativa 4338500   Os05g0350500
Plasmodium berghei PBANKA_1016300   proliferation-associated protein 2g4, putative
Plasmodium falciparum PF3D7_1428300   proliferation-associated protein 2g4, putative
Plasmodium knowlesi PKNH_1330400   proliferation-associated protein, putative
Plasmodium vivax PVX_085125   proliferation-associated protein 2g4, putative
Schistosoma japonicum Sjp_0128810   ko:K01265 methionyl aminopeptidase [EC3.4.11.18], putative
Schistosoma mansoni Smp_150690.1   proliferation-associated protein 2G4 38kDa (M24 family)
Schistosoma mansoni Smp_150690.2   proliferation-associated protein 2G4 38kDa (M24 family)
Schmidtea mediterranea mk4.000573.03   Proliferation-associated protein 2G4, 38kDa
Trypanosoma brucei gambiense Tbg972.10.17950   aminopeptidase, putative,metallo-peptidase, Clan MG, Family M24
Trypanosoma brucei Tb927.10.14790   metallo-peptidase, Clan MG, Family M24
Trypanosoma congolense TcIL3000_10_12660   metallo-peptidase, Clan MG, Family M24
Trypanosoma cruzi TcCLB.506211.200   metallo-peptidase, Clan MG, Family M24
Trypanosoma cruzi TcCLB.511289.30   metallo-peptidase, Clan MG, Family M24
Toxoplasma gondii TGME49_279390   proliferation-associated protein 2G4, putative
Theileria parva TP01_0364   proliferation-associated protein 2g4, putative

Essentiality

PF3D7_1428300 has one or more orthologs with essentiality data
Gene/Ortholog Organism Phenotype Source Study
Tb927.10.14790 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.10.14790 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.10.14790 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.10.14790 Trypanosoma brucei significant loss of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_279390 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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User comments

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Gene identifier PF3D7_1428300 (Plasmodium falciparum), proliferation-associated protein 2g4, putative
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