Detailed view for TGME49_215170

Basic information

TDR Targets ID: 261328
Toxoplasma gondii, serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, putative
Source Database / ID:  ToxoDB 

pI: 5.3625 | Length (AA): 552 | MW (Da): 60376 | Paralog Number: 1

Signal peptide: N | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00149   Calcineurin-like phosphoesterase

Gene Ontology

Mouse over links to read term descriptions.
GO:0016787   hydrolase activity  

Metabolic Pathways

mRNA surveillance pathway (KEGG)

Structural information

Modbase 3D models:

There are 5 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
48 159 4cgw (A) 269 381 11.00 0.58 0.02 0.415199 -1.23
59 542 4ja7 (A) 26 486 25.00 0 1 1.12711 -0.03
216 538 1aui (A) 40 340 39.00 0 1 1.06304 -1.16
217 364 4iyp (C) 8 153 55.00 0 1 0.677016 -0.27
217 552 3fga (C) 8 309 57.00 0 1 1.2476 -1.07

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 80-100% percentile VEG Tachyzoite. Gregory
Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Tachyzoite, ME49 merozoite, ME49 Oocyst, ME49 Bradyzoite. Gregory Hehl AB Fritz HM Sibley/Greg
Show/Hide expression data references
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Gregory ToxoDB
  • Sibley/Greg ToxoDB

Orthologs

Ortholog group members (OG5_127141)

Species Accession Gene Product
Arabidopsis thaliana AT1G69960   serine/threonine protein phosphatase 2A
Arabidopsis thaliana AT1G10430   protein phosphatase 2A-2
Arabidopsis thaliana AT3G58500   serine/threonine-protein phosphatase PP2A-3 catalytic subunit
Arabidopsis thaliana AT2G42500   serine/threonine-protein phosphatase PP2A-3 catalytic subunit
Arabidopsis thaliana AT1G59830   serine/threonine-protein phosphatase PP2A-1 catalytic subunit
Babesia bovis BBOV_I000740   serine/threonine protein phosphatase PP2A catalytic subunit
Brugia malayi Bm1_38390   protein phosphatase 2A.
Brugia malayi Bm1_34045   type 2A protein phosphatase catalytic subunit
Brugia malayi Bm1_41290   Ser/Thr protein phosphatase family protein
Candida albicans CaO19.1683   Serine-Threonine protein phosphatase 2A.
Candida albicans CaO19.9252   Serine-Threonine protein phosphatase 2A.
Caenorhabditis elegans CELE_C05A2.1   Protein PPH-1
Caenorhabditis elegans CELE_F38H4.9   Protein LET-92
Cryptosporidium hominis Chro.70100   hypothetical protein
Cryptosporidium hominis Chro.50440   hypothetical protein
Cryptosporidium parvum cgd7_810   protein phosphatase PP2A, calcineurin like phosphoesterase superfamily
Cryptosporidium parvum cgd5_4070   hypothetical protein
Dictyostelium discoideum DDB_G0290263   protein phosphatase 2A catalytic subunit
Drosophila melanogaster Dmel_CG7109   microtubule star
Echinococcus granulosus EgrG_001106500   serine:threonine protein phosphatase 2A
Entamoeba histolytica EHI_011950   serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, putative
Echinococcus multilocularis EmuJ_001106500   serine:threonine protein phosphatase 2A
Giardia lamblia GL50803_5010   Ser/Thr phosphatase PP2A-2 catalytic subunit
Homo sapiens 5515   protein phosphatase 2, catalytic subunit, alpha isozyme
Homo sapiens ENSG00000104695   protein phosphatase 2, catalytic subunit, beta isozyme
Leishmania donovani LdBPK_251360.1   serine/threonine-protein phosphatase
Leishmania infantum LinJ.25.1360   serine/threonine protein phosphatase, putative
Leishmania major LmjF.25.1320   serine/threonine protein phosphatase, putative
Leishmania mexicana LmxM.25.1320   serine/threonine protein phosphatase, putative
Loa Loa (eye worm) LOAG_09990   hypothetical protein
Loa Loa (eye worm) LOAG_03638   type 2A protein phosphatase catalytic subunit
Loa Loa (eye worm) LOAG_08810   LEThal family member
Mus musculus ENSMUSG00000009630   protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform
Mus musculus 19052   protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform
Neospora caninum NCLIV_048330   hypothetical protein
Neospora caninum NCLIV_052160   Serine/threonine protein phosphatase (EC 3.1.3.16), related
Oryza sativa 4348601   Os10g0410600
Oryza sativa 4328734   Os02g0217600
Oryza sativa 4341364   Os06g0574500
Oryza sativa 4334494   Os03g0805300
Oryza sativa 4331747   Os03g0167700
Plasmodium berghei PBANKA_0826200   protein phosphatase-beta, putative
Plasmodium falciparum PF3D7_0925400   protein phosphatase-beta
Plasmodium knowlesi PKNH_0723300   protein phosphatase-beta, putative
Plasmodium vivax PVX_099685   protein phosphatase-beta, putative
Plasmodium yoelii PY00926   protein phosphatase-beta
Saccharomyces cerevisiae YDL188C   phosphoprotein phosphatase 2A catalytic subunit PPH22
Saccharomyces cerevisiae YDL134C   phosphoprotein phosphatase 2A catalytic subunit PPH21
Schistosoma japonicum Sjp_0083710   Serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, putative
Schistosoma mansoni Smp_165490   protein phsophatase-2a
Schmidtea mediterranea mk4.000058.24   Serine/threonine-protein phosphatase PP2A
Schmidtea mediterranea mk4.000033.06   Serine/threonine-protein phosphatase 6 catalytic subunit
Schmidtea mediterranea mk4.015572.00   Serine/threonine-protein phosphatase PP2A
Schmidtea mediterranea mk4.001170.02   Serine/threonine-protein phosphatase PP2A
Schmidtea mediterranea mk4.000206.02   FI07629p
Schmidtea mediterranea mk4.025254.02  
Schmidtea mediterranea mk4.002904.03   Serine/threonine-protein phosphatase PP2A
Schmidtea mediterranea mk4.032791.00  
Trypanosoma brucei gambiense Tbg972.3.990   serine/threonine-protein phosphatase, putative
Trypanosoma brucei Tb927.3.1240   Serine/threonine-protein phosphatase 2A, putative
Trypanosoma congolense TcIL3000_3_490   serine/threonine-protein phosphatase
Trypanosoma cruzi TcCLB.511881.20   serine/threonine-protein phosphatase
Trypanosoma cruzi TcCLB.509453.50   serine/threonine-protein phosphatase
Toxoplasma gondii TGME49_224220   serine/threonine-protein phosphatase PP2A catalytic subunit
Toxoplasma gondii TGME49_215170   serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, putative
Theileria parva TP02_0853   serine/threonine protein phosphatase, putative
Trichomonas vaginalis TVAG_243240   protein phosphatase-1, putative

Essentiality

TGME49_215170 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
Tb927.3.1240 Trypanosoma brucei significant loss of fitness in bloodstream forms (3 days) alsford
Tb927.3.1240 Trypanosoma brucei significant loss of fitness in bloodstream forms (6 days) alsford
Tb927.3.1240 Trypanosoma brucei significant loss of fitness in procyclic forms alsford
Tb927.3.1240 Trypanosoma brucei no significant loss or gain of fitness in differentiation of procyclic to bloodstream forms alsford
CELE_F38H4.9 Caenorhabditis elegans embryonic lethal wormbase
CELE_F38H4.9 Caenorhabditis elegans larval arrest wormbase
CELE_F38H4.9 Caenorhabditis elegans larval lethal wormbase
CELE_F38H4.9 Caenorhabditis elegans sterile wormbase
PBANKA_0826200 Plasmodium berghei Essential plasmo
TGME49_215170 this record Toxoplasma gondii Probably essential sidik
TGME49_224220 Toxoplasma gondii Probably essential sidik
TGME49_215170 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_224220 Toxoplasma gondii Essentiality uncertain sidik
Show/Hide essentiality data references
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Druggability index (range: 0 to 1): 0.3

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Species Known druggable target Linked compounds Reference
Bos taurus Serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform Compounds References
Homo sapiens protein phosphatase 2, catalytic subunit, alpha isozyme Compounds References
By sequence similarity to non orthologous druggable targets
Species Target Length Identity Alignment span Linked Drugs Reference
Oryctolagus cuniculus Serine/threonine protein phosphatase PP1-alpha catalytic subunit 330 aa 40.3% 298 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative 351 aa 37.1% 315 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative 294 aa 35.1% 299 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1 beta 330 aa 33.9% 330 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1 alpha 169 aa 29.9% 197 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative 294 aa 34.1% 320 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative 159 aa 27.8% 180 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1 alpha 297 aa 34.5% 322 aa Compounds References
Trypanosoma cruzi serine/threonine-protein phosphatase PP1, putative 294 aa 35.1% 299 aa Compounds References
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.0082 0.3989 1
0.0029 1 1
0.0029 1 1
0.0029 1 1
0.0053 0.2588 0.72
0.0029 1 1
0.0029 1 1
0.0054 0.2586 1
0.0023 1 1

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_215170 (Toxoplasma gondii), serine/threonine-protein phosphatase 2A catalytic subunit beta isoform, putative
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