Detailed view for TGME49_223700

Basic information

TDR Targets ID: 261523
Toxoplasma gondii, F5/8 type C domain-containing protein
Source Database / ID:  ToxoDB 

pI: 4.976 | Length (AA): 1571 | MW (Da): 170535 | Paralog Number: 2

Signal peptide: Y | GPI Anchor: N | Predicted trans-membrane segments: 0

Druggability Group : DG5

Targets have been classified into druggability groups (DG) according to their druggability score in network driven prioritizations. DGs range from 1 to 5; the higher the group number, the higher the chance of the target to be druggable

Pfam domains

PF00652   Ricin-type beta-trefoil lectin domain
PF00754   F5/8 type C domain
PF03815   LCCL domain

Gene Ontology

Mouse over links to read term descriptions.
GO:0062023   GO:collagen-containing extracellular matrix  

GO:0007155   cell adhesion  

Metabolic Pathways

This gene is not mapped to any metabolic pathway in KEGG.

Structural information

Modbase 3D models:

There are 10 models calculated for this protein. More info on these models, including the models themselves is available at: Modbase

Target Beg Target End Template Template Beg Template End Identity Evalue Model Score MPQS zDope
263 403 1czt (A) 9 157 14.00 0 0.58 -0.06 -0.5
264 403 1tvg (A) 5 138 18.00 0 0.97 0.14 -0.5
687 788 1jbi (A) 3 97 36.00 0 1 0.32 -0.18
26 149 4zy9 (A) 1 126 15.00 0.000014 0.32 0.123831 -0.05
32 131 2j1a (A) 630 738 17.00 0.92 0.12 0.0235537 0.5
296 431 1tvg (A) 10 138 16.00 0 0.99 0.184869 -0.01
298 373 4zz5 (A) 6 78 34.00 0.2 0.4 0.326177 0.33
299 428 4zy9 (A) 6 129 25.00 0 0.96 0.35505 -0.58
739 840 1jbi (A) 1 94 38.00 0 1 0.427227 -0.04
1044 1190 4b1m (A) 535 677 15.00 0 0.97 0.338871 -0.96

Help me make sense of these data.

Target Beg: first modeled residue
Target End: last modeled residue
Template: template structure used for modelling (PDB accession and chain)
Template Beg: first template residue in target-template alignment
Template End: last template residue in target-template alignment
Identity: sequence identity
Evalue: E value for target-template hit
Model Score: GA341 score (>0.7 for reliable model)
MPQS: ModPipe Quality Score (>1.1 for reliable model)
zDope: zDope Score (negative for reliable model)

A more detailed description of these scores is available at the Modbase Model Evaluation Help Pages, and in the papers referenced therein.

PDB Structures:

No structure availble in the PDB for this protein

Expression

Upregulation Percent Ranking Stage Dataset
Upper 60-80% percentile ME49 Oocyst. Fritz HM
Upregulation Percent Ranking Stage Dataset
Lower 0-20% percentile VEG Tachyzoite, ME49 Tachyzoite, ME49 merozoite, ME49 Bradyzoite. Gregory Hehl AB Sibley/Greg
Show/Hide expression data references
  • Sibley/Greg ToxoDB
  • Gregory ToxoDB
  • Fritz HM Transcriptomic analysis of toxoplasma development reveals many novel functions and structures specific to sporozoites and oocysts.
  • Hehl AB Asexual expansion of Toxoplasma gondii merozoites is distinct from tachyzoites and entails expression of non-overlapping gene families to attach, invade, and replicate within feline enterocytes.

Orthologs

Ortholog group members (OG5_139606)

Species Accession Gene Product
Babesia bovis BBOV_III006360   LCCL domain containing protein
Cryptosporidium hominis Chro.70203   A135 protein
Cryptosporidium parvum cgd7_1730   CpCCp1/Cpa135
Neospora caninum NCLIV_048780   LCCL domain containing protein, related
Neospora caninum NCLIV_024000   hypothetical protein
Plasmodium berghei PBANKA_1300700   LCCL domain-containing protein
Plasmodium falciparum PF3D7_1475500   LCCL domain-containing protein
Plasmodium knowlesi PKNH_1245800   LCCL domain-containing protein
Plasmodium vivax PVX_118650   LCCL domain-containing protein
Plasmodium yoelii PY05554   LCCL domain-containing protein CCP2
Toxoplasma gondii TGME49_264070   F5/8 type C domain-containing protein
Toxoplasma gondii TGME49_223700   F5/8 type C domain-containing protein
Toxoplasma gondii TGME49_264075   hypothetical protein
Theileria parva TP02_0326   hypothetical protein

Essentiality

TGME49_223700 has direct evidence of essentiality
Gene/Ortholog Organism Phenotype Source Study
PBANKA_1300700 Plasmodium berghei Dispensable plasmo
TGME49_264070 Toxoplasma gondii Essentiality uncertain sidik
TGME49_223700 this record Toxoplasma gondii Essentiality uncertain sidik
TGME49_264075 Toxoplasma gondii Essentiality uncertain sidik
TGME49_264070 Toxoplasma gondii Probably non-essential sidik
TGME49_223700 this record Toxoplasma gondii Probably non-essential sidik
TGME49_264075 Toxoplasma gondii Probably non-essential sidik
Show/Hide essentiality data references
  • yeastgenome Systematic deletion of yeast genes Saccharomyces Genome Database
  • shigen Profiling of E. coli Chromosome (PEC) National Institute of Genetics, Japan
  • nmpdr Genome-scale essentiality datasets from published studies (M. tuberculosis) National Microbial Pathogen Data Resource
  • goodall The Essential Genome of Escherichia coli K-12 (Transposon directed high-throughput mutagenesis) Goodall, Emily CA, et al. "The essential genome of Escherichia coli K-12." mBio 9.1 (2018): e02096-17.
  • gerdes Experimental determination and system-level analysis of essential genes in E. coli MG1655 Gerdes et al., J Bacteriol. 2003 185:5673-84
  • alsford High-throughput phenotyping using parallel sequencing of RNA interference targets in the African trypanosome Genome Res 2011, 21:915-924
  • keio Systematic single-gene knock-out mutants of E. coli K12 The Keio Collection
  • plasmo Functional Profiling of a Plasmodium Genome Reveals an Abundance of Essential Genes. Bushell, Ellen, et al. "Functional profiling of a Plasmodium genome reveals an abundance of essential genes." Cell 170.2 (2017): 260-272.
  • neb C. elegans RNAi phenotypes Data obtained from Wormbase WS150, curated by K. Chaudary and T. Carlow, New England Biolabs
  • blattner Systematic mutagenesis of the E. coli (MG1655) genome J Bacteriol 2004, 186:4921-4930
  • wormbase C. elegans RNAi experiments WormBase web site, http://www.wormbase.org, release WS170
  • sidik A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik, Saima M., et al. "A genome-wide CRISPR screen in toxoplasma identifies essential apicomplexan genes." Cell 166.6 (2016): 1423-1435.

Phenotypes and Validation (curated)

We have no manually annotated phenotypes for this target. What does this mean? / Care to help?

In TDR Targets, information about phenotypes that are caused by drugs, or by genetic manipulation of cells (e.g. gene knockouts or knockdowns) is manually curated from the literature. These descriptions help to describe the potential of the target for drug development. If no information is available for this gene or if the information is incomplete, this may mean that i) the papers containing this information either appeared after the curation effort for this organism was carried out or they were inadvertently missed by curators; or that ii) the curation effort for this organism has not yet started.

In any case, if you have information about papers containing relevant validation data for this target, please contact us.

Annotated validation

No validation data for this target

Associated compounds / Druggability

Known modulators for this target

No chemical compounds associated to this gene

Predicted associations

By orthology with druggable targets
Non orthologous druggable targets
By sequence similarity to non orthologous druggable targets
No additional associated druggable targets
Obtained from network model

Ranking Plot

Putative Drugs List

Compound Raw Global Species
0.007 0.4079 1
0.0254 0.3124 0.7933
0.0283 0.4079 0.5
0.0305 0.5261 0.5
0.0229 0.4079 0.5
0.0254 0.3124 0.7933
0.0305 0.5261 0.5
0.0257 0.601 0.5
0.0254 0.3124 0.7933
0.02 0.3008 0.4822
0.021 0.6221 1
0.0254 0.3124 0.7933
0.0058 0.5947 0.5
0.0111 0.3516 0.5
0.0254 0.3124 0.7933
0.0111 0.3516 0.5
0.0234 0.5952 0.5
0.0046 0.4809 0.5

Assayability

Assay information

No assay information for this target.

Reagent availability

No reagent availability information for this target.

Bibliographic References

No literature references available for this target.

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Gene identifier TGME49_223700 (Toxoplasma gondii), F5/8 type C domain-containing protein
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