Detailed view for TGME49_229480

Basic information

TDR Targets ID: 259271
Toxoplasma gondii, calcium binding protein precursor, putative
Source Database / ID:  ToxoDB 

pI: 4.3072 | Length (AA): 335 | MW (Da): 38291 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 1

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF13405   EF-hand domain
PF13499   EF-hand domain pair

Gene Ontology

Mouse over links to read term descriptions.
GO:0005509   calcium ion binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 4 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
76 296 4rgj (A) 302 525 28.00 0.00001 1 0.890902 0
141 215 1rwy (A) 19 102 40.00 0.37 1 0.729081 -1.34
152 217 2pmy (A) 7 69 22.00 0 0.91 0.585915 -1.66
152 287 4ds7 (A) 7 143 24.00 0 1 0.78387 -1

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_129808)

Species Accession Gene Product
Arabidopsis thaliana AT5G08580   EF-hand, calcium binding motif-containing protein
Arabidopsis thaliana AT4G27790   Calcium-binding EF hand family protein
Babesia bovis BBOV_III007520   membrane-associated calcum-binding protein, putative
Brugia malayi Bm1_55540   EF hand family protein
Caenorhabditis elegans CELE_M03F4.7   Protein CALU-1, isoform B
Cryptosporidium hominis Chro.20427   membrane-associated calcum-binding protein
Cryptosporidium parvum cgd2_3990   protein with signal peptide, ER retention motif and 4x EF hands
Drosophila melanogaster Dmel_CG9148   supercoiling factor
Echinococcus granulosus EgrG_000245300   calumenin
Echinococcus multilocularis EmuJ_000245300   calumenin
Homo sapiens 813   calumenin
Loa Loa (eye worm) LOAG_05293   EF hand family protein
Mus musculus ENSMUSG00000029767   calumenin
Neospora caninum NCLIV_030420   Rcn2-prov protein, related
Oryza sativa 4337432   Os04g0681500
Plasmodium berghei PBANKA_0938400   endoplasmic reticulum-resident calcium binding protein, putative
Plasmodium falciparum PF3D7_1108600   endoplasmic reticulum-resident calcium binding protein
Plasmodium knowlesi PKNH_0906200   endoplasmic reticulum-resident calcium binding protein, putative
Plasmodium vivax PVX_091105   endoplasmic reticulum-resident calcium binding protein, putative
Plasmodium yoelii PY05050   membrane-associated calcum-binding protein
Schistosoma japonicum Sjp_0032650   IPR002048,Calcium-binding EF-hand,domain-containing
Schistosoma japonicum Sjp_0025210   Calumenin precursor, putative
Schistosoma japonicum Sjp_0072720   Reticulocalbin-1 precursor, putative
Schistosoma mansoni Smp_103740.2   calmodulin related calcium binding protein
Schistosoma mansoni Smp_103740.1   calmodulin related calcium binding protein
Schistosoma mansoni Smp_005230.1   reticulocalbin
Schistosoma mansoni Smp_005230.3   reticulocalbin
Schistosoma mansoni Smp_005230.2   reticulocalbin
Schmidtea mediterranea mk4.016806.00   Reticulocalbin-2
Schmidtea mediterranea mk4.005455.00   Calumenin-like protein
Schmidtea mediterranea mk4.001404.06  
Schmidtea mediterranea mk4.002060.02   Calumenin-like protein
Schmidtea mediterranea mk4.001100.05  
Schmidtea mediterranea mk4.003282.02  
Schmidtea mediterranea mk4.001284.04  
Toxoplasma gondii TGME49_229480   calcium binding protein precursor, putative
Theileria parva TP04_0681   membrane-associated calcium-binding protein, putative

Essentiality

TGME49_229480 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
CELE_M03F4.7 Caenorhabditis elegans larval arrest wormbase
CELE_M03F4.7 Caenorhabditis elegans larval lethal wormbase
TGME49_229480 this record Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Bos taurus Calmodulin 149 aa 24.2% 132 aa Compounds References
Obtained from network model

Ranking Plot

No enough druggable targets predicted through repurposing network model to make a plot

Putative Drugs List

Compound Raw Global Species
0.0016 0.2966 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

If you have references for this gene, please enter them in a user comment (below) or Contact us.

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Gene identifier TGME49_229480 (Toxoplasma gondii), calcium binding protein precursor, putative
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