Detailed view for TGME49_224600

Basic information

TDR Targets ID: 259382
Toxoplasma gondii, GTP binding protein
Source Database / ID:  ToxoDB 

pI: 7.5048 | Length (AA): 451 | MW (Da): 50624 | Paralog Number: 0

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG1

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF01926   50S ribosome-binding GTPase
PF08438   GTPase of unknown function C-terminal

Gene Ontology

Mouse over links to read term descriptions.
GO:0005622   intracellular  
GO:0005525   GTP binding  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 6 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
4 447 1jal (A) 1 362 22.00 0 1 0.79 0.73
8 446 1wxq (A) 3 394 34.00 0 1 1.05 0.52
6 446 1wxq (A) 1 394 29.00 0.0000000024 1 0.966227 0.96
8 80 4dhe (B) 28 117 19.00 0 0.05 0.407463 -0.62
8 446 1wxq (A) 3 394 34.00 0 1 1.03509 0.72
8 46 2wji (A) 6 43 52.00 0.67 0.47 0.463174 0.04

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Mid 40-60% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Gregory ToxoDB
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_130225)

Species Accession Gene Product
Babesia bovis BBOV_IV001640   GTP binding protein, putative
Candida albicans CaO19.4846   similar to S. cerevisiae YGR210C
Candida albicans CaO19.12309   similar to S. cerevisiae YGR210C
Cryptosporidium hominis Chro.60204   GTP-binding protein
Cryptosporidium parvum cgd6_1670   MJ1332/Ygr210cp-like GTP binding protein; GTpase OBG family plus RNA binding domain TGS
Dictyostelium discoideum DDB_G0283773   hypothetical protein
Leishmania braziliensis LbrM.32.3400   hypothetical protein, conserved
Leishmania donovani LdBPK_323310.1   Serine hydrolase (FSH1)/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Leishmania infantum LinJ.32.3310   hypothetical protein, conserved
Leishmania major LmjF.32.3110   hypothetical protein, conserved
Leishmania mexicana LmxM.31.3110   hypothetical protein, conserved
Neospora caninum NCLIV_048120   GTP-binding protein, putative
Saccharomyces cerevisiae YGR210C   hypothetical protein
Trypanosoma brucei gambiense Tbg972.11.19070   hypothetical protein, conserved
Trypanosoma brucei Tb11.v5.0491   Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase, putative
Trypanosoma brucei Tb927.11.16910   Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Trypanosoma congolense TcIL3000_0_44730   Serine hydrolase (FSH1)/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Trypanosoma cruzi TcCLB.503749.10   Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Trypanosoma cruzi TcCLB.511021.99   Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Trypanosoma cruzi TcCLB.508709.10   Serine hydrolase (FSH1)/RWD domain/50S ribosome-binding GTPase/GTPase of unknown function C-terminal, putative
Toxoplasma gondii TGME49_224600   GTP binding protein

Essentiality

TGME49_224600 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb11.01.8680 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (3 days) alsford
Tb11.01.8680 Trypanosoma brucei no significant loss or gain of fitness in bloodstream forms (6 days) alsford
Tb11.01.8680 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb11.01.8680 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
TGME49_224600 this record Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model
No druggable targets predicted through repurposing network model

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_224600 (Toxoplasma gondii), GTP binding protein
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